2. THYROID PHYSIOLOGY AND DEVELOPMENT Dr.m.ghasemi Ped endocrinologist Kermanshah university of medical science Dr.m.ghasemi Ped endocrinologist Kermanshah university of medical science

3. TRH, a tripeptide synthesized in the hypothalamus, stimulates the release of pituitary (TSH). Pituitary TSH is a glycoprotein that stimulates the synthesis and release of thyroid hormones by the thyroid gland. Thyroid function develops in three stages: Embryogenesis begins on the floor of the primitive oral cavity. The gland descends to its definitive position in the anterior lower neck by the end of the first trimester. Thyroid glands that do not reach the normal location are ectopic but may retain function; however, the glands most often become insufficient by early to mid childhood to support full thyroid secretion (a lingual or sublingual site or even tissue found in a thyroglossal duct cyst may be the only functioning thyroid gland). TRH, a tripeptide synthesized in the hypothalamus, stimulates the release of pituitary (TSH). Pituitary TSH is a glycoprotein that stimulates the synthesis and release of thyroid hormones by the thyroid gland. Thyroid function develops in three stages: Embryogenesis begins on the floor of the primitive oral cavity. The gland descends to its definitive position in the anterior lower neck by the end of the first trimester. Thyroid glands that do not reach the normal location are ectopic but may retain function; however, the glands most often become insufficient by early to mid childhood to support full thyroid secretion (a lingual or sublingual site or even tissue found in a thyroglossal duct cyst may be the only functioning thyroid gland).

4. The hypothalamic-pituitary-thyroid axis becomes functional in the second trimester. Peripheral metabolism of thyroid hormones matures in the third trimester. T 4, T 3, and TSH do not cross the placenta in significant amounts. Concentrations in fetal serum reflect primarily fetal secretion and metabolism. The hypothalamic-pituitary-thyroid axis becomes functional in the second trimester. Peripheral metabolism of thyroid hormones matures in the third trimester. T 4, T 3, and TSH do not cross the placenta in significant amounts. Concentrations in fetal serum reflect primarily fetal secretion and metabolism.

5. Maternal thyroid antibodies, iodides (including radioactive iodides), and medications given to mothers to treat hyperthyroidism (e.g., propylthiouracil, methimazole) cross the placenta and affect fetal thyroid function. An infant born prematurely or with IUGR may have an interruption of the normal maturational process and appear to have hypothyroidism by standard tests. Maternal thyroid antibodies, iodides (including radioactive iodides), and medications given to mothers to treat hyperthyroidism (e.g., propylthiouracil, methimazole) cross the placenta and affect fetal thyroid function. An infant born prematurely or with IUGR may have an interruption of the normal maturational process and appear to have hypothyroidism by standard tests.

6. The thyroid gland (1) concentrates iodine and (2) binds it (organifies it) to tyrosine molecules to produce either monoiodotyrosine or diiodotyrosine, with subsequent (3) coupling of two tyrosines, T 4 or T 3. The major fraction of circulating T 3 (approximately two thirds) is derived from peripheral deiodination of T 4 to T 3, but some is produced by the thyroid gland itself. In Graves disease, a larger fraction originates in the thyroid gland. The thyroid gland (1) concentrates iodine and (2) binds it (organifies it) to tyrosine molecules to produce either monoiodotyrosine or diiodotyrosine, with subsequent (3) coupling of two tyrosines, T 4 or T 3. The major fraction of circulating T 3 (approximately two thirds) is derived from peripheral deiodination of T 4 to T 3, but some is produced by the thyroid gland itself. In Graves disease, a larger fraction originates in the thyroid gland.

8. The conversion of T 4 to T 3 requires the removal of one iodine from the outer ring of tyrosine; removing an iodine from the inner ring results in reverse T 3, which has little biologic effect. Preferential conversion of T 4 to reverse T 3 rather than T 3 occurs in utero and in all forms of severe illness, including respiratory distress syndrome, fevers, anorexia, cachexia, and starvation. The conversion of T 4 to T 3 requires the removal of one iodine from the outer ring of tyrosine; removing an iodine from the inner ring results in reverse T 3, which has little biologic effect. Preferential conversion of T 4 to reverse T 3 rather than T 3 occurs in utero and in all forms of severe illness, including respiratory distress syndrome, fevers, anorexia, cachexia, and starvation.

