Presentation is loading. Please wait.

Presentation is loading. Please wait.

Kudo-Saito, et al Supplementary Figure 1 Leukocytes Lymphocytes Debris Dead cells Human PBMCs.

Published byOpal Ross Modified over 7 years ago

Similar presentations

Presentation on theme: "Kudo-Saito, et al Supplementary Figure 1 Leukocytes Lymphocytes Debris Dead cells Human PBMCs."— Presentation transcript:

1 Kudo-Saito, et al Supplementary Figure 1 Leukocytes Lymphocytes Debris Dead cells Human PBMCs

2 Panc1-mock D6-snail+ D10-snail+ F3-snail+ F5-snail+ Snail E-cadherin Fibronectin Morphology Proliferation InvasionAdhesion Mock D6 D10 F3 F5 NC Snail Slug Twist E-cadherin Fibronectin MMP2 Collagen IV GAPDH Transfectants Absorbance (570nm) No. of cells (a) (b) (c) 2.0 1.5 1.0 0.5 0.0 Normalization Snail 0.8 0.6 0.4 0.2 0.0 0.8 0.6 0.4 0.2 0.0 0.6 0.4 0.2 0.0 E-cadherin Fibronectin Mock D6 D10 F3 F5 400 300 200 100 0 Mock D6 D10 F3 F5 Mock D6 D10 F3 F5 Mock D6 D10 F3 F5 Kudo-Saito, et al Supplementary Figure 2 Absorbance (450-655nm) 1.5 1.0 0.5 0.0

3 Kudo-Saito, et al Supplementary Figure 3 Snail transfectants 0.8 0.6 0.4 0.2 0 CCL2 (a) 19.40 CCL2SnailF10-mockControl 17.7812.2562.47 Foxp3 in CD4 + cells Snail CCL2 LCN2 GAPDH H6 + siRNA ng/ml Mock E9 H6 Mock E9 H6 0.6 0.4 0.2 0.0 LCN2 Mock E9 H6 1.0 0.8 0.6 0.4 0.2 0.0 0.5 0.4 0.3 0.2 0.1 0.0 ng/ml Control Snail CCL2 Mock Control Snail CCL2 Mock H6 + siRNA (c) CCL2LCN2 H6 + siRNA Snail CCL2 LCN2 GAPDH H6 + siRNA Control Snail CCL2 #1 CCL2 #2 Mock (b)

4 ControlsiRNA-snailsiRNA-CCL2 2.53 5.13 3.61 4.48 9.1318.36 0.615.42 CD4 I-A(b) 1.15 1.67 2.67 1.49 B16-F10 tumors 64% (466) 62% (630) 60% (816) Foxp3 in CD4 + cells 79% (2058) 78% (1984) 81% (535) Gp70 tetramer in CD8 + cells CD11c (b) CD8 (a) 600 400 200 0 Tumor volume (mm 3 ) Control Snail CCL2 B16-F10 + siRNA (c) 800 600 400 200 0 IFN-γ (pg/ml) Control Snail CCL2 B16-F10 + siRNA Kudo-Saito, et al Supplementary Figure 4

5 Kudo-Saito, et al Supplementary Figure 5 ControlsiRNA-snailsiRNA-CCL2 0.07 0.13 1.17 2.38 1.85 2.09 1.055.994.12 2.1816.6210.26 CD4 I-A(b) 1.15 1.67 2.67 1.49 F10-mock H6-snail + tumors CD8 81.67 (1430) 82.17 (1180) 74.91 (1150) 60.43 (816) Foxp3 in CD4 + cells 13.34 (289) 64.92 (1521) 71.38 (2231) 81.29 (535) Gp70 tetramer in CD8 + cells CD11c

6 Kudo-Saito, et al Supplementary Figure 6 Snail + cancer cell undergoing EMT CCL2 release Recruitment of CD4 low FOXP3 + CCR2 + Treg Induction of CD4 + FOXP3 + Treg Induction of CD11c + HLA-DR low DCreg Inhibition of CTL induction LCN2 release (autocrine) Enhancement of cancer invasion

Download ppt "Kudo-Saito, et al Supplementary Figure 1 Leukocytes Lymphocytes Debris Dead cells Human PBMCs."

