1. Henrietta Lacks – Part 1
Born August 1, 1920
Died October 4, 1951

2. Henrietta Pheasant Lacks and
David (Day) Lacks (Married on
April 10, 1941)

3. David (Sonny) Lacks Jr. (Then and Now)
3rd son ?
And
Joe Lacks 5th child now called
Zakariyya Bari Abdul Rahman
Lawrence Lack
1st born child

4. Clover, Virginia (Henrietta’s Home Town)

5. Hennie’s Home-Place
and
Burial Place in Clover

6. Day and all of Henrietta’s
Children born here except
Deborah

7. Can one person make a difference!
This is a childhood picture of Deborah Lacks, age 13 (she is Henrietta’s youngest
daughter). Henrietta got pregnant with her first daughter, Elsie, about this same
age. Deborah was fending off her cousin Galen around this age.

8. Farming in Clover

9. Henrietta and day continued to farm the land
Their slave ancestors had.

10. Henrietta married her cousin Day, who she had grown up with in Grandpa Tommy’s house
Hennie said “Day would bring home Bad
Blood and it would hurt to be with that man”.

11. “Bad Blood” = Syphilus

12. Syphilis
Syphilis is caused by a spirilla bacterium. Before 1955 syphilis was treated with mercury.
Today syphilis is treatable with penicillium.

13. “Bad Blood” = Gonorrhoeae
Gonorrhoea is a sexually transmitted
disease caused by a bacterium.
Henrietta had acute Gonorrhea superimposed on radiation reaction!

14. The Worst “Bad Blood” of all, HPV (human pallinoma virus) which was unknown until 1970’s

15. HPV on the Cervix = Cervial Cancer

17. Land of opportunity ! Day, Henreitta and children move to Maryland
Henrietta had never traveled far
from home in Clover, Virginia.
Above: Housing for workers at Sparrows
Point, Maryland.
Outbreak of WWII created jobs in Maryland’s
shipyards especially Sparrows Point!

18. Work was dirty, hot, and
Tiring but the pay was good
(still less for black workers
than white workers).
All workers were being
exposed to coal dust, asbestos,
and other pollutants on a
daily basis.

19. Why was enter Johns Hopkins like entering A foreign country for Henrietta Lacks?

20. John Hopkins Hospital
Baltimore, Maryland

21. Johns Hopkins Hospital
Segregated Ward – Where Henrietta
recovered from her first radium
treatment and died 8 months
later.
Early Operation at Johns Hopkins

22. Elsie’s Fate-
She was placed in Crownsville
State Hospital, Baltimore,
an institution for the insane
because Henrietta was so weak
she could not care for her. Elsie
will die at Crownsville.

24. Henrietta told Margaret and Sadie (her
cousins) “I got a knot inside me”

28. P
Pathology – Biopsy –

30. Dysplasia –
Carcinoma in situ -

32. Dr. Gey’s wife Margaret Gey (a operating room
nurse) worked with Dr. Gey for 30 years trying
to develop a human cell line in culture.
Margaret’s
Sterile
Technique
Made the
Tissue lab a
Success.
Dr. George Gey – grew first human
cell line in culture known as HeLa cells.

33. Sterile Technique and Culturing Cells
(dark brown
represents
sterile
nutrient
agar)
Use bleach
to wipe down
all surfaces.
Remember
bacteria travel
on dust particles,
slightly open
petric dish lid.
2. Inoculate (heat)
loop before
applying cells.
4. Use
standard
streaking
method to
apply cells.

34. Sterile environment is a MUST in order not to contaminate cell cultures

36. Huge Medical Debate
In 1951 Cervical Cancer was divided into 2 different types:
1. Carcinoma in situ (cancer in its original
place) or non-invasive carcinoma.
2. Invasive carcinoma
Doctors knew the invasive carcinoma was deadly so they treated it aggressively by hysteretomy.
However, they believed wrongly that carcinoma in situ was nonlife threatening and didn’t wrong about it.

38. Carcinoma in situ Treatment #1- Radium
Doctor Sews
Brack Plaques
(Radium) to the
Cancer tumor
Located on the cervix of patient.
Radium

39. 2nd Treatment for Carcinoma in situ Radiation

40. Invasive Carcinoma Treatment-Hysteretomy

41. PAP Smear (Preventive Care)

42. Cancer is Mitosis Gone Crazy
HeLa Cell dividing

43. 1. Why do cancer cells (tumors) need a large blood supply?
Because body cells
Continue to divide
Uncontrollably, they
Pile up and form a
Tumor.
1. Why do cancer cells (tumors) need a large blood supply?
2. How do cancer cells move around in the body?

44. Mediatized Cancer Cells

45. Cancer Causing Mutations

46. Some viruses cause mutations resulting in Cancer
For example, HPV!

47. Death of Henrietta Lacks
Henrietta’s Death Certificate
Notice cause of death????

48. Discoveries Aided By HeLa Cells
Salk lab technicians working on
ROLLER DRUMS containing tube
cultures where polio virus was
grown.

49. Aided in Making Polio Vaccine
Iron Lung Used to Keep Polio Patients Breathing

50. Jonas Salk used Hela
Cells to test the
Effects of his polio
Vaccine on human
Cells.

51. Where would HeLa cells come into the picture?

52. Thanks to
HeLa Cells!

53. Maybe there should be a stamp for Henrietta’s Contribution ?????

55. Hennie’s Family Dealing With News
Deborah finally gets to see her
Mothers immortal cells.
Recognition for Henrietta makes
her family proud.

56. Glossary Aneuploid – an abnormal number of chromosomes
Aseptic – system that prevents infection by being free of microorganisms
Biosynthesis – the formation of chemical compounds, derived from living organisms
Centrifugation - an instrument that rotates at high speed to separate substances of different concentrations
Continuous/Immortalised Cell Cultures – cells that are derived from transformed cells that are generally epithelial in origin
Diploid – a cell with double number of chromosomes (total number of chromosomes in body cells)
Growth Media – a liquid or gel designed to support the growth of microorganisms or cells
Haploid – a cell with a complete set (half the number of a diploid cell) of chromosomes
Heteroploid – a cell with an abnormal number of chromosomes
In Vitro - where a procedure occurs within a controlled external environment rather than in the living organism
Primary Cultures – cells that are derived directly from a host’s tissue or organ
Sanitation – the process and application of measures to ensure the prevention of disease and contamination
Subculture – a cell culture made by transferring cells from a previous culture to a fresh growth medium

57. Present Day 2013

58. Other Cell Lines

61. In this image, the ghost of Henrietta is metaphorically being 'drained', by doctors, as the intravenous tubes continue forward in an endless spiral of taking, taking, taking.

62. So What’s Next Would you like your hamburger Grown in a petric dish?
Well you are looking at the first
One above!!!!