1. The Role of a Glycoprotein K (gK) CD8+ T-Cell Epitope of Herpes Simplex Virus on Virus Replication and Pathogenicity
Invest. Ophthalmol. Vis. Sci ;50(6): doi: /iovs
Figure Legend:
Effect of gK peptides on virus replication in eyes of mice and rabbits. (A) Virus titers in tears of BALB/c mouse treated with gK peptide 2. One hour before ocular infection with 2 × 105 PFU/eye of HSV-1 strain McKrae, BALB/c mice received 100 μg/eye of gK peptide 2 in eye drops. Control mice received 100 μg/eye of gK peptides 1, gK peptide 3, gD peptide, or VSV-G peptide. Tear swabs were collected from infected eyes on day 1 after ocular infection and virus titers were determined by standard plaque assays. Each point represents the mean titer from 30 eyes from two separate experiments. (B) Virus titers in tears of BALB/c and C57BL/6 mice treated with the gK 8mer. All procedures were as in (A) except that the experimental group was gK 8mer and controls were BSA and the S-8mer. For the BALB/c mice, each point represents the mean of 110 eyes from seven separate experiments. For the C57BL/6 mice, each point represents the mean of 70 eyes from three separate experiments. (C) Virus titers in tears of immunized mice treated with gK 8mer. BALB/c mice were immunized three times with baculovirus expressed gK or once with live avirulent HSV-1 strain KOS. Three weeks after the last immunization, the mice were treated with gK 8mer, BSA, or S-8mer and ocularly infected. Each point represents the mean titer of 30 eyes. (D) Virus titers in tears of rabbits treated with gK 8mer. As in (B) and (C), New Zealand White rabbits were treated with the 8mer (or BSA control) and ocularly infected 1 hour later. Each point represents the mean titer from 30 eyes.
Date of download: 10/11/2017
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