1. Establishing simian NK cell parameters for the study of NK-1R antagonists in vivo Jordan Orange, MD, PhD and Steven D. Douglas, MD November 16, 2005

2. NK Cells Cytotoxicity - Perforin-mediated Antibody dependent (ADCC) - Trail/FAS-L-mediated - TNF-mediated Cytokine Production (NK1/NK2) - IFN- , TNF, IL-5, IL-13 Costimulation - CD40L (CD154) - OX40L J.S. Orange, in Up to Date, Rose, B.D. (ed) 2004 v12.1 NK K562 NK cells are lymphocytes important in immune regulation and host defense that are capable of being specifically activated or inhibited after the ligation of germline-encoded receptors.

3. J.S. Orange, and Z.K. Ballas 2005 Clinical Immunology v117

4. K562 Lytic Units 20 Per PBMC NK cell cytotoxicity

5. Paradigms of NK cell impairment during retroviral infection Expression returns after prolonged aviremia

6. NCRs in Simian NK cell populations

7. Simian Human

8. Simian Human

9. RDL P815 Blocking FO1 melanoma IgG  CD16  NKp30  NKp46

10. BT25 - Uninfected Whole blood analysis

11. IgG1 NKp30 CD16 NKp46 NKp44 29.8 17 607 46 7 BT25 - Uninfected Whole blood analysis

12. BT25 - Uninfected Whole blood analysis

13. Controversies in defining a Simian NK cell

15. % C16+/CD3- NK cell percentages

16. % of C16+/CD3- positive for NKp46 NKp46

17. % of C16+/CD3- positive for NKp30 NKp30

18. NOTE: ALL OF THESE SAMPLES ARE EXTRACTED & UNDILUTED; All samples had floaters after resuspension with EIA buffer. IPCP SP RUN #20

19. NOTE: ALL OF THESE SAMPLES ARE EXTRACTED & UNDILUTED; All samples had floaters after resuspension with EIA buffer. IPCP SP RUN #20

20. IPCP SP RESULTS 1 Samples BV01 and CA84 were combined because we were not sent enough sample to assay individually

21. IPCP SP RESULTS 2 Samples CE48 and DD95 were combined because we were not sent enough sample to assay individually 3 Original run done on EDTA plasma; re-run done on HEP plasma

22. IPCP SP RESULTS 4 SP run results were invalid – need to be repeated * Bloods were shipped by Tulane on ice packs; they were cold upon arrival at CHOP

23. SP NK-1R 2 3 1 NK cell Activating receptor Cytotoxicity Cytokine production Activating receptor expression Inhibitory receptor expression Ca ++ flux Chemotaxis Aim 3a SP inhib. Signal-mediated SPA NK-1R signaling Activation signaling

24. NK cell SP NK-1R Cytotoxicity Activating receptor expression 2 1 On treatment Pre-treatment Post-infection in treatment evaluation Aim 3b HIV infected cell SPA