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An antiapoptotic role for telomerase RNA in human immune cells independent of telomere integrity or telomerase enzymatic activity by Francesca S. Gazzaniga,

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Presentation on theme: "An antiapoptotic role for telomerase RNA in human immune cells independent of telomere integrity or telomerase enzymatic activity by Francesca S. Gazzaniga,"— Presentation transcript:

1 An antiapoptotic role for telomerase RNA in human immune cells independent of telomere integrity or telomerase enzymatic activity by Francesca S. Gazzaniga, and Elizabeth H. Blackburn Blood Volume 124(25):3675-3684 December 11, 2014 ©2014 by American Society of Hematology

2 hTR overexpression protects from dexamethasone-induced apoptosis independent of telomerase activity. Francesca S. Gazzaniga, and Elizabeth H. Blackburn Blood 2014;124:3675-3684 ©2014 by American Society of Hematology

3 hTR knockdown induces Bim-mediated apoptosis. Francesca S. Gazzaniga, and Elizabeth H. Blackburn Blood 2014;124:3675-3684 ©2014 by American Society of Hematology

4 Telomerase knockdown does not induce significant telomere shortening or telomere DNA damage-induced foci (TIFs) in the time frame of this experiment. Francesca S. Gazzaniga, and Elizabeth H. Blackburn Blood 2014;124:3675-3684 ©2014 by American Society of Hematology

5 hTERT overexpression increases telomerase activity and induces apoptosis. Francesca S. Gazzaniga, and Elizabeth H. Blackburn Blood 2014;124:3675-3684 ©2014 by American Society of Hematology

6 Overexpression of catalytically inactive hTR mutants protects from hTERT-induced apoptosis. Francesca S. Gazzaniga, and Elizabeth H. Blackburn Blood 2014;124:3675-3684 ©2014 by American Society of Hematology

7 Two functions for hTR. hTR and hTERT complex to form catalytically active telomerase to maintain telomeres. Francesca S. Gazzaniga, and Elizabeth H. Blackburn Blood 2014;124:3675-3684 ©2014 by American Society of Hematology


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