1. Note: Glacier is launched unpowered and hardmounted in EXPRESS Rack 6 on Flight ULF2. For the following three weeks after activation or transfer, two 2-hour command/downlink windows per week are required preferably on Monday and Thursday at 9am CST. After one month of on-orbit ops, one window a week preferably on Thursday is required. The ULF2 unit should be activated approximately 2 weeks prior to the first sample insertion. Currently, there are no sample insertions until the transfer of returning items from MELFI to Glacier (DCB-15A-PRE SMP XFR). Cold Stowage will submit a comment if samples are to be inserted into Glacier prior to this transfer. PD must command to disable/enable the cold volume fan around any door openings for the ULF2 unit to limit ice buildup. 15A unit software will alleviate requirement for PD commanding to fan during door openings. Health & Status (H&S) data (1 Hz) is downlinked via PL MDM. As long as the Glacier ground team can receive H&S data, crew is not required to perform additional status checks after activation. If no Ku-band coverage can be obtained in a 24-hour period, two checks separated by 8-12 hours are required for that day. Power interruptions must be limited to 30 minutes when samples are inside Glacier to prevent degradation of scientific results. Historical photos/video are required of crew working at the Glacier for each unit transferred to ISS. Prior to the 15A software load, all crew interface with Glacier will be via the front panel. Glacier Inc 18 GLACIER-STATUS-CHECK Crew checks status of Glacier after initial activation. After activation, status checks are only required when Ku-band AOS cannot be obtained in a 24-hour period. GLACIER-SW-LOAD Crew loads the Glacier payload application software onto the ELC from CD. GLACIER-HW-PWR Unattended activity to track Glacier power and thermal resources. -95C (160W) GLACIER-VIDEO-SETUP Crew sets up camcorder to obtain video of the setup. ~2 weeks ULF2UNDOCKULF2UNDOCK ULF2DOCKULF2DOCK 15ADOCK15ADOCK PRO-GLACIER ACT-CMD PRO sends commands to configure the RFCA and activate Glacier. Higher Active Logic (HAL) will issue PL MDM startup notification. Hardware Dry and Cool :10 GLACIER-HW-SETUP Crew connects cables, configures Glacier switches, installs desiccant canister, and places desiccant packs inside Glacier. 1:00 1:30 ASAP :5:25 15AUNDOCK15AUNDOCK Glacier Flight 15A Rack Power Down/Up ASAP Glacier Flight ULF2 DCB-15A-PRE SMP XFR Two crewmembers transfer returning samples, Rad Gene & LOH and HRP Nutrition/Bisp, from MELFI to Glacier. :30 Prior to dock GLACIER-DNLK-CMD PD commands to check status of Glacier and downlink data. Approximately 1 hour of Ku- band AOS is required for every 2 hours. Intermittent coverage is acceptable. GLACIER-PD-CMD PD commands to disable/enable Glacier fan around door opening. Time Critical: Ku- and S-band required for commanding. :20 GLACIER-HISD-VIDEO Crew takes video of another crewmember performing an activity at Glacier. GLACIER-HISD-PHOTO Crew takes photos of another crewmember performing an activity at Glacier. :15 2:00 PD Commanding PRO Commanding Crew Additional Activities Time Critical Unattended Activities 09/22/08

2. Note: Two dry cycles are noted in URC, but Glacier continues drying from initial command after activation to cool down. At least 24 hours are required for Glacier to dry. Glacier team will check humidity during the second command window and initiate cool down when the unit is dry. For planning purposes, cool down can start at the beginning of the second command window. Glacier requires at least 24 hours to cool and fully stabilize. Glacier Hardware Dry and Cool PD Commanding Crew Additional Activities Time Critical 09/22/08 Unattended Activities 24:00 16:00-18:00 No earlier than 24 hours from activation 16:00-18:00 GLACIER-DSC PACK-RMV Crew removes NGTC desiccant packs from Glacier interior. Time Critical: S-band required for crew call-down at completion. Glacier Activation GLACIER-HW-DRY Unattended Glacier drying. GLACIER-DNLK-CMD PD commands to initiate Glacier drying and downlink data. GLACIER-DNLK-CMD PD commands to initiate Glacier cool down and downlink data. 2:00 Day after completion of cool down GLACIER-DNLK-CMD PD commands to verify Glacier temperature is stable and to downlink data. :10 At least 24 hours GLACIER-HW-COOLDN Unattended Glacier cool down. Cool down requires additional 55W.

3. GLACIER-HW-COOLDN Unattended Glacier cool down. Cool down requires additional 55W. 09/22/08 Note: Glacier can be powered down/up via ground commanding since the circuit breaker can be left closed. Prior to rack powerdown, the Glacier team will command shutdown followed by PRO commanding to disable power, comm, and water. The PRO has authority to command Glacier power off/on when the Glacier ground team is not available. After rack powerup, PRO will command to enable power, comm, and water. No additional ground commanding from the Glacier team is required for powerup. Apply a 1:1 ratio for cool down duration, e.g., two hours of cool down for a two-hour power off event. Glacier Rack Power Down/Up 1:30 Rack Power Down/Up 1:30 PD Commanding PRO Commanding Time Critical PRO-GLACIER DACT-CMD PRO sends commands to configure the RFCA and deactivate Glacier. Higher Active Logic (HAL) will issue PL MDM shutdown notification. PRO-GLACIER ACT-CMD PRO sends commands to configure the RFCA and activate Glacier. Higher Active Logic (HAL) will issue PL MDM startup notification. GLACIER-HW-PWR Unattended activity to track Glacier power and thermal resources. GLACIER-PD-CMD PD commands to shut down Glacier prior to removal of power by PRO. Time Critical: Ku- and S-band required for commanding. Unattended Activities :15

4. Glacier As Needed Activities Note: Glacier units are planned for return before one year of on-orbit ops. Glacier remains powered during filter cleaning. PD must command to disable/enable the cold volume fan around any door openings for the ULF2 unit to limit ice buildup. 15A unit software will alleviate requirement for PD commanding to fan during door openings. Transfer prep only required if no other sample transfer activities to/from Glacier are planned prior to return. For most stages, returning items in MELFI are transferred to Glacier just prior to Shuttle docking. GLACIER-DSC CAN-RPLC Crew replaces desiccant canister. Only required after one year of on- board ops. GLACIER-FILTER-CLEAN Crew cleans inlet filter on the back panel. Only required when AAA used as primary heat rejection for 90 continuous days. GLACIER-SMPL-REMOVE Crew removes sample from Glacier. GLACIER-PD-CMD PD commands to disable/enable Glacier fan around door opening. ULF2 unit only. Time Critical: Ku- and S-band required for commanding. GLACIER-SMPL-INSERT Crew inserts sample into Glacier. GLACIER-XFR-PREP Crew performs quick cursory of Glacier contents and reports status to ground. Time Critical: S-band for voice communication. PD Commanding Crew Time Critical :15:10 :30 :15 :10 09/22/08