1. Agrobacterium-Mediated Transformation of Penicillium marneffei A. Kummasook, C. R. Cooper, Jr., and N. Vanittanakom. 2010. An improved Agrobacterium-mediated transformation system for the functional genetic analysis of Penicillium marneffei. Medical Mycology 48: 1066–1074. Reviewed by: Dr. Cooper SAMPLE TITLE SLIDE

2. SAMPLE SLIDE - 1 Introduction Penicillium marneffei is a significant agent of disease among HIV-infected persons living in Thailand P. marneffei is thermally dimorphic Dr. Cooper’s favorite fungi are P. marneffei and Wangiella dermatitidis

3. SAMPLE SLIDE - 2 Figure 1 from Kummasook et al., 2010. Selection of putative bleomycin-resistant transformants of Penicillium marneffei. Pre- germinated conidia cultivated in the absence of A. tumefaciens served as a control (a). Very little background growth was observed on the selection media (BHA containing 4 μ g/ml bleomycin). After 36 h of co-cultivation, AMT-treated conidia developed on the selection media as individual yeast-like colonies (b), whereas 48 h of co-cultivation generates high background growth on the same medium (c).

4. SAMPLE SLIDE - 3 Source: www.imperial.ac.uk/college.asp?P=6996

5. References SAMPLE SLIDE - 4 Cooper CR, Vanittanakom N. Insights into the pathogenicity of Penicillium marneffei. Future Microbiol 2008; 3: 43 – 55. Supparatpinyo K, Khamwan C, Baosoung V, Nelson KE, Sirisanthana T. Disseminated Penicillium marneffei infection in southeast Asia. Lancet 1994; 344: 110 – 113. Vanittanakom N, Cooper CR, Jr., Fisher MC, Sirisanthana T. Penicillium marneffei infection and recent advances in the epidemiology and molecular biology aspects. Clin Microbiol Rev 2006; 19: 95 – 110.