9. Conversion from T 4 to T 3 increases immediately after birth and throughout life. T 4 and T 3 are noncovalently bound to a specific serum carrier protein, T 4 -binding globulin, and, to a lesser extent, albumin. Only small (<0.02%) fractions of T 4 and T 3 are not bound; free T 4 and free T 3 are biologically active. Free T 3 exerts metabolic effects and negative feedback on TSH release Conversion from T 4 to T 3 increases immediately after birth and throughout life. T 4 and T 3 are noncovalently bound to a specific serum carrier protein, T 4 -binding globulin, and, to a lesser extent, albumin. Only small (<0.02%) fractions of T 4 and T 3 are not bound; free T 4 and free T 3 are biologically active. Free T 3 exerts metabolic effects and negative feedback on TSH release

10. Serum TSH increases just after birth, but soon decreases to lower values considered normal for later life. T 4 secretion increases after birth, partially as a result of the peak in TSH and partially because of maturation of thyroid metabolism. Serum thyroid hormone concentrations decrease, but only slowly reach values routinely found in adults. Free T 4 is the test of choice because it eliminates the effects of variation in protein binding, which can be substantial. Serum TSH increases just after birth, but soon decreases to lower values considered normal for later life. T 4 secretion increases after birth, partially as a result of the peak in TSH and partially because of maturation of thyroid metabolism. Serum thyroid hormone concentrations decrease, but only slowly reach values routinely found in adults. Free T 4 is the test of choice because it eliminates the effects of variation in protein binding, which can be substantial.

11. plasma concentrations of TSH above the normal range indicate primary hypothyroidism, and concentrations below the normal range most often indicate the presence of hyperthyroidism. Although a thyroid scan rarely is indicated in the evaluation of pediatric thyroid disease, thyroid agenesis, ectopic thyroid tissue and the diagnoses of hyperfunctioning "hot" nodules or of nonfunctioning "cold" nodules may be detected by this test. A thyroid scan performed with the short-lived isotope radioactive iodine ( 123 I) indicates the size, shape, and location of the thyroid gland and iodine concentrating ability. plasma concentrations of TSH above the normal range indicate primary hypothyroidism, and concentrations below the normal range most often indicate the presence of hyperthyroidism. Although a thyroid scan rarely is indicated in the evaluation of pediatric thyroid disease, thyroid agenesis, ectopic thyroid tissue and the diagnoses of hyperfunctioning "hot" nodules or of nonfunctioning "cold" nodules may be detected by this test. A thyroid scan performed with the short-lived isotope radioactive iodine ( 123 I) indicates the size, shape, and location of the thyroid gland and iodine concentrating ability.

12. A solitary nodule is a source of concern for the possibility of cancer, especially if it is solid and nonfunctional. Ultrasound may determine whether it is cystic or solid. If the nodule is solid, a 123 I scan indicates its functional status. Excisional biopsies usually are performed on solitary nodules. Scans are rarely indicated in the diagnosis of Hashimoto thyroiditis or thyrotoxicosis. A solitary nodule is a source of concern for the possibility of cancer, especially if it is solid and nonfunctional. Ultrasound may determine whether it is cystic or solid. If the nodule is solid, a 123 I scan indicates its functional status. Excisional biopsies usually are performed on solitary nodules. Scans are rarely indicated in the diagnosis of Hashimoto thyroiditis or thyrotoxicosis.

14. Hypothyroidism Hypothyroidism is diagnosed by a decreased serum free T 4 and may be the result of: diseases of the thyroid gland (primary hypothyroidism), abnormalities of the pituitary gland (secondary) or abnormalities of the hypothalamus (tertiary). Hypothyroidism is congenital or acquired and may be associated with a goiter Hypothyroidism is diagnosed by a decreased serum free T 4 and may be the result of: diseases of the thyroid gland (primary hypothyroidism), abnormalities of the pituitary gland (secondary) or abnormalities of the hypothalamus (tertiary). Hypothyroidism is congenital or acquired and may be associated with a goiter

15. Congenital Hypothyroidism Congenital hypothyroidism occurs in approximately 1 in 4000 live births and is caused by dysgenesis, disorders of embryogenesis (agenesis, aplasia, ectopia), far more often than by dyshormonogenesis disorders (e.g., enzyme defects). Thyroid tissue usually is not palpable in these sporadic nongoitrous conditions. Dyshormonogenesis, disorders of intrathyroid metabolism or goitrous congenital hypothyroidism, occurs in about 1 in 30,000 live births. Congenital hypothyroidism occurs in approximately 1 in 4000 live births and is caused by dysgenesis, disorders of embryogenesis (agenesis, aplasia, ectopia), far more often than by dyshormonogenesis disorders (e.g., enzyme defects). Thyroid tissue usually is not palpable in these sporadic nongoitrous conditions. Dyshormonogenesis, disorders of intrathyroid metabolism or goitrous congenital hypothyroidism, occurs in about 1 in 30,000 live births.

