1. Let the evidence speak for itself.
Trace Evidence Basics
Let the evidence speak for itself.

2. Locard’s Exchange Principle
The value of trace (or contact) forensic evidence was first recognized by Edmund Locard in 1910.
The Locard’s Exchange Principle states that "with contact between two items, there will be an exchange.”
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3. Evidence
Think about what we have discussed and seen so far. List as many types of evidence you can.
Is the evidence class, individual or both?

4. Trace Evidence
Small but measurable amounts of physical or biological material found at a crime scene

5. Microscopic Examination
A compound microscope uses light reflected from the surface of a fiber and magnified through a series of lenses,
The comparison microscope (two compound microscopes joined by an optical bridge) is used for more precise identification. 
Electron microscopes either pass beams through samples to provide a highly magnified image, or reflect electrons off the sample's surface. 
A scanning electron microscope converts the emitted electrons into a photographic image for display.  This affords high resolution and depth of focus.

6. Spectrometer
The spectrometer, which separates light into component wavelengths. 
every organic element has a uniqueness to its constituent parts. 
By passing light through something to produce a spectrum, the analyst can read the resulting lines, called "absorption lines." 
That is, the specific wavelengths are characteristic of its component molecules of the substance. 

7. Micro-spectrophotometer
This microscope locates minute traces or shows how light interacts with the material under analysis. 
Linking this to a computerized spectrophotometer increases the accuracy.
  The scientist can get both a magnified visual and an infrared pattern at the same time, which increases the number of identifying characteristics of any given material.

8. Microscopic Image of Hairs & Fibers
Hairs and fibers may be transferred from the suspect or victim.
Hairs can be examined to identify their origin, such as human or animal. Hairs with roots intact can be tested for DNA.
Fibers may be natural fibers from plants or animals or synthetic fibers that are man-made.
Microscopic Image of Hairs & Fibers

9. Physical Match of Paint Chip Edges
Paint Evidence
Physical and chemical analysis of paint evidence (chips or residue) can indicate
Class characteristics : such as automobile paint, house paint, nail polish, etc. The evidence can be compared to 40,000 different types of paint classified in a database, which can be used to identify a particular make or model of car or brand of tool.
Individual characteristics : similarities between two samples, such as the color, number of layers, chemical composition, or a physical match between the edges of two paint chips – one from a tool and one from a crime scene.
Paint Transfer on a Car
Paint Layers
Physical Match of Paint Chip Edges
Did you know? Most paint evidence submitted to a lab will come from hit-and-run cases involving automobiles.  
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10. Magnified image of glass fragments
Glass Evidence
• Glass at a crime scene is analyzed to determine its color, surface characteristics, tint, thickness, density, chemical composition, and refractive index (RI).
The pattern of cracks in a windshield fracture can reveal information about speed, occupant position, and angle of impact.
The results of the tests provide clues about the crime and help investigators connect the evidence to a suspect or other object used in a crime, such as matching glass from a crime scene to a headlight to a suspect’s car.
Magnified image of glass fragments
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11. Impression Evidence
Shoeprints & Tire Tracks
• Impression evidence can be photographed, lifted with tape, or cast with plaster to compare to a suspect’s shoes or tires.
Investigators will examine the evidence to identify the brand of shoe or tire based on its tread pattern (class)
Wear patterns after being used for a period of time as well as other features (scratches, nicks, and cuts) can be used to suggest similarities between specific items (close to individual)

12. Microscopic Image of Sand
Dust & Dirt
Dust, dirt, or sand evidence can reveal where a person has traveled and may be picked up at a crime scene or left behind.
Investigators examine the samples for chemical composition, pollen, plant material, and other organic matter to find links to a specific crime scene.
Microscopic Image of Sand
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13. Other types of Evidence found at a crime scene

14. Fingerprints Tool Marks Bite Marks Wounds Insects Questioned Documents
Questioned Documents
DNA

15. Body Fluids
Ballistics
Explosives
Fracture Matches
Skeletal Remains