1. SGPP Crystal Growth Status 1/9/04

2. Crystal Status 31 proteins received since 12/1/03 2 SelenoMet received, 1 was a remake 3 new SulfurMet proteins sent downstream for mounting –Lmaj006129AAA –Lmaj006828AAA –Lmaj01134AAC-Chunk

3. Chunk Protein LMAJ01134AAC was received on 12/4/03 Buffalo got nice big hits at 1 week review and Seattle set it up immediately We got big harvestable crystals in 2 days. Crystals went downstream to the Hol group on 1/7/04 SelenoMet protein has been requested

4. Lmaj01134AAC

5. When to request SelenoMet Protein? Lmaj006129AAA was received on 10/1/03 Buffalo found 25 marginal hits at 11/3/03 –Hits were based on previous “limits” –5 marginal hits based on current standards Buffalo follow-up was set up in Seattle on 11/6/03 Plates were checked weekly and no hits or indication of crystal growth On 1/7/04 nice form, medium (90um x 70 um) crystals were detected and passed downstream to the Merritt group

7. Purity SDS GELNATIVE GEL 5 ug 30 ug 5 ug

8. Seattle crystals, almost 2 months later

9. Question? Should we ask for SelenoMet as soon as Buffalo reports hits? Should we base SelenoMet request on purity and quality of Buffalo hits? Should we wait until the Seattle Growth Lab has reproduced the hits?