1. SYBR Green I Chemistry Detects all double stranded DNA
Low cost alternative
can easily be upgraded to TaqMan assay
Dissociation curves needed
PCR, Light

2. Smarter chemistry: The TaqMan™ probe3'

3. TaqMan® chemistry R Q TaqMan® Probe Forward Primer Reverse Primer 5’ p3’ 5’ 5’ 3’ 5’
Reverse
Primer

4. TaqMan® chemistry R Q 5’ p 3’ 5’ 5’ 3’ 5’
Displacement and cleavage

5. TaqMan® chemistry R Q 5’ p 3’ 5’ 5’ 3’ 5’
Displacement and cleavage

6. TaqMan® chemistry Polymerization Strand displacement Cleavage
R = Reporter
Forward Q
Q = Quencher
Primer R
Probe 5' p 3' 3' 5' 5' 3' 5'
Reverse
Primer
Strand displacement R Q 5' p 3' 3' 5' 5' 3' 5'
Cleavage R Q 5' p 3' 3' 5' 5' 3' 5'
Polymerization completed R Q p 3' 5' 3' 5' 5' 3' 5'

7. Amplification plot

8. Real-time versus end-point

9. Closed Tube Detection Fluorescence detection through tube
Detection during amplification
No contamination
No further steps needed
Automated Quantitation after run