1. MRS Entrepreneurship Challenge: Lab-on-chip Sensor Technology Presented on December 6, 2005

2. Team Background Rachel Weaver, BME, MT Rachel Weaver, BME, MT Ryan Dempsey, BME, MT Ryan Dempsey, BME, MT Charles Bloom, BME, MT Charles Bloom, BME, MT John Richardson, BME, Economics John Richardson, BME, Economics Experienced in BioMEMs Experienced in BioMEMs Peter Shanahan, BME, Mathematics Peter Shanahan, BME, Mathematics Experienced in BioMEMs Experienced in BioMEMs

3. Entrepreneurship Challenge Scientists and business students will form “virtual teams” to develop a 12-slide PowerPoint presentation Scientists and business students will form “virtual teams” to develop a 12-slide PowerPoint presentation Present startup technology to a panel of venture capitalist judges at 2006 MRS spring meeting Present startup technology to a panel of venture capitalist judges at 2006 MRS spring meeting The grand prize is $3,000 The grand prize is $3,000 See http://www.mrs.org/entrepreneur/index.html for more information See http://www.mrs.org/entrepreneur/index.html for more informationhttp://www.mrs.org/entrepreneur/index.html

4. BioMEMs Technology BioMEMs: Biological micro-electromechanical systems BioMEMs: Biological micro-electromechanical systems Uses photolithography and soft-lithography Uses photolithography and soft-lithography Miniaturized device potential Miniaturized device potential Single cell reactions Single cell reactions Multiple experiments per chip Multiple experiments per chip In vitro apparatus simulates in vivo cellular environment In vitro apparatus simulates in vivo cellular environment

5. Clark Oxygen Sensor anode & cathode submersed in an electrolytic fluid anode & cathode submersed in an electrolytic fluid Linear relationship between [O 2 ] and current produced Linear relationship between [O 2 ] and current produced

6. Market potential Biological applications Biological applications Cell cultures Cell cultures Chemical applications Chemical applications Chemical reaction cost Chemical reaction cost Pharmaceutical uses Pharmaceutical uses Drug efficacy Drug efficacy

7. Project Cost 2” by 2” mask will cost roughly $600 2” by 2” mask will cost roughly $600 We will only use a fraction of the mask We will only use a fraction of the mask Each design iteration will run between $50 and $100 Each design iteration will run between $50 and $100 ~4-6 design iterations depending on time ~4-6 design iterations depending on time Includes 35 mm film costs Includes 35 mm film costs <$100 on office supplies, paper, etc. <$100 on office supplies, paper, etc.

8. Timeline

9. IWB Problem Formulation and Brainstorming Chart

10. References Fabrication of miniature Clark oxygen sensor integrated with microstructure Fabrication of miniature Clark oxygen sensor integrated with microstructure Ching-Chou Wu, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue Ching-Chou Wu, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue http://www.bme.vanderbilt.edu/srdesign/2005/group25/272_sdarticle.pdf http://www.bme.vanderbilt.edu/srdesign/2005/group25/272_sdarticle.pdf http://www.bme.vanderbilt.edu/srdesign/2005/group25/272_sdarticle.pdf A BioMEMS Review: MEMS Technology for Physiologically Integrated Devices A BioMEMS Review: MEMS Technology for Physiologically Integrated Devices AMY C. RICHARDS GRAYSON, REBECCA S. SHAWGO, AUDREY M. JOHNSON, NOLAN T. FLYNN, YAWEN LI, MICHAEL J. CIMA, AND ROBERT LANGER AMY C. RICHARDS GRAYSON, REBECCA S. SHAWGO, AUDREY M. JOHNSON, NOLAN T. FLYNN, YAWEN LI, MICHAEL J. CIMA, AND ROBERT LANGER http://www.bme.vanderbilt.edu/srdesign/2005/group25/BioMEMS_review.pdf http://www.bme.vanderbilt.edu/srdesign/2005/group25/BioMEMS_review.pdf http://www.bme.vanderbilt.edu/srdesign/2005/group25/BioMEMS_review.pdf