1. STRUCTURE AND CIRCUITS OF THE BASAL GANGLIA Rastislav Druga Inst. of Anatomy, 2nd Medical Faculty

2. Basal ganglia Nucleus caudatus, putamen, globus pallidus (pallidum externum, pallidum internum), amygdala, claustrum Nucleus caudatus, putamen, globus pallidus (pallidum externum, pallidum internum), amygdala, claustrum Nucleus caudatus + putamen = striatum Nucleus caudatus + putamen = striatum Putamen+ globus pallidus (pallidum externum, pallidum internum) = nucleus lentiformis Putamen+ globus pallidus (pallidum externum, pallidum internum) = nucleus lentiformis Amygdala ( limbic system) Amygdala ( limbic system) Claustrum Claustrum

3. Topography of the basal ganglia

4. Dorsal striatum, Ventral striatum (nc. Accumbens)

5. Dorsal pallidum, Ventral pallidum

8. 1 – 3 caudate nc. 4 – putamen 5 – globus pallidus 6 - amygdala

9. S

10. A-projekční neurony, GABAergní, 95 % B – cholinergní neurony, 1 %

11. Medium spiny neurons, projecting neurons (globus pallidus, substantia nigra), GABAergic, 95 %

13. NOS-positive neurons

14. STRIATUM - afferent connections cortex, thalamus, amygdala, substantia nigra (p. compacta, Dopamin)

17. Anterograde intraaxonal transport Cortex – striatum PM + M I - putamen

18. RetrogradeIntraaxonalTransport Assoc. Cortex- Nc. caudatus

20. Assoc. Cortex – nc. caudatus

23. Amygdalo – striatal projections Amygdala- Projects to the Ventral striatum (Nc. Accumbens)

24. Cortico – striatal projections - summary Cortico – striatal projections - summary

25. Distribution of corticostriatal neurons in cortical layers Large dots – striatum Small dots - thalamus

26. Nigro-striatal projections Dopaminergic

29. Raphe nuclei – Striatum /pallidum

30. THALAMOSTRIATAL PROJECTION

31. THALAMIC NEURONS PROJECTING TO THE STRIATUM (CAUDATE NUCLEUS)

32. STRIATUM - efferent connections globus pallidus (GPe,GPi), subst. nigra (p. reticulata) Putamen – globus pallidus Caudate nucleus – subst. nigra (pars reticulata)

34. Striato-nigral projections Putamen – s. Nigra weak proj.

35. Caudate nc. – subst. Nigra Strong projection

36. GLOBUS PALLIDUS afferent connections: Striatum ( Pe, Pi), Nc.subthalamicus (Pe) efferent connnections : Pallidum externum - Nc. Subthalamicus Pallidum internum - Thalamus (VA, IL,Hb), Reticular formation

39. Circuit of the basal ganglia

41. CIRCUIT OF THE BASAL GANGLIA (Nauta – Mehler 1966) CORTEX- STRIATUM – PALLIDUM – THALAMUS - CORTEX

42. Limbic circuit Prefrontal circuits

43. CIRCUIT OF BASAL GANGLIA (Nauta –Mehler circuit, 1966) Neocortex – striatum – globus pallidus – thalamus (VA) – neocortex – cortical projections to the brain stem and to the spinal cord

44. Direct and indirect pathways of basal ganglia Direct pathway : cortex – (+) striatum – (-) pallidum internum/subst. nigra – (-) thalamus – (+) cortex ( increases the activity of the thalamus and the excitation of cerebral cortex = increased motor activity) Direct pathway : cortex – (+) striatum – (-) pallidum internum/subst. nigra – (-) thalamus – (+) cortex ( increases the activity of the thalamus and the excitation of cerebral cortex = increased motor activity) Indirect pathway : cortex – (+) striatum – Indirect pathway : cortex – (+) striatum – (-) pallidum externum – (-) subthalamic nc. – (+) pallidum internum – (-)thalamus – (+) cortex (decreases activity of the thalamus and the excitation of cerebral cortex = decreased motor activity) (-) pallidum externum – (-) subthalamic nc. – (+) pallidum internum – (-)thalamus – (+) cortex (decreases activity of the thalamus and the excitation of cerebral cortex = decreased motor activity)

45. Behavioral functions of the basal ganglia I. Hypokinesia Hypokinesia Akinesia – impairment in the iniciation of movement Akinesia – impairment in the iniciation of movement Bradykinesia – reduction in velocity and amplitude Bradykinesia – reduction in velocity and amplitude Parkinson disease (tremor at rest, flexed posture, paucity of limb and facial movements) Parkinson disease (tremor at rest, flexed posture, paucity of limb and facial movements) Decrease in production of Dopamine Decrease in production of Dopamine Loss of dopaminergic neurons within substantia nigra (pars compacta). Loss of dopaminergic neurons within substantia nigra (pars compacta). L-Dopa will cross BBB (after amination is converted to Dopamin L-Dopa will cross BBB (after amination is converted to Dopamin

46. Behavioral functions of the basal ganglia II. Hyperkinesia Hyperkinesia Choreiform movements – irregular dancelike movements of the limbs and in facial muscles – loss of striatal medium spiny neurons, decrease in the size of the striatum, gliosis – Huntington disease (major affective psychiatric disorders) Choreiform movements – irregular dancelike movements of the limbs and in facial muscles – loss of striatal medium spiny neurons, decrease in the size of the striatum, gliosis – Huntington disease (major affective psychiatric disorders) Hemiballism – uncontrolled (dangerous) flingting movements of limbs – vascular lesion in the subthalamic nucleus Hemiballism – uncontrolled (dangerous) flingting movements of limbs – vascular lesion in the subthalamic nucleus

47. LESIONS AND STIMULATIONS OF THE STRIATUM (an inhibitory structure) LESIONS – hyperactivity LESIONS – hyperactivity drive to run forward, regardless of obstacles (without to avoid the obstacle) drive to run forward, regardless of obstacles (without to avoid the obstacle) stereotyped approaching and following of persons, objects stereotyped approaching and following of persons, objects Tremor Tremor Choreic - like movements Choreic - like movements STIMULATIONS – STIMULATIONS – Arrest of voluntary movements and speech Arrest of voluntary movements and speech Short confussion and amnesia Short confussion and amnesia Sleep – like effect Sleep – like effect Rejecting of food Rejecting of food Inhibition of agressive behavior Inhibition of agressive behavior