16. The goiter reflects an inborn error of metabolism in the pathway of iodide incorporation or thyroid hormone biosynthesis or reflects the transplacental passage of antithyroid drugs given to the mother. The free T 4 concentration is low, and the TSH level is elevated. Routine neonatal screening programs to measure cord blood or heel-stick TSH values occur in every state in the United States. An immediate confirmatory serum sample should be obtained from any infant having a positive result on a screening test. A low T 4 and high TSH confirm the finding. The goiter reflects an inborn error of metabolism in the pathway of iodide incorporation or thyroid hormone biosynthesis or reflects the transplacental passage of antithyroid drugs given to the mother. The free T 4 concentration is low, and the TSH level is elevated. Routine neonatal screening programs to measure cord blood or heel-stick TSH values occur in every state in the United States. An immediate confirmatory serum sample should be obtained from any infant having a positive result on a screening test. A low T 4 and high TSH confirm the finding.

19. Isolated secondary or tertiary hypothyroidism is rare, occurring in 1 in 100,000 live births; the free T 4 is normal to low. When tertiary or secondary hypothyroidism is detected, assessment of other pituitary hormones and investigation of pituitary- hypothalamic anatomy via MRI are indicated. is rare, occurring in 1 in 100,000 live births; the free T 4 is normal to low. When tertiary or secondary hypothyroidism is detected, assessment of other pituitary hormones and investigation of pituitary- hypothalamic anatomy via MRI are indicated.

20. congenital T 4 -binding globulin deficiency about 1 in 10,000 live births and is associated with a low serum total T 4 concentration, a normal TSH and serum free T 4, and a euthyroid status. This entity does not require treatment with thyroid hormone because it is merely a binding protein abnormality. It is commonly X-linked dominant. about 1 in 10,000 live births and is associated with a low serum total T 4 concentration, a normal TSH and serum free T 4, and a euthyroid status. This entity does not require treatment with thyroid hormone because it is merely a binding protein abnormality. It is commonly X-linked dominant.

21. Clinical manifestations of congenital hypothyroidism in the immediate newborn period usually are subtle but become more evident weeks or months after birth. Newborn screening is crucial to make an early diagnosis and initiate thyroid replacement therapy by younger than 1 month of age. Clinical manifestations of congenital hypothyroidism in the immediate newborn period usually are subtle but become more evident weeks or months after birth. Newborn screening is crucial to make an early diagnosis and initiate thyroid replacement therapy by younger than 1 month of age.

22. Findings at various stages after birth include : gestation greater than 42 weeks, birth weight greater than 4 kg, hypothermia, is, respiratory distress, large posterior fontanelle, acrocyanos abdominal distention, lethargy and poor feeding, jaundice more than 3 days after birth, edema, umbilical hernia, mottled skin, constipation, large tongue, dry skin, and hoarse cry. Thyroid hormones are crucial for maturation and differentiation of tissues such as: bone (the bone age is often delayed at birth because of intrauterine hypothyroidism) and brain (most thyroid-dependent brain maturation occurs 2 to 3 years after birth) Findings at various stages after birth include : gestation greater than 42 weeks, birth weight greater than 4 kg, hypothermia, is, respiratory distress, large posterior fontanelle, acrocyanos abdominal distention, lethargy and poor feeding, jaundice more than 3 days after birth, edema, umbilical hernia, mottled skin, constipation, large tongue, dry skin, and hoarse cry. Thyroid hormones are crucial for maturation and differentiation of tissues such as: bone (the bone age is often delayed at birth because of intrauterine hypothyroidism) and brain (most thyroid-dependent brain maturation occurs 2 to 3 years after birth)

25. When treatment is initiated within 1 month or less after birth, the prognosis for normal intellectual development is excellent; screening programs usually offer therapy within 1 to 2 weeks of birth. If therapy is instituted after 6 months, when the signs of severe hypothyroidism are present, the likelihood of normal intellectual function is markedly decreased. Growth improves after thyroid replacement, even in late diagnosed cases. When treatment is initiated within 1 month or less after birth, the prognosis for normal intellectual development is excellent; screening programs usually offer therapy within 1 to 2 weeks of birth. If therapy is instituted after 6 months, when the signs of severe hypothyroidism are present, the likelihood of normal intellectual function is markedly decreased. Growth improves after thyroid replacement, even in late diagnosed cases.

26. The dose of T 4 changes with age; 10 to 15 μg/kg of T 4 is used for a newborn, but about 3 μg/kg is used later in childhood. In neonatal hypothyroidism, the goal is to bring the serum free T 4 rapidly into the upper half of the range of normal. Suppression of TSH is not seen in all cases and is not necessary in all cases because such suppression may lead to excessive doses of T 4. The dose of T 4 changes with age; 10 to 15 μg/kg of T 4 is used for a newborn, but about 3 μg/kg is used later in childhood. In neonatal hypothyroidism, the goal is to bring the serum free T 4 rapidly into the upper half of the range of normal. Suppression of TSH is not seen in all cases and is not necessary in all cases because such suppression may lead to excessive doses of T 4.

