1. Products > CHO-K1 Transfection Reagent (Ovarian Adenocarcinoma)
Altogen labs
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO-K1 Transfection Reagent (Ovarian Adenocarcinoma)
Altogen Biosystems offers the CHO-K1 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits.
The CHO-K1 Transfection Reagent is an advanced formulation of a liposome based lipid reagent, and it has been developed to provide high transfection efficiency with the CHO-K1 cell line.
This cell line is a good transfection host and a tool for research related to ovarian adenocarcinoma. When cultured in vitro, CHO-K1 cells are a monolayer and adhere to the surface of the culture flask.
Other applications include drug discovery, gene expression studies, molecular and cell biology research applications.
Purchase CHO-K1 Transfection Kit at
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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2. High Density CHO-K1 cells
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO-K1 Transfection Reagent (Ovarian Adenocarcinoma)
CHO-K1 Cell Line Description and Applications
The CHO-K1 cell line originates from a subclone of the CHO cell line, a cell line established in 1957 and derived from the ovarian tissue of an adult Chinese hamster (Cricetulus griseus). The CHO-K1 cell line actually requires proline for cell growth, but nonetheless makes an excellent transfection host. The cell line is resistant to poliovirus 2, Modoc virus and Button Willow virus. The CHO-K1 cell line was mutagenized with ethyl methane sulfonate and this generated a cell line lacking DHFR.
High Density CHO-K1 cells
Atcc.org
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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3. CHO-K1 Transfection Protocol
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO-K1 Transfection Reagent (Ovarian Adenocarcinoma)
CHO-K1 Transfection Protocol
1. Plate 10, ,000 CHO-K1 cells per well in 0.5 ml of complete growth medium 12–24 hours prior to transfection
2. Wash with 1xPBS and add 0.5 ml of fresh growth medium
3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent, and
• 750 ng DNA (or mRNA), or
• 30 nM - 50 nM of siRNA (or microRNA)
*Referred to a final volume including growth medium
4. Incubate transfection complexes at RT for minutes
5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however it may increase cell toxicity
6. Add prepared transfection complexes to 0.5 ml of complete growth medium with CHO-K1 cells (from step 2)
7. Incubate cells at 37ºC in a humidified CO2 incubator
8. Assay for phenotype or target gene expression hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent hours after transfection
If the viability of CHO-K1 cells being transfected is affected at hours post-transfection, the level of cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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4. General Lipoplex-mediated Transfection Mechanism of Action
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO-K1 Transfection Reagent (Ovarian Adenocarcinoma)
General Lipoplex-mediated Transfection Mechanism of Action
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   

5. CHO-K1 Transfection Kit Product Details
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO-K1 Transfection Reagent (Ovarian Adenocarcinoma)
CHO-K1 Transfection Kit Product Details
A liposome-based lipid formulation
Optimized for intracellular delivery of plasmid DNA, siRNA, microRNA, and mRNA
High transfection efficiency of both siRNA and plasmid DNA without compromising cell viability
Achieve robust siRNA uptake for dependable gene silencing
Effective transfection under conditions of up to 40% serum
Transfection kit includes Transfection Enhancer reagent
Gentle enough to be used for single cell analysis
Developed and manufactured by Altogen Biosystems (
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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6. Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO-K1 Transfection Reagent (Ovarian Adenocarcinoma)
Data
Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into CHO-K1 cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).

7. Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO-K1 Transfection Reagent (Ovarian Adenocarcinoma)
Data
Figure 2. Protein expression of Lamin A/C in CHO-K1 cells. DNA plasmid expressing Lamin A/C or siRNA targeting Lamin A/C were transfected into CHO-K1 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

8. CHO-K1 Transfection Kit Benefits
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO-K1 Transfection Reagent (Ovarian Adenocarcinoma)
CHO-K1 Transfection Kit Benefits
Pre-optimized transfection protocol for CHO-K1 cell line
Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
Free of serum and protein of animal origin
Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
Easy to use CHO-K1 transfection protocol ensures great performance with expedited experimental timeline
Equally efficient for single or multiple transfections
Can be used for transient transfection and development of stable CHO-K1 cell lines
Bio-degradable after endocytosis
Used for preclinical research worldwide
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   