1. + P4 membrane coating process {Cutie, 1997 #1}

2. +

3. +

4. + Conversion vs. time ‘dead’ time Inhibitor Retarder Three sets of data illustrating the overall reaction

5. +

6. + Crosslinking acrylates TMPTA – trimethylol propane triacrylate, 15625-89-5 NMBA – N,N’-methylene bis acrylate, NMBA will connect two polymer chain segments; TMPTA will connect three polymer chain segments

7. +

8. +

9. + Commercial PVDF membranes http://www.emdmillipore.com/US/en/ microfiltration- membranes/FqCb.qB.7ZkAAAFBDQZlv zJE,nav?cid=BI-XX-BSP-P-MICR-ASP0- B343-1024 http://www.emdmillipore.com/US/en/ microfiltration- membranes/FqCb.qB.7ZkAAAFBDQZlv zJE,nav?cid=BI-XX-BSP-P-MICR-ASP0- B343-1024 Millipore Durapore membranes Durapore® Membrane Filters Material: PVDF (Polyvinylidene fluoride) Features: Low Protein Binding Applications: Particulate removal. Compatible with aqueous solutions and some organic solvents.

10. + Specifications General Specifications Color: white Surface: plain Thickness: 125 µm Sterilization: by autoclave (121 °C at 1 bar), EO or gamma Operating temperature: 85 °C max. Bacterial endotoxins: 0.5 EU/mL Gravimetric extractables: < 0.5% Type = DVPP; 0.65 micron pores;, hydrophilic; > 78 mL water/min/cm 2

11. +

12. + Coating recipe

13. +

14. + Recipe components

15. +

16. +

17. +

18. +