1. Products > CHO Transfection Reagent (Chinese Hamster Ovary Cells)
Altogen labs
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO Transfection Reagent (Chinese Hamster Ovary Cells)
Altogen Biosystems offers the CHO Cell Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits.
The CHO Cell Transfection Reagent is a lipid mediated formulation, and it has been developed to provide high transfection efficiency with CHO.
This cell line is a good host for studying a range of areas within the field of biomedical therapeutics and biology. When cultured in vitro, CHO cells are a monolayer and adhere to the surface of the culture flask.
Other applications include drug discovery, gene expression studies, molecular and cell biology research applications.
Purchase CHO Transfection Kit at
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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2. CHO cells www.microscopyu.com
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO Transfection Reagent (Chinese Hamster Ovary Cells)
CHO Cell Line Description and Applications
The CHO or Chinese Hamster Ovary cell line was established using ovaran cells from a Chinese Hamster (Cricetulus griseus) in Chinese Hamster Ovary cells display epithelial morphology. This cell line has a far reach as it is widely used in transfection and the production of therapeutic proteins and recombinant proteins. Beyond commercial applications, the CHO cell line has application for biological and biomedical research. For example, the cell line can be used in studies ranging from toxicity screening to gene expression and nutrition.
CHO cells
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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3. CHO Transfection Protocol
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO Transfection Reagent (Chinese Hamster Ovary Cells)
CHO Transfection Protocol
1. Plate 10, ,000 CHO cells per well in 0.5 ml of complete growth medium 12–24 hours prior to transfection
2. Wash with 1xPBS and add 0.5 ml of fresh growth medium
3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent, and
• 750 ng DNA (or mRNA), or
• 30 nM - 50 nM of siRNA (or microRNA)
*Referred to a final volume including growth medium
4. Incubate transfection complexes at RT for minutes
5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however it may increase cell toxicity
6. Add prepared transfection complexes to 0.5 ml of complete growth medium with CHO cells (from step 2)
7. Incubate cells at 37ºC in a humidified CO2 incubator
8. Assay for phenotype or target gene expression hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent hours after transfection
If the viability of CHO cells being transfected is affected at hours post-transfection, the level of cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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4. General Lipoplex-mediated Transfection Mechanism of Action
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO Transfection Reagent (Chinese Hamster Ovary Cells)
General Lipoplex-mediated Transfection Mechanism of Action
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   

5. CHO Transfection Kit Product Details
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO Transfection Reagent (Chinese Hamster Ovary Cells)
CHO Transfection Kit Product Details
Two component formulation enhances lipid mediated transfection efficiency
Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
Kit includes Transfection Enhancer reagent and recommended transfection protocol
High transfection efficacy in the presence of serum
Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
Reproducible transfection results
Works well for standard reverse transfection and high-throughput applications
Developed and manufactured by Altogen Biosystems (
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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6. Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO Transfection Reagent (Chinese Hamster Ovary Cells)
Data
Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the CHO cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPDH mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=3).

7. Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO Transfection Reagent (Chinese Hamster Ovary Cells)
Data
Figure 2. Protein expression of GAPDH in CHO cells. DNA plasmid expressing GAPDH or siRNA targeting GAPDH were transfected into CHO cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

8. CHO Transfection Kit Benefits
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO Transfection Reagent (Chinese Hamster Ovary Cells)
CHO Transfection Kit Benefits
Pre-optimized transfection protocol for CHO cell line
Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
Free of serum and protein of animal origin
Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
Easy to use CHO transfection protocol ensures great performance with expedited experimental timeline
Equally efficient for single or multiple transfections
Can be used for transient transfection and development of stable CHO cell lines
Bio-degradable after endocytosis
Used for preclinical research worldwide
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   