1. FlaxLic ® Equine Research

2. Dr. Gregg VeneklasenDr. Gregg Veneklasen Timber Creek Veterinary Hospital, Canyon, TXTimber Creek Veterinary Hospital, Canyon, TX Reproductive specialistReproductive specialist Embryo transfer business (horses & wildlife)Embryo transfer business (horses & wildlife) Has experienced favorable improvements in reproductive performance by feeding FlaxLic ®Has experienced favorable improvements in reproductive performance by feeding FlaxLic ®

3. Study Background OBJECTIVE: Measure changes in blood fatty acids in brood mares supplemented with FlaxLic ®OBJECTIVE: Measure changes in blood fatty acids in brood mares supplemented with FlaxLic ® 30 brood mares fed a common diet30 brood mares fed a common diet Two treatments:Two treatments: –15 Control –15 FlaxLic ® 21-day feeding study21-day feeding study

4. Study Background Blood sampledBlood sampled –days 0, 7, 14, and 21 –Obtained by TCVH staff –Plasma recovered via centrifugation –Frozen and shipped via overnight courier to KSU Plasma analyzed for fatty acid profiles by gas chromatographyPlasma analyzed for fatty acid profiles by gas chromatography

6. Total Fatty Acids No effect of treatment, P > 0.10.

7. Palmitic Acid No effect of treatment, P > 0.10.

8. Stearic Acid No effect of treatment, P > 0.10.

9. Linoleic Acid (omega-6) No effect of treatment, P > 0.10.

10. Arachidonic Acid No treatment effect, P > 0.10.

11. Alpha Linolenic Acid (omega-3) Effect of treatment, P < 0.01.

12. Eicosapentaenoic Acid No treatment effect, P > 0.10.

13. Docosahexaenoic Acid No treatment effect, P > 0.10.

14. Total Omega-3 Fatty Acids Effect of treatment, P < 0.01.

15. Total Omega-3 Fatty Acids Alpha Linolenic Acid Eicosapentaenoic Acid Docosapentaenoic Acid Relative Contributions to Omega-3 Fatty Acid Increase

16. FlaxLic ®