4nutritional classification: metabolic strategy aerobic respirationiron oxidizersE source to oxidize e-light/chemicallight energy phototrophchemical energy chemotrophcarbon sourceorg./inorganicphotoheterotrophphotoautotrophchemoheterotrophchemoautotrophelectron sourceorganic/inorganicphotoorgano-heterotrophphotolitho-chemoorgano-inorganicautotrophoxygenother inorganicelectron acceptor org./inorganicfermentation butanediol mixed acid lactic acid alcoholrespirationchemolitho-anoxygenic photosynthesisanaerobic respirationsulfur oxidizersoxygenic photosynthesise- acceptor oxygen/otherPurple non-Sulfur bacteriaPSB, GSB, GNSBCyanobacteria (plants)We categorize based on carbon, energy, and electron sourceNSP bacteria grow photoheterotrophically using malate or succinate as e-, C and light
13Chapter Five Learning Objectives Discuss redox reactions in biological systems.Identify the redox partners in aerobic and anaerobic respiration and oxygenic and anoxygenic photosynthesis.Correctly identify the carbon, energy and electron source for an organism when given its nutritional classification (e.g., chemoorganoheterotroph).How is ATP generated in both substrate level and oxidative phosphorylation?Why is it so important that the electron transport chain is housed in a lipid bilayer membrane? Why is a terminal electron acceptor so important?What happens in a microorganism if the terminal electron acceptor of the ETC is not available? What molecules build up? What is done with these molecules?Discuss the major differences between respiration and fermentation. What are the four basic kinds of fermentation?
14autotrophy: chemosynthesis chemo-: conversion of chemical E ATPiron oxidationsulfur oxidation-synthesis: carbon fixation (CO2 organic molecule)ADP + PETCATPNAD NADHcarbonfixation2 H+2Fe2+2Fe3+heterotrophy2 H+ADP + PETCATPNAD NADHcarbonfixationH2SSO42-heterotrophy
15chemosynthesis: iron oxidation Thiobacillus ferrooxidanschemolithoautotrophyenergy = Fe2+ Fe3+electron = samecarbon = CO2 CH2O
23metabolic diversity: the non-sulfur purple bacteria chemoheterotrophic growthaerobic respirationfermentationphotoautotrophic growthanaerobic, anoxygenic photosynthesis H2 for e- & CO2 for Cphotoheterotrophic growthanaerobic, anoxygenic photosynthesis C6H6O4 (succinate) for both
24chapter 5 learning objectives How is ATP generated in chemosynthesis, photosynthesis and respiration? How is the process different for each and how is it the same?Discuss the redox partners of sulfur and iron oxidizing bacteria.How do non-cyclic and cyclic photosynthesis differ? How does each produce ATP and NADPH/NADH? What is each used for?How is carbon fixed during chemosynthesis and photosynthesis? How is the process similar and how is it different?How do amphibolism, catabolism and anabolism relate to growth and repair in cells?