1. BIOLOGY CONCEPTS & CONNECTIONS Fourth Edition Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Neil A. Campbell Jane B. Reece Lawrence G. Mitchell Martha R. Taylor From PowerPoint ® Lectures for Biology: Concepts & Connections CHAPTER 8 The Cellular Basis of Reproduction and Inheritance Modules 8.4 – 8.11

2. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings A eukaryotic cell has many more genes than a prokaryotic cell –The genes are grouped into multiple chromosomes, found in the nucleus –The chromosomes of this plant cell are stained dark purple 8.4 The large, complex chromosomes of eukaryotes duplicate with each cell division THE EUKARYOTIC CELL CYCLE AND MITOSIS Figure 8.4A

3. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Chromosomes contain a very long DNA molecule with thousands of genes –Individual chromosomes are only visible during cell division –They are packaged as chromatin

4. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Before a cell starts dividing, the chromosomes are duplicated –This process produces sister chromatids Centromere Sister chromatids Figure 8.4B

5. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings When the cell divides, the sister chromatids separate –Two daughter cells are produced –Each has a complete and identical set of chromosomes Centromere Sister chromatids Figure 8.4C Chromosome duplication Chromosome distribution to daughter cells

6. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings The cell cycle consists of two major phases: –Interphase, where chromosomes duplicate and cell parts are made –The mitotic phase, when cell division occurs 8.5 The cell cycle multiplies cells Figure 8.5

7. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Eukaryotic cell division consists of two stages: –Mitosis –Cytokinesis 8.6 Cell division is a continuum of dynamic changes

8. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings In mitosis, the duplicated chromosomes are distributed into two daughter nuclei –After the chromosomes coil up, a mitotic spindle moves them to the middle of the cell

9. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings INTERPHASEPROPHASE Centrosomes (with centriole pairs) Chromatin NucleolusNuclear envelope Plasma membrane Early mitotic spindle Centrosome Chromosome, consisting of two sister chromatids Fragments of nuclear envelope Kinetochore Spindle microtubules Figure 8.6

10. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings The sister chromatids then separate and move to opposite poles of the cell –The process of cytokinesis divides the cell into two genetically identical cells

11. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings METAPHASETELOPHASE AND CYTOKINESIS Metaphase plate SpindleDaughter chromosomes Cleavage furrow Nucleolus forming Nuclear envelope forming ANAPHASE Figure 8.6 (continued)

12. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings In animals, cytokinesis occurs by cleavage –This process pinches the cell apart 8.7 Cytokinesis differs for plant and animal cells Figure 8.7A Cleavage furrow Contracting ring of microfilaments Daughter cells

13. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings In plants, a membranous cell plate splits the cell in two Vesicles containing cell wall material Cell plate forming Figure 8.7B Cell plateDaughter cells Wall of parent cell Daughter nucleus Cell wallNew cell wall

14. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Most animal cells divide only when stimulated, and others not at all In laboratory cultures, most normal cells divide only when attached to a surface –They are anchorage dependent 8.8 Anchorage, cell density, and chemical growth factors affect cell division

15. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Cells continue dividing until they touch one another –This is called density-dependent inhibition Cells anchor to dish surface and divide. Figure 8.8A When cells have formed a complete single layer, they stop dividing (density-dependent inhibition). If some cells are scraped away, the remaining cells divide to fill the dish with a single layer and then stop (density-dependent inhibition).

16. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Growth factors are proteins secreted by cells that stimulate other cells to divide After forming a single layer, cells have stopped dividing. Figure 8.8B Providing an additional supply of growth factors stimulates further cell division.

17. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Proteins within the cell control the cell cycle –Signals affecting critical checkpoints determine whether the cell will go through a complete cycle and divide 8.9 Growth factors signal the cell cycle control system G 1 checkpoint M checkpoint G 2 checkpoint Control system Figure 8.9A

18. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings The binding of growth factors to specific receptors on the plasma membrane is usually necessary for cell division Growth factor Figure 8.8B Cell cycle control system Plasma membrane Receptor protein Signal transduction pathway G 1 checkpoint Relay proteins

19. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Cancer cells have abnormal cell cycles –They divide excessively and can form abnormal masses called tumors Radiation and chemotherapy are effective as cancer treatments because they interfere with cell division 8.10 Connection: Growing out of control, cancer cells produce malignant tumors

20. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Malignant tumors can invade other tissues and may kill the organism Tumor Figure 8.10 Glandular tissue 123 A tumor grows from a single cancer cell. Cancer cells invade neighboring tissue. Lymph vessels Cancer cells spread through lymph and blood vessels to other parts of the body. Metastasis

21. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings When the cell cycle operates normally, mitotic cell division functions in: –Growth (seen here in an onion root) 8.11 Review of the functions of mitosis: Growth, cell replacement, and asexual reproduction Figure 8.11A

22. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Cell replacement (seen here in skin) Dead cells Figure 8.11B Dividing cells Epidermis, the outer layer of the skin Dermis

23. Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Asexual reproduction (seen here in a hydra) Figure 8.11C