1. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) Poster session 2

2. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) Monitoring of Wet Nitrogen deposition 1. Avila: through fall nitrogen monitoring in Holmoak forest 2. Izquieta-Rojano: through fall nitrogen monitoring in Holmoak forest Effecs of Wet N deposition On plants, seeds, mosses, insects 3. Cabal: N added to rosemary shrubs 4. Lecha: effects of N on seed-bank germination 5. Izquieta-Rojano: ecophysiological response of mosses to added N 6. De la Pedrosa: effects of N on the collembola community of the soil On soil 7. Rodriguez: N addition to soil reduces the soil carbon 8. Duran: N addition to soil and influences on the drying-rewetting cycle 9. Lo Cascio: effect of N deposition on soil respiration 10. Morillas: effect of N deposition on biocrust respiration Interactions with other stressors 11. Fusaro: interaction of N and drought in holmoak & fraxinus 12. Munzi: interactive effects of N deposition & drought stress on lichens

3. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) Effects of metals & POPS 13. Cogolludo: Effects of Pb nitrate on mosses 14. Paoli: metals effects on lichens 15. Piscopo: Effects of Cd on mussels fertility 16. Vannini: Effecs of glyfosate on lichens

4. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) Throughfall N deposition and canopy exchange in holm-oak forests: its seasonal dynamics A. Avila (1), L. Aguillaume (1), S.Izquieta (2), H. García-Gómez (3), D.Elustondo (2), J.M.Santamaría (2), R.Alonso (3) (1) CREAF, (2) Navarra University, (3) CIEMAT Space for a graphical abstract: ONLY ONE PICTURE and/or ONE TABLE. Text is not allowed. Please REMOVE THIS BLUE BOX and THIS TEXTBOX A Canopy Budget Model indicated higher N canopy retention in the wet seasons as expected, but contrary to expectations, N Dry Deposition was about equal (or lower) in dry compared to wet seasons WD DD TD WET seasonLCCBCATCLCCBCATCLCCBCATC NH 4 + -N2,31,44,80,32,02,73,90,34,34,18,70,7 NO 3 - -N1,71,41,60,43,13,52,11,14,84,93,71,4 sum N4,02,86,40,75,16,26,01,49,19,012,42,1 DRY season NH 4 + -N1,91,32,30,22,34,22,20,34,25,54,50,5 NO 3 - -N1,20,81,10,32,93,41,80,64,14,22,90,9 sum N3,12,13,40,55,37,64,01,08,39,77,41,5 Annual dep NH 4 + -N4,22,77,10,54,36,96,10,78,59,613,21,2 NO 3 - -N2,92,12,70,76,06,93,91,78,99,16,62,3 sum N7,14,99,81,210,413,810,02,417,418,719,83,5 Wet, Dry and Total deposition by seasons (Kg ha -1 y -1 )

5. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) Agricultural activities and pollutants generated in metropolitan areas were identified as potential anthropogenic sources of DON. Organic fraction may be a supplementary nutrient for Mediterranean forests Throughfall and bulk deposition of dissolved organic nitrogen to holm oak forests in the Iberian Peninsula: flux estimation and identification of potential sources S. Izquieta-Rojano 1, H. García-Gómez 2, L. Aguillaume 3, J.N. Cape 4, D. Elustondo 1 et al. 1 University of Navarra (SPAIN), 2 CIEMAT (SPAIN), 3 CREAF (SPAIN), 4 CEH (UK) NTF = TF - BD Sowing time Inorganic fertilizer Urea fertilizer Harvest season

6. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) Effecs of Wet N deposition On plants, seeds, mosses, insects

7. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) High levels of nitrogen deposition (over ±10-15 kg N.ha -1.yr -1 ) revert the successional trend of colonization by shrubs, not by inhibiting the growth of shoots but due to defoliation and dead of adult parts of the plants. The effect of nitrogen deposition on rosemary shrubs dynamics C. CABAL 1, R. OCHOA-HUESO 2, E. PEREZ-CORONA 3, E. MANRIQUE 1 1 MNCN-CSIC (Madrid, Spain) | 2 HIE-WSU (Sydney, Australia) | 3 F. CC. Biológicas UCM (Madrid, Spain)

8. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) Atmospheric N deposition, particularly reduced forms, will affect plant germination and composition of seed banks in Mediterranean ecosystems with consequences in terms of altered plant community composition. Seed bank and seed germination are affected by Nitrogen deposition and different N forms L. Lecha 1, E. Perez-Corona 1, A. Lafuente 2, R. Ochoa-Hueso 3, E. Manrique 4 1 Universidad Complutense, 2 Universidad Rey Juan Carlos, 3 University of Western Australia, 4 MNCN-CSIC Number of germinated seeds Nitrogen treatment Kg N m -2 y -1

9. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) N accumulation and oxidative stress were the most important drivers of the physiological functioning. SOD, tissue N and  13 C showed the greatest potential for identifying NH 3 -related ecological end points Integrated eco-physiological response of the moss Hypnum cupressiforme Hedw. to increased ammonia concentrations S. Izquieta-Rojano 1, M. López-Aizpún 1, E. Lasheras 1, R. Ochoa-Hueso 2, D. Elustondo 1 et al. 1 University of Navarra, 2 Hawkesbury Institute for the Environment Pearson's correlation coefficient [NH 3 ]SeptemberDecemberMarchJuneAnnual Distance -.0893**-0.880**-0.887**-0.899**-0.897** N 0.948**0.971**0.981**0.841*0.967** C -0.847*-0.4740.050-0.543-0.794* P 0.0600.770*0.4740.2890.555 Mg -0.805*-0.859*-0.863*-0.432-0.771* C:N -0.894**-0.930**-0.993**-0.789*-0.936** N:P 0.770*0.5210.5010.737 a N:K 0.920**0.924**0.875*0.897**0.937** PME 0.817*0.2500.748 a 0.796*0.797* NR -0.908** SOD 0.956**0.930**0.894**0.917**0.950** MDA 0.762*0.857*0.960**-0.2580.911** Proteins 0.890**0.4570.866*0.6590.881** Chla+b 0.6790.7470.763*0.857*0.834* Carotenoids 0.760*0.846*0.880**0.890*0.887**  15 N -0.912**-0.577-0.875**-0.816*-0.854*  13 C 0.783*0.951**0.939**0.767*0.967**

10. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) N deposition effects on soil collembolan communities operate at the functional group level, whereas the individual species seem to respond to N in a more stochastic way. Effects of nitrogen deposition on soil collembolans from a semiarid Mediterranean shrubland Authors: A.M. DE LA PEDROSA (1), M.J LUCIÁÑEZ (1), R. OCHOA-HUESO (2) (1) Autónoma de Madrid University, Spain. (2) Hawkesbury Institute for the Environment, Australia. E- mail address: mjose.luciannez@uam.esmjose.luciannez@uam.es

11. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) Effecs of Wet N deposition On soil

12. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) There is a need to consider varying responses among different tree species when predicting future consequences of N inputs on soil C storage Long-Term N additions reduce the lability of soil C in temperate forest soils depending on tree species composition A. Rodriguez, G.M. Lovett, K.C. Weathers, M.A. Arthur, P.H. Templer, C.L. Goodale, L.M. Christenson Center for functional Ecology, University of Coimbra, Portugal

13. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) Projected changes in drying-rewetting cycles will decrease soil NO 3 -N and mineralization rates, as well as alter soil-atmosphere greenhouse gases exchange, but N supply could modulate this effect. Nitrogen supply modulates the effect of climate change on soil C and N cycling and greenhouse gas exchange Durán J, Morillas L, Rodríguez A, Roales J, Gallardo A, Lovett GM, Groffman PM Center for Functional Ecology, University of Coimbra (Portugal) Soil pool Nutrient transformation GHGs exchange Graphical hypothesis

14. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) Nitrogen deposition and the spatial/temporal heterogeneity typical of Mediterranean ecosystems affected soil respiration rates, triggering contrasting responses between the two study sites and microsites. Contrasting effects of nitrogen deposition on soil respiration in two Mediterranean ecosystems M. Lo Cascio 1,2, L. Morillas 1, R. Ochoa-Hueso 3, E. Manrique 4, S. Munzi 5, J. Roales 1, D. Spano 1,2, S. Mereu 1, 2 1 University of Sassari. 2 CMCC, Italy. 3 University of Western Sydney. 4 CSIC, Spain. 5 University of Lisbon

15. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) CO 2 flux emitted by Soil, BC and Soil+BC are differently driven by soil temperature and moisture. Biocrusts can contribute to soil respiration responding to small water pulses even when deeper soil layers are inactive. Contribution of the biological crust (BC) to the soil CO 2 efflux in a Mediterranean ecosystem L. Morillas 1, V. Bellucco 1,2, M. Lo Cascio 1,2, S. Marras 1,2, D. Spano 1,2, S. Mereu 1,2 1 University of Sassari, Italy. 2 CMCC Foundation, Sassari, Italy

16. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) Effecs of Wet N deposition Interaction with other stressors

17. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) Effects of nitrogen deposition, drought and their interaction, on functional and structural traits of Fraxinus ornus L. and Quercus ilex L. L. FUSARO 1 *, E. SALVATORI 1, F. MANES 1 1 Department of Environmental Biology, Sapienza University of Rome, Rome, Italy Nitrogen + 30kg ha yr Water stress 20 days of irrigation suspension Q. ilex F. ornus Functional Traits Structural Traits + N H2OH2O N deposition has different effects on functional and structural traits of the two studied species. N can have role in water stress response increasing gas exchange or changing biomass allocation patterns ( Fusaro et al., 2016. Plant Biosystems. In Press. DOI: 10.1080/11263504.2016.1193070 ).     N*H 2 O N H2OH2O            PNPN gsgs vcvc  Po RGRDWSLAL A /S W [N] leaf

18. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) Photosynthetic parameters in sensitive lichen species respond to the interactive effects of nitrogen and water stress. Do lichens respond to synergistic effects of nitrogen and water stress? A case study from South Portugal S. Munzi1, C. Branquinho1, P. Pinho2, S. Loppi3, L. Paoli3 1cE3c, Universidade de Lisboa; 2CERENA-IST Universidade de Lisboa; 3Università di Siena * *

19. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) Effect of metals and POPs

20. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) Externally-supplied lead is not in correspondence with lead detected in living tissues in all three moss species. The use of these mosses in heavy metal biomonitoring must be cautious. SIMULATED ATMOSPHERIC DEPOSITION OF LEAD ON THREE COMMON MOSS SPECIES (FAM. POTTIACEAE) Jennifer Cogolludo, Nagore García Medina and Belén Estébanez Fac. Ciencias, Dpto. Biología, Universidad Autónoma de Madrid

21. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) We tested the competitive behaviour of divalent heavy metals (Cd, Cu, Pb and Zn) for binding sites in Xanthoria parietina. When metals were provided in combination, their bioaccumulation decreased. The competitive behaviour of trace elements during bioaccumulation in lichen thalli may lead to an underestimation of real deposition Luca Paoli, Andrea Vannini, Fabrizio Monaci, Stefano Loppi Department of Life Sciences, University of Siena, Siena, Italy

22. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) The alteration of sperm Protamine Like proteins state and their DNA binding ability for cadmium bioaccumulation in M. galloprovincialis gonad could be useful for new chromatin-based genotoxicity tests in biomonitoring programs INDUSTRIAL POLLUTION AND REPRODUCTION: INFLUENCE OF CADMIUM EXPOSURE ON IN VIVO AND IN VITRO MEDITERRANEAN MUSSEL GONAD AND SPERM Marina Piscopo et al. Dipartimento di Biologia, Università di Napoli Federico II Italy

23. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) Physiological and ultrastructural effects of glyphosate in the lichen Xanthoria parietina (L.) Th.Fr. A. Vannini 1, M. Guarnieri 1, L. Paoli 1, S. Sorbo 2, A. Basile 2, S. Loppi 1 1 University of Siena, Italy; 2 University of Naples, Italy Treatments of lichen thalli with glyphosate cause toxic effects at physiological and ultrastructural level Chl b Chl a β-caroten Control Exposed Ergosterol Control Exposed

24. “(E)merging directions on air pollution and climate change research in the Mediterranean ecosystems” POSTER Session 2016, 28-30 June – Brescia (I) Have a nice poster view!