1. Influence of conjugated linoleic acid (CLA) supplementation on Haptoglobin and Leptin during the lactation period in dairy cattle
Amani Al-Dawood
Supervised by Prof. Dr. Dr. Sauerwein

2. Objectives
To assess the potential effects of long-term CLA supplementation during the lactation period on:
1 - Haptoglobin as inflammation marker.
2 - Adipokines (leptin).

3. Experiment 1….Results…Hp…All animals
Group p=0.646
Sample p=0.000
n=49 animals
Control
50g CLA
Calving
Stop CLA feeding
≥ 0.15 mg/ml
< 0.15 mg/ml
Control
108
180
50 g CLA 93
194
100 g CLA
105
192
100g CLA
Mean Hp concentration (mg/mL)
P=0.039
Days relative to parturition
Figure 1: Mean (±SEM) Haptoglobin concentration (mg/mL) by feeding dairy cattle on two different concentrations of CLA and control treatment in relative to parturition.

4. Control 50 g CLA 100 g CLA Muscle absces Mastitis Endometritis
Mean Hp concentration (mg/mL)
Endometritis
Metritis
50 g CLA
Endometritis
Mastitis
Mean Hp concentration (mg/mL)
Muscle absces
100 g CLA
Absces
Mean Hp concentration (mg/mL)
Mastitis
Mastitis
Days relative to parturition

5. Mean Hp concentration (mg/mL)
Experiment 2….Results…Hp
n=25 animals ns
Mean Hp concentration (mg/mL)
Days relative to parturition
Figure 2: Mean (±SEM) Hp concentration (mg/mL) in control group and CLA treatment.
ns= not significant

6. Day 1 Day 42 con Day 42 CLA Day 105 con Day 105 CLA
Mean Hp concentration (mg/mL)
Day 42 con
Day 42 CLA
Mean Hp concentration (mg/mL)
Mean Hp concentration (mg/mL)
Day 105 con
Day 105 CLA
Mean Hp concentration (mg/mL)
Mean Hp concentration (mg/mL)
Days relative to parturition
Days relative to parturition

7. leptin concentration (ng/mL) Days relative to parturition
Experiment 1…Results…Leptin…All animals
n=49 animals
Control
Calving
Stop CLA feeding
Group p=0.012
Con with 50 p=0.021
50 with p=0.038
Sample p=0.000
50g CLA
100g CLA
leptin concentration (ng/mL)
Days relative to parturition
Figure 3: Mean (±SEM) leptin concentration (ng/mL) by feeding dairy cattle on two different
concentrations of CLA and control treatment in relative to parturition.

8. Thank you for your attention

9. J. Anim. Sci :1923–1932