1. Date of download: 7/7/2016 Copyright © ASME. All rights reserved. From: Incorporation, Release, and Effectiveness of Dexamethasone in Poly(Lactic-Co-Glycolic Acid) Nanoparticles for Inner Ear Drug Delivery J. Nanotechnol. Eng. Med. 2011;2(1):011013-011013-7. doi:10.1115/1.4002928 Sustained release of DEX-P and DEX-B from particles. These are from the representative runs. Although the total amount of DEX release varied, the very high burst release and the short time required for all samples to reach a plateau were consistent for these encapsulated drugs. Figure Legend:

2. Date of download: 7/7/2016 Copyright © ASME. All rights reserved. From: Incorporation, Release, and Effectiveness of Dexamethasone in Poly(Lactic-Co-Glycolic Acid) Nanoparticles for Inner Ear Drug Delivery J. Nanotechnol. Eng. Med. 2011;2(1):011013-011013-7. doi:10.1115/1.4002928 Sustained release of DEX-Ac from particles. The error bars were calculated from five duplicate runs. For the nanoparticles containing SPIONs, two duplicate runs were done, which is why there are two data points for each time increment. Release profiles for DEX-Ac and DEX-Ac/SPION nanoparticles are almost identical and showed that the incorporation of magnetite did not significantly affect the release kinetics of the drug. Both types of nanoparticles achieved sustained release over 2 weeks. The total amount of drugs encapsulated in this particle was 0.63 mg/mg particle for the non-SPION containing particle and 0.61 mg/mg particle for the SPION containing particle. Figure Legend:

3. Date of download: 7/7/2016 Copyright © ASME. All rights reserved. From: Incorporation, Release, and Effectiveness of Dexamethasone in Poly(Lactic-Co-Glycolic Acid) Nanoparticles for Inner Ear Drug Delivery J. Nanotechnol. Eng. Med. 2011;2(1):011013-011013-7. doi:10.1115/1.4002928 Protective effect of DEX-Ac nanoparticles on 4-HNE ototoxicity studied on cochlear culture from P3 CD-1 mouse pups. Apical turns are the top two figures. Below this, the middle turns are shown in the left column, while basal turns are presented in the right column. The OHC region is indicated by a bracket (]) and the IHC region is indicated by the arrow symbol (→). Figure Legend:

4. Date of download: 7/7/2016 Copyright © ASME. All rights reserved. From: Incorporation, Release, and Effectiveness of Dexamethasone in Poly(Lactic-Co-Glycolic Acid) Nanoparticles for Inner Ear Drug Delivery J. Nanotechnol. Eng. Med. 2011;2(1):011013-011013-7. doi:10.1115/1.4002928 Quantitative analysis of DEX-Ac particles on 4-HNE ototoxicity studied in the organ of Corti cultures from cochleae of P3 CD-1 mouse pups. Intact hair cell numbers were counted along 100 linear μm of tissue along the basilar membrane. As controls, the organ of Corti cultures without 4-HNE or nanoparticle treatment without DEX-Ac were included. The statistically significant differences in intact hair cell number per 100 μm between DEX-Ac particles+4-HNE group and the 4-HNE group were analyzed by ANOVA with the Tukey post-hoc test. The asterisks ( ∗, ∗∗, and ∗∗∗ ) represent p<0.05, 0.01, and 0.001, respectively. Figure Legend: