2. While hiking, a student decided to collect and eat berries from the plants he came across on the AT trail. Unfortunately, he became very ill and had to be taken to the hospital with the following symptoms: pain in mouth and throat, severe upset stomach, vomiting, bloody diarrhea.

3.  As a class, we collected eleven plants from the Appalachian trail  We used genetic techniques to find out each of the plants DNA sequences in their MatK region  The MatK region is in the chloroplast genomes of all plants and is often used to build evolutionary trees of plants

4.  Identify three unknown plant samples each  Determine which plant made the student sick Present findings in mini presentation  Look at the relationship among the unknown plant samples  Each INDIVIDUAL does all these steps, except for presenting with partner or group

5. Each student is assigned a number This number corresponds to a plant (there are eleven plants altogether)

6.  Merges biology, computer science and information technology  Aims to find new insights about biological systems  Often applied to molecular data  NEW AND EXCITING FIELD OF BIOLOGY!

7.  Basic Local Alignment Search Tool  Compares nucleotide or amino acid sequences  Looks for areas of similarity and calculates the statistical significance of the matches (http://blast.ncbi.nlm.nih.gov/Blast.cgi)

9. COPY AND PASTE YOUR SEQUENCE HERE

10. Closer the E-value is to zero the better the match Click accession number of first search result

11. Length of geneType of data Description of gene

12. gene information Encoded Protein DNA sequence

13.  Common name  Poisonous? Symptoms  Other interesting information  Prepare 5-minute presentation with partner or group; each person must speak

14.  Go to http://www.uniprot.org/http://www.uniprot.org/ Enter the genus and gene name here

15. Find and click on the accession for your gene and species

16. Click here to get more information about your gene

17.  Find information on the structure and function of protein with Pfam.  When finished with that, begin next exercise.  Copy and paste all 11 sequences into the Clustal Omega site as is, numbers and ‘<‘s’ and all  http://www.ebi.ac.uk/Tools/msa/clustalo/ http://www.ebi.ac.uk/Tools/msa/clustalo/

21. Phylogram: shows common ancestry and branch lengths indicate amount of evolutionary change Cladogram: shows only common ancestry

22. 1. Hemerocallis fulva (orange daylily) 2. Chenopodium album (lambsquarters) 3. Fragaria virginiana (Virginia strawberry) 4. Gleditsia triacanthos (honeylocust) 7. Rumex obtusifolius (bitter dock) 9. *Phytolacca americana (American pokeweed) 10. Vaccinium parvifolium (red huckleberry) 11. Vaccinium pallidum (Blue Ridge blueberry)