1. Dasty2 DAS workshop 2009 10 th March Rafael Jimenez

2. Dasty2 It is an Ajax web-based protein DAS client. Protein sequence positional features Protein sequence non-positional features Protein 3D structures Dasty2 Proxy Internet DAS Registry DAS Ontology PDB DAS Reference server DAS Alignment server Dasty2 client DAS Annot. servers DAS Annot. servers DAS Annot. servers DAS Annot. servers DAS Annot. servers DAS Annot. servers DAS Annot. servers DAS Annot. servers DAS Annot. servers

3. Implementation Service broker Service consumer Service provider Service Contract... Interact Publish Find Service Oriented Architecture

4. Registry ClientSources DAS... Publish Find Distributed Annotation System Implementation Interact DAS Registry Uniprot-PDB Alignments Dasty2 Protein Sequence Type DAS Protein Ontology PDB

5. Client Extensions PDB DAS PDB-Uniprot align. DAS Protein Ontology GO (Gene Ontology) OLS (Ontology Lookup Service) Filtering Query tool 3D view Search and configuration Annotation writeback Dasty2 DAS registry DAS Protein Ontology PICR (Protein Cross- Reference service) OpenID DAS writeback 3D protein view Feature filtering Visual ontology query tool (Ontodas) Advance search and configuration (myDasty) Annotation writeback myDasty Ontodas

6. 3D view

7. Filtering

8. Query tool

11. Search and configuration

12. Search and configuration

13. Annotation writeback Gustavo Salazar Presentation March 10th 14:10

14. Thank you!

15. Supervision - Henning Hermjakob, EMBL-EBI, UK - Alexander Garcia, Bremen University, Germany - Nicky Mulder, CBIO, UCT, South Africa - Edwin Blake, Computer Science Department, UCT, South Africa Acknowledgments Collaborations - Alberto Labarga, - Andreas Prlic, - Eugene Kulesha, - Nisha Vinod, - Philip Jones, - Andreas Kahari, -Andy Jenkinson Funding and collaborations Ministerio de Educacion y Ciencia BioSapiens Network of Excellence European Commission (FP6 Programme) Proteomics ServicesEBI-EMBL National Bioinformatics Network of South Africa Contributions - Antony Quinn, EMBL-EBI, UK - Fernando Martinez, NBN central node, South Africa - Gustavo A. Salazar, CBIO, UCT, South Africa - Kieran O'Neill, BCCRC, Canada - Omar Pera, Universidad de Valencia, Spain - Rafael C. Jimenez, CIPF, Valencia, Spain UCT South Africa CBIO, UCT South Africa CIPF, Spain