27. Acquired Hypothyroidism The clinical manifestations may be subtle. Hypothyroidism should be suspected in any child who has a decline in growth velocity, especially if not associated with weight loss. The most common cause of acquired hypothyroidism in older children in the United States is lymphocytic autoimmune thyroiditis (Hashimoto thyroiditis). In many areas of the world, iodine deficiency is the etiology of endemic goiter (endemic cretinism). The failure of the thyroid gland may be heralded by an increase of TSH before T 4 levels decrease. In contrast to untreated congenital hypothyroidism, acquired hypothyroidism is not a cause of permanent developmental delay. The clinical manifestations may be subtle. Hypothyroidism should be suspected in any child who has a decline in growth velocity, especially if not associated with weight loss. The most common cause of acquired hypothyroidism in older children in the United States is lymphocytic autoimmune thyroiditis (Hashimoto thyroiditis). In many areas of the world, iodine deficiency is the etiology of endemic goiter (endemic cretinism). The failure of the thyroid gland may be heralded by an increase of TSH before T 4 levels decrease. In contrast to untreated congenital hypothyroidism, acquired hypothyroidism is not a cause of permanent developmental delay.

28. Hashimoto Thyroiditis Also known as autoimmune or lymphocytic thyroiditis, Hashimoto thyroiditis is a common cause of goiter and acquired thyroid disease in older children and adolescents. A family history of thyroid disease is present in 25% to 35% of patients. The etiology is an autoimmune process targeted against the thyroid gland with lymphocytic infiltration and lymphoid follicle and germinal center formation preceding fibrosis and atrophy. Also known as autoimmune or lymphocytic thyroiditis, Hashimoto thyroiditis is a common cause of goiter and acquired thyroid disease in older children and adolescents. A family history of thyroid disease is present in 25% to 35% of patients. The etiology is an autoimmune process targeted against the thyroid gland with lymphocytic infiltration and lymphoid follicle and germinal center formation preceding fibrosis and atrophy.

29. Clinical manifestations include a firm, nontender euthyroid, hypothyroid, or, rarely, hyperthyroid (hashitoxicosis) diffuse goiter with a pebble- like feeling; an insidious onset after 6 years of age (the incidence peaks in adolescence, with a female predominance); and sometimes a pea-sized delphian lymph node above the thyroid isthmus. Associated autoimmune diseases include diabetes mellitus type 1 (DM1), adrenal insufficiency (Schmidt syndrome), and hypoparathyroidism. include a firm, nontender euthyroid, hypothyroid, or, rarely, hyperthyroid (hashitoxicosis) diffuse goiter with a pebble- like feeling; an insidious onset after 6 years of age (the incidence peaks in adolescence, with a female predominance); and sometimes a pea-sized delphian lymph node above the thyroid isthmus. Associated autoimmune diseases include diabetes mellitus type 1 (DM1), adrenal insufficiency (Schmidt syndrome), and hypoparathyroidism.

30. polyglandular syndrome type I consists of hypoparathyroidism, Addison disease, mucocutaneous candidiasis, and often hypothyroidism. Autoimmune polyglandular syndrome type II consists of Addison disease, DM1, and frequently autoimmune hypothyroidism. Trisomy 21 and Turner syndrome predispose to the development of autoimmune thyroiditis. polyglandular syndrome type I consists of hypoparathyroidism, Addison disease, mucocutaneous candidiasis, and often hypothyroidism. Autoimmune polyglandular syndrome type II consists of Addison disease, DM1, and frequently autoimmune hypothyroidism. Trisomy 21 and Turner syndrome predispose to the development of autoimmune thyroiditis.

31. The diagnosis may be confirmed by serum antiTPO(previously antimicrosomal) and antiTG antibodies. Neither biopsy nor thyroid scan is indicated in Hashimoto thyroiditis, although the thyroid scan with reduced uptake may differentiate hashitoxicosis from Graves disease. The diagnosis may be confirmed by serum antiTPO(previously antimicrosomal) and antiTG antibodies. Neither biopsy nor thyroid scan is indicated in Hashimoto thyroiditis, although the thyroid scan with reduced uptake may differentiate hashitoxicosis from Graves disease.

32. Treatment with thyroid hormone sufficient to normalize TSH and free T 4 is indicated for hypothyroidism in Hashimoto thyroiditis. Patients without manifestation of hypothyroidism require periodic thyroid function testing (serum TSH and free T 4 ) every 6 to 12 months to detect the later development of hypothyroidism. Goiter with a normal TSH usually is not an indication for treatment Treatment with thyroid hormone sufficient to normalize TSH and free T 4 is indicated for hypothyroidism in Hashimoto thyroiditis. Patients without manifestation of hypothyroidism require periodic thyroid function testing (serum TSH and free T 4 ) every 6 to 12 months to detect the later development of hypothyroidism. Goiter with a normal TSH usually is not an indication for treatment