1. DAVID Bioinformatics Web Site 2012 – 2015 David Huang, MD LMS/CCR/NCI Huang.Dawei@nih.gov

2. The HIV-1 envelope protein gp120 impairs B cell proliferation by inducing TGF-β1 production and FcRL4 expression. Nature Immunology 14, 1256–1265 (2013) HIV envelope induces a cascade of cell signals in non-proliferating target cells that favor virus replication. Proc Natl Acad Sci U S A. 9;99(14):9380-5 (2002) Exampled Studies: DAVID Manuals: Extracting biological meaning from large gene lists with DAVID Current Protocols in Bioinformatics, UNIT 13.11 (2009) Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources Nature Protocols 4, - 44 - 57 (2009)

3. Enrichment Analysis Red ball NOT enriched Red balls are enriched 10% 40%

4. Demonstration  Gene Centric Analysis  Annotation Centric Analysis  Pathway View