1. Fluorescent principle

2. Fluorescent microscope

3. DAPI

4. FITC

5. Alexa 488

6. Tetramethylrhodamine/TRITC

7. Rhodamin

8. Rhodamin Red-X

9. Alexa 568

10. Alexa 647

11. Texas-Red and Texas-Red-X

12. Alexa 594

13. Cy-Series

14. GFP and GFPuv

15. E-GFP's and DsRed

16. Table of Fluorescent Proteins

17. What is green fluorescent protein (GFP) ? GFP is a 238-amino acid protein originally isolated from the bioluminescent jellyfish Aequoria victoria Can be expressed in any organism without additional cofactors

18. Structurally, GFP represents a new motif which has been named the “β-can” The “can” consists of 11 β-strands and a central α-helix Structure of GFP

19. GFP-Fluorophore Fluorescence is mediated by a fluorophore located at the center of the protein

20. The fluorophore is derived from the tripeptide -Ser 65 -Tyr 66 - Gly 67 - Formation of the fluorophore occurs in an autocatalytic manner Structure of the GFP-fluorophore

21. Light sources for Fluorescence

22. Filtersets and GFP's

23. Quantum efficiency of Chip

24. New Filterset

25. New Filterset-other flourochromes

26. Old Filtersets

27. Old filtersets- other flurochromes

28. Antibodies used for fluorescence

29. Laser and Fluorochromes