1. shCon shNaa10p GAPDH MCL1 EPHB4 RPS6KA5 SMAD1 BFAR EIF4B CYR61 STAT5B NFkbil2 VEGFC TNFRSF1A CyclinD Supplementary Figure S1 RKO cells A B siCon siNaa10p-395siNaa10p-464siNaa10p-649 RKO cells Relative mRNA levels

2. ShConshNaa10pshConshNaa10pshConshNaa10p inputIgGanti-p65 MCL1 promoter ChIP MCL1 promoter inputIgGanti-Naa10p MCL1 promoter siConsip65siConsip65siConsip65 inputIgGanti-Naa10p ChIP A D B Supplementary Figure S2 C MCL1 Probe Naa10p antibody RKO nuclear extract MCL1 probe (200X Cold) IgG +++ － + －－－ －－ + － + － + －－ +++ ++++ －－－ MCL1 probe (400X Cold) －－－ + －－－－－ Free probe Band shift Supershift q C H I P o f M C L 1 p r o m o t e r ( F o l d c h a n g e o f I g G ) IgGNaa10p1 0 5 10 15 IP:

3. A C B Supplementary Figure S3 Relative Naa10p Expression Normal (n=5) Tumor (n=43) Relative Naa10pmRNA Expression Correlation between Naa10pand p65 in lung cancer tissues 5 6 7 8 9 3579 r=0.277 p=0.056 Naa10p p 6 5 Correlation between Naa10pand MCL1 in lung cancer tissues 2 4 6 8 10 2468 r=0.467 p<0.001 Naa10p M C L 1 Relative MCL1 mRNA Expression Relative p65mRNA Expression Relative MCL1 Expression Relative p65 Expression Correlation between MCL1and p65 in lung cancer tissues 5 6 7 8 9 246810 r=0.535 p<0.001 MCL1 p 6 5