2. ERYTHROBLASTOSIS FETALIS SUPRIYA SARASWATI A potentially life threatening blood disorder in a foetus or a newborn, arising due to incompatibility with mother’s blood.‏ DIAGNOSIS OF THE DISEASE

3. Master layout or diagram Make a schematic diagram of the concept Explain the animator about the beginning and ending of the process. Draw image big enough for explaining. In above image, identify and label different components of the process/phenomenon. (These are like characters in a film)‏ Illustrate the basic flow of action by using arrows. Use BOLD lines in the diagram, (minimum 2pts.)‏ In the slide after that, provide the definitions of ALL the labels used in the diagram 5 3 2 4 1 INSTRUCTIONS SLIDE

4. MASTER LAYOUT Part Three: DIAGNOSIS OF EF 1. MATERNAL SERUM SCREEN 2. PERCUTANEOUS UMBILICAL CORD BLOOD SAMPLING (PUBS)‏

5. Definitions of the components : 5 3 2 4 1 1. Maternal serum screen: Testing mother’s serum at various intervals during pregnancy for antibodies to Rh antigen. 2. Fetal blood analysis: Testing fetus’s blood for presence of maternal IgG on the surface of fetal red blood cells.

6. Explain the process 1 5 3 2 4 In this step, use an example to explain the concept. It can be an analogy, a scenario, or an action which explains this concept/process/topic Try to use examples from day-to-day life to make it more clear You have to describe what steps the animator should take to make your concept come alive as a series of moving images. Keep the examples simple to understand, and also to illustrate/animate.

7. Analogy / Scenario / Action 1 5 3 2 4 The immune system of the body is well developed to fight infections and keep the body healthy. Any foreign substance (antigen) entering the body makes the immune system produce molecules called antibodies that destroy the antigen. Blood transfusions are procedures of transferring blood or blood based products from one circulatory system into another. For this the blood of the donor should be compatible with that of the recipient’s blood, which means the blood types should match. Or else any component not matching the recipient’s blood type will lead to production of antibodies in his body against the donor’s blood cells. These antibodies will attack the newly received blood and kill the blood cells. Erythroblastosis fetalis is a diseased condition arising from incompatibility of fetal blood with that of the maternal blood. It usually happens in Rh- women carrying Rh+ fetus. Maternal antibodies attack the fetal blood cells and destroy them.

8. Stepwise description of process The goal of the IDD is to provide instructions to an animator who is not a expert. You have to describe what steps the animator should take to make your concept come alive as a moving visualization. Use one slide per step. This will ensure clarity of the explanation. Add a image of the step in the box, and the details in the table below the box. You can use any images for reference, but mention about it's copyright status The animator will have to re-draw / re-create the drawings Add more slides as per the requirement of the animation 1 5 3 2 4

9. Step 21: ActionAudio Narration 1 5 3 2 4 Description of the action Show serum screen of Rh- woman Show Rh- woman. Show a needle drawing blood from her. Show blood being transferred into different test tubes. Rh- mother is tested for presence of antibodies to Rh antigen at various intervals during pregnancy. A rise in the titre of these antibodies indicates that she has been exposed to Rh antigen and is producing antibodies against them.

10. Step 2: ActionAudio Narration 1 5 3 2 4 Description of the action Show fetal blood analysis Show movement of ultrasound device on the mother’s abdomen. Show a needle being inserted into her abdomen to reach the fetus, as shown in figure. Fetal blood sampling allows for determination of maternal IgG on the surface of fetal RBCs. Coombs test is employed for detection. NAME OF PROCEDURE: Percutaneous Umbilical Cord Blood Sampling (PUBS)‏ Umbilical Cord

11. Animation design Please see the design template provided in the next slide. This is a sample template, and you are free to change as per your design requirements. Try and recreate the sections/subsections as shown in the template. 1 5 2 4 3

13. Interactivity and Boundary limits In this section, you will add the ‘Interactivity’ options to the animation. Use the template in the next slide to give the details. Insert the image of the step/s (explained earlier in the Section 3) in the box, and provide the details in the table below. The details of Interactivity could be: Types: Drop down, Slider bar, Data inputs etc. Options: Select one, Multiple selections etc Boundary Limits: Values of the parameters, which won’t show results after a particular point Results: Explain the effect of the interaction in this column Add more slides if necessary 1 2 5 3 4

14. Interactivity option 3:Step No: Boundary/limits Interacativity Type Options Results 1 2 5 3 4 Which of the following test is specifically employed to diagnose erythroblastosis fetalis? Choosing the correct option box The correct answer box turns green on clicking. The incorrect answer box turns red on clicking. Box B has the correct answer. Boxes A, C and D have incorrect answers. D. Tuberculin Skin Test C. VDRL A. Glucose Tolerance Test B. Coomb’s Test

15. Links for further reading Reference websites: www.nlm.nih.gov http://en.wikipedia.org www.marchofdimes.com www.mercksource.com Books: Textbook of Immunology, Kuby Textbook of Medical Physiology, Guyton Research papers: