1. Purple Non Sulfur Bacteria
Hopkins Microbiology Course
Ann Lesnefsky

2. Metabolism of PNS Bacteria
Organic acid electron donor
Photosynthesis for energy generation
Bacterial chlorophyll b
Absorbs light in the infrared range
Currently PNS bacteria are being studied for H2 production

3. Course Enrichment Method for PNS
Plated samples from the slough on succinate and ethanol plates, incubated anaerobically with light
Course’s media and Holly’s media
Restreak single colonies for isolation

4. TA Enrichment Method for PNS
TA’s enriched for PNS by filtering
Filter both water above mat and slurry of mat material
Place filter face down on plate
Flip and all allow PNS bacteria to grow on filter

5. Many Some Few Carbon Source Enrichment Method Succinate Ethanol
(# of colonies before restreak)
Ethanol
No filter
Course 43 6
Holly
Filter
Many
Some
Few
None

6. Microscopy of PNS Colonies isolated from 5 different plates
4 plates from strawberry
1 from Kerby Park
All looked like stubby rods

7. Recommendations
Next year use the filter method.

8. Questions?