1. Histology Lab 1 Introduction and Epithelium Jun Zhou （周俊）, Ph.D & M.D School of Medicine,Zhejiang University

2. Note: Please come lab on time. Find your seat according your lab number.

3. Your final score is composed of four parts 1) Attendance and picture drawing:10% 2) Quiz: 15% (each quiz 5%) 3) Final lab test: 25% 4) Final written examination: 50% How to pass this course: Final score>60 and Final written exam>50

4. Lab facilities: 1 Light electron microscope with computer 1 case of slides 1 atlas http://m-learning.zju.edu.cn 1.Students share these facilities 2.Do not take them out the laboratory with you. 3.Put them where they belong to when lab is over. 4.Do not left any rubbish after you. 5.No food in the lab. 6.Lecture and lab slides online: m-learning.zju.edu.cn 2015 Histology and Embryology

5. Contents of Lab 1: 1.Introduction 2.Epithelium 1)Simple squamous epi (small intestine,stomach) 2)Simple cuboidal epi.(thyroid) 3)Simple columnar epi.(small intestine)(drawing) 4)Pseudostratified ciliated columnar epi.(trachea) 5)Stratified squamous epi.(esophagus) 6) Transitional epi.(bladder) 3.Lab report (content of lab, drawing)

7. Tissue Processing 1.Fixation: 10% Formalin 2.Dehydration:graded concentrated ethyl alcohol 3.Clearing:xylene,infiltrate with paraffin solvent. Loss of fat due to ‘clearing’

8. Tissue Processing 4.Embedding 5.Sectioning

9. 6.Staining

10. Common Stains: Hematoxylin & Eosin Ross, 5 th ed, fig 1.1

11. Common Stains Wright’s stain Hematoxylin and eosin ("H&E")

12. Relative Sizes in Histology Standard Units of Measure

13. micrometer: 10 -6 meter nanometer: 10 -9 meter Relative Sizes in Histology Standard Units of Measure

14. Changes in apparent shape, due to orientation of sections Ross, 5 th ed, fig 1.11

15. Changes in apparent shape, due to orientation of sections Netter/Ciba Junqueira fig 1-30

16. Epithelium No blood vessles, nourishment is by diffusion Epithelium is innervated polarization Trachea Epithelium

17. Classification of Epithelia Number of cells 1. Simple 2. Pseudostratified 3. Stratified Shape of surface cells 1. Squamous 2. Cuboidal 3. Columnar 4. Transitional

18. Stomach (NO 25): mesothelium HE ×400 Simple squamous epi.

19. Small intestine (NO 1): endothelium HE × 400

20. Thyroid (NO 35): HE × 100

21. Thyroid: HE × 400 Simple cuboidal epi.

22. Small intestine (NO 1): villus HE × 400 (Drawing ) Simple columnar epi., Goblet cells, striated border (microvilli)

23. Trachea Pseudostratified ciliated columnar epi

24. Trachea: HE × 400 Pseudostratified ciliated columnar epi.

25. Esophagus (No.2) Stratified squamous epi.

26. Esophagus: HE × 100

27. Transitional epithelium distendedcontracted Bladder

28. Bladder (NO33): HE × 40

29. Bladder: HE × 100 Transitional epi.

30. Bladder: HE × 400 Transitional epi.

31. The end!