Similar presentations

Fig. S1 HUVECs HDFNs HUVECs HDFNs Transwell CM Relative total length ** * control 200ng/ml400ng/ml TIMP-1 100ng/ml * ** GFP WT Mutant TIMP-1 TIMP-1 **

Fig. S1 HUVECs HDFNs HUVECs HDFNs Transwell CM Relative total length ** * control 200ng/ml400ng/ml TIMP-1 100ng/ml * ** GFP WT Mutant TIMP-1 TIMP-1 **
Clancy-Thompson et al., Supplemental Figure 1 A B C Supplemental Figure 1: Mice were given intravenous B16 injection on day 0. A) After bearing tumors.

Clancy-Thompson et al., Supplemental Figure 1 A B C Supplemental Figure 1: Mice were given intravenous B16 injection on day 0. A) After bearing tumors.
Suppl. Figure 1 A B Suppl. Figure 1. Relative (A) miR-21 and (B) miR-224 expression levels in HCC tumors in different stages (I,II,III,IV) assessed by.

Suppl. Figure 1 A B Suppl. Figure 1. Relative (A) miR-21 and (B) miR-224 expression levels in HCC tumors in different stages (I,II,III,IV) assessed by.
Supplementary Figure 1 Supplementary Figure 1. Representative micrographs of migration and invasion assays. miR-452 or si-WWP1 transfectants inhibited.

Supplementary Figure 1 Supplementary Figure 1. Representative micrographs of migration and invasion assays. miR-452 or si-WWP1 transfectants inhibited.
Supplementary information Figure S1 Parental Mock ANXA1-V5 GAPDH MCF7-EV MCF7-V5 4 5 ANXA1 ANXA1-V5 Tubulin MCF7-parental MCF7-EV MCF7-V5 1 2 3 A B C ANXA1.

Supplementary information Figure S1 Parental Mock ANXA1-V5 GAPDH MCF7-EV MCF7-V5 4 5 ANXA1 ANXA1-V5 Tubulin MCF7-parental MCF7-EV MCF7-V A B C ANXA1.
Figure 2. Effects of RUNX1 and PU. 1 siRNAs on CCR3 reporter activity

Figure 2. Effects of RUNX1 and PU. 1 siRNAs on CCR3 reporter activity
Figure S1. qPCR analysis of the EMT markers ZEB2 and SNAI1 in Syndecan-1 and control siRNA transfected MDA-MB-231 and MCF-7 cells reveals no significant.

Figure S1. qPCR analysis of the EMT markers ZEB2 and SNAI1 in Syndecan-1 and control siRNA transfected MDA-MB-231 and MCF-7 cells reveals no significant.
Foxp3 induction dependent on cell cycle progression in vitro.

Foxp3 induction dependent on cell cycle progression in vitro.
Supplementary FigureS4 (Feduska et al.)

Supplementary FigureS4 (Feduska et al.)
a b SUM149PT Motility Invasion C V C V

a b SUM149PT Motility Invasion C V C V
Immunologic pathomechanism of Hodgkin's lymphoma

Immunologic pathomechanism of Hodgkin's lymphoma
FOXP3 regulates cAMP production by regulating miR‐142‐3p and AC9.

FOXP3 regulates cAMP production by regulating miR‐142‐3p and AC9.
SDHB deficiency promotes TGFβ-mediated invasion and metastasis of colorectal cancer through transcriptional repression complex SNAIL1-SMAD3/4  Haiyu Wang,

SDHB deficiency promotes TGFβ-mediated invasion and metastasis of colorectal cancer through transcriptional repression complex SNAIL1-SMAD3/4  Haiyu Wang,
Anti–4-1BB/PD-1 combination enriched CD8+ T cells in TILs

Anti–4-1BB/PD-1 combination enriched CD8+ T cells in TILs
Immunologic pathomechanism of Hodgkin's lymphoma

Immunologic pathomechanism of Hodgkin's lymphoma
Treg's Alter Ego: An Accessory in Tumor Killing

Treg's Alter Ego: An Accessory in Tumor Killing
Marissa V. Powers, Paul A. Clarke, Paul Workman  Cancer Cell 

Marissa V. Powers, Paul A. Clarke, Paul Workman  Cancer Cell 
Cell signaling and cancer

Cell signaling and cancer
Tailor-Made Renal Cell Carcinoma Vaccines

Tailor-Made Renal Cell Carcinoma Vaccines
Induction of antigen-specific regulatory T lymphocytes by human dendritic cells expressing the glucocorticoid-induced leucine zipper by Haifa Hamdi, Véronique.

Induction of antigen-specific regulatory T lymphocytes by human dendritic cells expressing the glucocorticoid-induced leucine zipper by Haifa Hamdi, Véronique.

Similar presentations

Ads by Google