1. Default LSR II Configuration BLUE 488nm PerCP, PerCP-Cy5.5, PE-Cy5
LSRII default 488
FITC
Alexa488
695/40
685 LP
530/30
505LP
488/10
550 LP
SSC
735 LP
576/26
780/60 PE
PE-Cy7

2. Default LSR II Configuration For A700 we need: 710/50, LP685 in B
LSRII default 633, 407
RED
633nm
APC
VIOLET
407nm
PacificBlue, Alexa405
660/20
440/40
735 LP
505 LP
780/60
525/50
APC-Cy7, A750
AmCyan, Alexa430, Cascade Yellow,
CFP

3. Default LSR II Configuration
LSRII default 355
Hoechst (blue)
DAPI UV
355nm
440/40
505 LP
530/30

4. GFP/YFP LSR II Configuration PerCP, PerCP-Cy5.5, PE-Cy5
LSRII YFP/GFP
GFP
695/40
685 LP
510/20
505LP
488/10
SSC
525 LP
735 LP
550/30
780/60
YFP
PE-Cy7

5. LSR II Configuration for Calcium Flux Assays Stem Cell Side Population
LSRII calcium, SP
Stem Cell Side Population
Dead Cell Exclusion
Hoechst (blue)
DAPI
Indo-1 violet UV
355nm UV
355nm
440/40
405/20
505 LP
450 LP
530/30
585/42
Indo-1 blue
Hoechst (red)
PI (UV)

6. Default FACSAria Configuration Use Aria 7-3-08 BLUE 488nm
PerCP, PerCP-Cy5.5, PE-Cy5
Aria default 488 PE
695/40
655 LP
576/26
556LP
530/30
502LP
FITC
Alexa488
488/10
SSC
595 LP
755 LP
610/20
780/60
PE-TexasRed
PE-Cy7

7. Default FACSAria Configuration PacificBlue, Alexa405
Hoechst Blue, DAPI
Alexa700
APC-Cy5.5
RED
633nm
VIOLET
407nm
720/20
440/40
680 LP
735 LP
502 LP
660/20
780/60
585/42
AmCyan, Alexa430,
Cascade Yellow, CFP,
Pacific Orange
APC
Alexa647
APC-Cy7
Aria default 633, 407

8. FACSAria GFP/YFP Configuration PerCP, PerCP-Cy5.5, PE-Cy5
695/40
685 LP
550/30
525 LP
510/20
505LP
GFP
600 LP
735 LP
610/20
780/60
PE-TexasRed
PE-Cy7

9. FACSAria Stem Cell Side Population Dead Cell Exclusion
Aria SP
VIOLET
Hoechst Blue
440/40
505 LP
585/42
Hoechst Red
PI (violet)

10. Dyes, Alternative Names, Filters
Part I Antibody Conjugates
FITC 530/30
PE 576/26
PE-TexasRed (ECD, Red610) 610/20
PerCP 695/40
PerCP-Cy /40
PE-Cy5 (CyChome, PC5, TriColor, Red670) 695/40
PE-Cy7 780/60
APC 660/20
APC-Cy /20
Alexa /20
APC-Cy7 (PharRed) 780/60
AmCyan 530/30
Alexa /30
PacificBlue 440/40
Alexa /40

11. Dyes, Alternative Names, Filters
Part II DNA Probes, Fluorescence Reporters, Functional Dyes
GFP with YFP: 510/20 (change mirror to 525LP) only GFP: 530/30
YFP with GFP: 550/30 (change mirror to 525 LP) only YFP: 576/26
DsRed2 Aria: 610/20 or 576/26 LSR II: 576/26 or 585/42 (change PE filter)
CFP 530/30 (violet laser)
Hoechst blue 440/40
Hoechst red 585/42
PI (blue laser) Aria: 610/20 (PE-TexasRed) LSR II 695/40 (PerCP) CellCycle
PI (Aria violet or LSR II UV) 585/42 Dead Cell Exclusion
Indo-1 Calcium dye blue LSR II only 405/20 (insert 450 LP mirror instead of 505 LP!)
Indo-1 Calcium dye violet LSR II only 530/30 (insert 450 LP mirror instead of 505 LP!)