1. ControlDrug treated 2D gel images of bacterial protein sample Set of gel images for analysis

2. Steps followed in gel analysis 1) Set up Project 2) Detect and Quantify Spots 3) Analyze Expression Profiles 4) Present Results

3. ANALYSIS, RESULT AND DATA INTERPRETATION

4. Project Name Bacterial sample 2D-Gels for Analysis. Project Creation Date …………………………. Author …………………………. Description Bacterial sample Control vs Drug treated Use internal Standard No Pool C\Users\XYZ Groups Control (1 image) 780spots transferred from fused image 96 spots cancelled Drug Treated (1 image) 780 spots transferred from fused image 96 spots cancelled Vector Matching :245 1. Project Summary:

5. Profile Spot Information Control Totarol treated Spot ID : 123 pI : 5.26 MW : 65.1k Da Spot ID : 2082 pI : 5.28 MW : 44.8k Da 2. Spot details Control Totarol Fused 3D view

6. 4.004.254.504.755.005.255.505.756.006.256.506.757.00 80 75 70 65 60 55 50 45 40 35 30 25 20 15 10 5 pI index Molecular weight (kDal) 3.Figure showing Molecular weight to pI 85 pI : ~4.53 Mol. weight : 24.9 kDal pI : ~4.90 Mol. weight : 75.2 kDal

7. No. of spots showing up regulation Up regulation No. of folds (and above)No.of spots 2142 471 636 826 1016 12 1510 4.1. Differential expression : Up regulation Up regulation x Folds or above

8. 4.2. expression profile: Up regulation(8-10 folds up) -10 spots Mean normalized value of group-Control Mean normalized value of group 2 (Drug Treated) Ratio = ABCDE FGHIJ

9. No. of spots showing up regulation Down regulation No. of folds (and above)No.of spots 2174 447 620 812 108 124 151 4.3.Differential expression : Down regulation Down regulation x Folds or above

10. 4.4.Expression profile: Down regulation(5-6 folds down) -10 spots ABCDE FGHIJ Mean normalized value of group-Control Mean normalized value of group 2 (Drug Treated) Ratio =

11. Hierarchical Cluster Analysis HCL Tree 5.Cluster Analysis

12. 6. Scatter plot