1. Anatomy & Neurobiology ANA 812 Lab Session 11 Peripheral Blood Bryan Guillory Lashaundra Thedford Philip Overall John Watson

2. Anatomy & Neurobiology Slide #42: Human Blood (Wright-Giemsa) Normal Blood (100X)

3. Anatomy & Neurobiology Slide #42: Human Blood (Wright-Giemsa) Platelets (Thrombocytes) (1000x) Platelets ● Small (2-4 μm) ● Granular

4. Anatomy & Neurobiology Slide #42: Human Blood (Wright-Giemsa) Erythrocytes (RBC’s) Normal Blood (400X) Erythrocytes -Biconcave Discs -Central Pallor

5. Anatomy & Neurobiology Slide #42: Human Blood (Wright-Giemsa) Normal Blood Erythrocytes (1000X) Biconcave Discs Central Pallor 7-8 um in diameter

6. Anatomy & Neurobiology Slide #43: Abnormal Human Blood (Wright-Giemsa) Anemic Blood (100X) Smaller RBC’s Larger Central Pallors Variable Size and Shape

7. Anatomy & Neurobiology Slide #43: Abnormal Human Blood (Wright-Giemsa) Anemic Blood (400X) Smaller RBC’s Larger Central Pallors Variable Size and Shape

8. Anatomy & Neurobiology Slide #43: Abnormal Human Blood (Wright-Giemsa) Anemic Blood (1000X) Various Sized RBC’s Larger Central Pallor Variable Size and Shape

9. Anatomy & Neurobiology Slide #42: Human Blood (Wright-Giemsa) Granulocytes: Neutrophils (400X) Neutrophils Granular Appearance

10. Anatomy & Neurobiology Slide #42: Human Blood (Wright-Giemsa) Banded Neutrophils (400X) “Stab Forms” Nucleus Elongated Less Prominent than Segmented Forms

11. Anatomy & Neurobiology Slide #43: Abnormal Human Blood (Wright-Giemsa) Anemic Blood (1000X) Segmented Neutrophil PMNs Lobed Nucleus w/a Fine Filament Between Most common Neutrophil

12. Anatomy & Neurobiology Slide #42: Human Blood (Wright-Giemsa) Agranulocytes: Lymphocytes (1000x) Lymphocyte ● Round Nucleus ● Cytoplasm (blue halo)

13. Anatomy & Neurobiology Slide #42: Human Blood (Wright-Giemsa) Agranulocytes: Monocytes (1000x) Monocyte ● Horseshoe, various shaped nucleus ● Large (14-20 μm)

14. Anatomy & Neurobiology Slide #42: Human Blood (Wright-Giemsa) Granulocytes: Eosinophil (1000x) Eosinophil Bi-lobed nucleus Granules

15. Anatomy & Neurobiology Electron Micrograph Granulocytes: Eosinophil Lysosomal crystals Granule Nucleus

16. Anatomy & Neurobiology Slide #42: Human Blood (Wright-Giemsa) Granulocytes: Basophil (1000x) Basophil ● “baso” because of blue-black granules when stained ● large granules that overlie nucleus (histamine) ● nucleus has smudged, indistinct chromatin pattern

17. Anatomy & Neurobiology Slide #41: Peripheral Blood Film – Human (Wright- Giemsa) 400x Monocyte