1. IGP NCRR Capecchi Laboratory Impact Genetics, development, cancer Expand the scope of questions Challenges High-throughput User-assisted/automated tools Quantitative microCT Approach Processing pipeline Progression of tools Eviscerated Mouse Paw

2. IGP NCRR Capecchi Collaboration Activites Regular meetings with Capecchi lab staff and students Segmentations of wild-type and homozygous (Hox d11) mutant forelimbs Paper in progress: length comparison (digital) of mutant and wild-type forelimb bones

3. IGP NCRR Capecchi Collaboration Activities Shape analysis algorithms Paper accepted: Workshop on Mathematical Foundations of Computational Anatomy 2006 Shape analysis library Particle system and shape analysis code for ITK (supported locally by CIBC and NAMIC) Integrate into CIBC Segmentation application Shape analysis of hoxd11 vs. wild-type mouse forelimb bones

4. IGP NCRR Capecchi Collaboration Activities Image-basedOriginal Meta0.69 0.63 P10.780.79 P20.790.63 P31.080.99 Preliminary results for digit 2 from the image-based bone-length comparison study. Expressed as the ratio of the average length of the mutant bone to the average length of the wild type bone. Normalized with respect to the length of an individual specimen's humerus.

5. IGP NCRR Capecchi Collaboration Activities Homozygous Mutant Wild Type Segmentations of wild type and homozygous mutant bones of the second digit of the left mouse forelimb.

6. IGP NCRR Capecchi Collaboration Activities Length measurement of the mouse humerus using SCIRun.

7. IGP NCRR Capecchi Collaboration Plans A shape analysis pipeline for mouse phenotyping Open-source particle system library (Winter 2006) Further shape analysis research (ongoing) Specific scientific results for skeletal phenotyping with the Capecchi Laboratory Finish forelimb segmentations (Fall 2006) Bone length comparison paper (Fall 2006) Shape study of mouse bones (ongoing)

8. IGP NCRR Related Project: Bridge Reconstruction and visualization of microtubules from FLM Original Deconvolved Visualization of Block Traversal of Tubuli

9. IGP NCRR Related Projects: Chien,Marc Tracking of axons in SBFEMDeformable tiling and registration of serial section TEM Papers to appear in MICCAI 2006 Workshop on Microscopic Image Analysis