1. Copyright © 2006 American Medical Association. All rights reserved.
From: Bone Marrow–Derived Cells in Normal Human Corneal Stroma
Arch Ophthalmol. 2006;124(1): doi: /archopht
Figure Legend:
Immunohistochemical staining of CD45-positive cells in normal human corneal stroma. The transverse cross sections stained with anti-CD45 monoclonal antibody were coverslipped using antifading mounting medium containing propidium iodide (PI) (red) for nuclear staining. Representative staining shows the presence of CD45-positive cells (green) in the anterior stroma of the central (A) and paracentral (B) corneal stroma and all stromal layers of the peripheral cornea (C) (A-C, original magnification × 100). Confocal microscopic examination of 40-μm, sliced, flat-mount corneal stroma shows that these CD45-positive cells are mainly round or spindle form in the normal corneal stroma (D) (total thickness 40 μm; original magnification × 200). CD45-positive and PI-positive cells in the peripheral, paracentral, and central corneal stromal area were counted in cross sections from 5 donor corneas, and the percentage of CD45-positive cells was determined by dividing the number of CD45-positive cells by the number of PI-positive cells in each corneal stromal area (E).
Date of download: 10/25/2017
Copyright © 2006 American Medical Association. All rights reserved.

2. Copyright © 2006 American Medical Association. All rights reserved.
From: Bone Marrow–Derived Cells in Normal Human Corneal Stroma
Arch Ophthalmol. 2006;124(1): doi: /archopht
Figure Legend:
Representative immunostaining photographs of CD45-positive cells with various markers. In corneal cross sections, all CD45-positive cells (green) (A) are stained with CD11b (red) (B) in the anterior stroma of the central area. Double-stained cells are observed as yellow cells (C). White arrows indicate the corneal endothelial cell layer (A). CD45-positive cells (green) are totally CD11c-positive (red) in all layers of the peripheral stroma (yellow) (D). Similar CD45- and CD11c-positive cells were observed in the central and paracentral corneal stroma. A few CD45-, CD11b-, and CD11c-positive cells were also detected in the posterior layer of the center and paracentral stroma (data not shown). All CD11c-positive cells (red) are CD14 positive (green) in the corneal stroma. E, A representative merged photograph (yellow) (A-E, original magnification ×100).
Date of download: 10/25/2017
Copyright © 2006 American Medical Association. All rights reserved.

3. Copyright © 2006 American Medical Association. All rights reserved.
From: Bone Marrow–Derived Cells in Normal Human Corneal Stroma
Arch Ophthalmol. 2006;124(1): doi: /archopht
Figure Legend:
HLA-DR antigen expression in the corneal stroma. A, HLA-DR antigen–positive cells (green) are detected in the anterior stroma of the central and paracentral stroma and all layers of the peripheral stroma. This representative photograph shows that HLA-DR antigen–positive cells (green) are detected with anti-DR antigen monoclonal antibody (clone TAL.1B5) in the central corneal stroma. B, These HLA-DR antigen–positive cells are CD11b positive (red-yellow, double labeled). C, HLA-DRa antigen messenger RNA is detected in the CD45-positive, but not CD45-negative, stromal cells derived from the 3-mm diameter of the central stroma (35 cycles). Glyceraldehyde-phosphate dehydrogenase (GAPDH), served as endogenous reference, is detected in CD45-positive and -negative stromal cells (30 cycles). No polymerase chain reaction products are detected in the negative control sample (A and B, original magnification ×100). bp indicates base pair.
Date of download: 10/25/2017
Copyright © 2006 American Medical Association. All rights reserved.

4. Copyright © 2006 American Medical Association. All rights reserved.
From: Bone Marrow–Derived Cells in Normal Human Corneal Stroma
Arch Ophthalmol. 2006;124(1): doi: /archopht
Figure Legend:
Toll-like receptor (TLR) expression by flow cytometry and reverse- transcriptase polymerase chain reaction. Expression of TLR4 expression was examined in CD45-positive and -negative cells separated from all corneal stromas with magnetic beads. A, Using flow cytometry, TLR4 is detected in CD45-positive, but not CD45-negative, cells. Open and dotted histograms represent cells stained with respective isotype-matched mouse IgG2a and anti-TLR4 monoclonal antibody. B, Both TLR4 and glyceraldehyde-phosphate dehydrogenase (GAPDH) messenger RNAs are detected in the CD45-positive cells, whereas TLR4 messenger RNA is not detected in the CD45-negative cells (30 cycles). The TLR4 and GAPDH complementary DNAs (cDNAs), provided by the manufacturer, served as positive controls. bp indicates base pair.
Date of download: 10/25/2017
Copyright © 2006 American Medical Association. All rights reserved.