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Date of download: 6/2/2016 Copyright © 2016 SPIE. All rights reserved. Experimental setup for angular and spectrally resolved scattering microscopy. The.

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Presentation on theme: "Date of download: 6/2/2016 Copyright © 2016 SPIE. All rights reserved. Experimental setup for angular and spectrally resolved scattering microscopy. The."— Presentation transcript:

1 Date of download: 6/2/2016 Copyright © 2016 SPIE. All rights reserved. Experimental setup for angular and spectrally resolved scattering microscopy. The scattered light is represented by solid orange lines. In the spectral mode path (S; dashed yellow lines), the scattered light is focused onto a fiber. In the angular mode path (A; dashed red lines), the back focal plane is focused onto a CCD camera. Figure Legend: From: Angular and spectrally resolved investigation of single particles by darkfield scattering microscopy J. Biomed. Opt. 2012;17(11):117006-117006. doi:10.1117/1.JBO.17.11.117006

2 Date of download: 6/2/2016 Copyright © 2016 SPIE. All rights reserved. Angular distribution of the scattered intensity for sample PS4-2#1 at a particular wavelength (λ=620 nm). (a) Fourier plane measurement IE,620(u,v). (b) Best fit theory IT,620(u,v,D620). (c) Experimental IE,620( ϑ,φ) and (d) theoretical IT,620( ϑ,φ,D620) scattering pattern versus scattering angles. (e) Correlation function C620(D) with the maximum at D620=4.125 μm. Figure Legend: From: Angular and spectrally resolved investigation of single particles by darkfield scattering microscopy J. Biomed. Opt. 2012;17(11):117006-117006. doi:10.1117/1.JBO.17.11.117006

3 Date of download: 6/2/2016 Copyright © 2016 SPIE. All rights reserved. Scatter spectrum IE(λ) of a single bead PS4-2#1 (light blue line) and theoretical spectrum IE(λ,Dspec) for a sphere diameter Dspec=4.128 μm (dark blue line). Figure Legend: From: Angular and spectrally resolved investigation of single particles by darkfield scattering microscopy J. Biomed. Opt. 2012;17(11):117006-117006. doi:10.1117/1.JBO.17.11.117006

4 Date of download: 6/2/2016 Copyright © 2016 SPIE. All rights reserved. (a) Statistical analysis of the estimated diameter values Dλ for 5 samples of PS4-2 and (b) 5 samples of PS2-8. Diameters determined from spectrally resolved measurements Dspec are plotted as red asterisks. Figure Legend: From: Angular and spectrally resolved investigation of single particles by darkfield scattering microscopy J. Biomed. Opt. 2012;17(11):117006-117006. doi:10.1117/1.JBO.17.11.117006

5 Date of download: 6/2/2016 Copyright © 2016 SPIE. All rights reserved. Comparison of experiment and theory for sample PS2-8#5. (a) Experimental IE,530( ϑ,φ) and (b) theoretical IT,530( ϑ,φ,D530) scattering pattern versus scattering angles with D530=2.742 μm. (c) Measured spectrum IE(λ) (light blue line) and theoretical spectrum IE(λ,Dspec) with Dspec=2.739 μm (dark blue line). Figure Legend: From: Angular and spectrally resolved investigation of single particles by darkfield scattering microscopy J. Biomed. Opt. 2012;17(11):117006-117006. doi:10.1117/1.JBO.17.11.117006


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