1. From: Methylene Blue Protects Primary Rat Retinal Ganglion Cells from Cellular Senescence
Invest. Ophthalmol. Vis. Sci ;53(8): doi: /iovs
Figure Legend:
MB significantly increases cytochrome c oxidase activity during hydrogen peroxide toxicity. MB significantly protects cytochrome c activity in RGCs exposed to hydrogen peroxide; 400,000 primary rat RGCs were cultured for 7 days before being subjected to treatment. Primary RGCs were treated for 120 minutes with a vehicle or 500 μM hydrogen peroxide with or without 1 μM MB. Cytochrome c oxidase activity was measured by a cytochrome c oxidase assay kit (CYTOCOX1; Sigma-Aldrich) following the manufacturer's protocol. This assay uses cytochrome c oxidase activity to convert ferrocytochrome c, which has an absorbance at 550 nm, to ferricytochrome c, which does not have an absorbance at 550 nm. A spectrophotometer was used to measure ferrocytochrome at 550 nm for the course of 120 seconds. The faster the rate of change in absorbance at 550 nm indicates a larger amount of cytochrome c activity. Results indicate that primary RGCs had the highest functioning cytochrome c oxidase, whereas hydrogen peroxide decreased it. The addition of MB increased the amount of functioning cytochrome c oxidase. The control RGC cytochrome c oxidase activity was set to the arbitrary unit of 1, whereas every experimental group was expressed as a percentage of control. Hydrogen peroxide significantly decreased cytochrome c oxidase activity by 69% ± 7% of the control (P = 0.029). The addition of MB significantly preserved cytochrome c oxidase activity 91% ± 3 % (P = 0.037) against the hydrogen peroxide insult (n = 4).
Date of download: 10/21/2017
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