1. Supplementary Material 3 Gene ontology annotation of cellular component, molecular function and biological processes for both hypoxia and NAP supplemented hypoxia after 3, 6 and 12 h of hypobaric hypoxia exposure. All the identified pathways were sorted according to the significance value.

2. 3h hypoxia Cellular Component IDNameP-value Hit Count in Query List Hit Count in GenomeHit in Query List GO:0005739 mitochondrion 0161483 CRYM,AK1,GAPDH,SOD1,COX6A1,C OX5A,ATP5C1,NDUFA10,GPD1,COX5 B,PPP3CA,DPYSL2,MCL1,ALKBH3,MP ST,PSMA6 GO:0031966 mitochondrial membrane 0.0025277478 COX6A1,COX5A,ATP5C1,NDUFA10,C OX5B,MCL1,MPST GO:0005743 mitochondrial inner membrane 0.0046736352 COX6A1,COX5A,ATP5C1,NDUFA10,C OX5B,MPST GO:0019773 proteasome core complex, alpha-subunit complex 0.01212628 PSMA6,PSMA1 GO:0005833 hemoglobin complex 0.033592213 HBB,HBA1 3h hypoxia + NAP Cellular Component IDNameP-value Hit Count in Query List Hit Count in GenomeHit in Query List GO:0005739 mitochondrion 0.000047151483 CRYM,AK1,PSMC2,GAPDH,SOD1,PRD X2,COX5A,COX6A1,ATP5C1,NDUFA1 0,GPD1,COX5B,PPP3CA,DPYSL2,MPS T GO:0005625 soluble fraction 0.0014068480 ENO1,PAFAH1B1,GPD1,PPP3CA,DPYS L2,HBA1,TPI1,ACTB GO:0043005 neuron projection 0.00578583 AK1,PTK2B,SYT1,CA2,SOD1,PAFAH1 B1,DPYSL2,ACTB GO:0042995 cell projection 0.013893101069 AK1,PTK2B,SYT1,CA2,SOD1,CDC42,P AFAH1B1,GNB1,DPYSL2,ACTB GO:0005743 mitochondrial inner membrane 0.0188336352 COX5A,COX6A1,ATP5C1,NDUFA10,C OX5B,MPST GO:0000267 cell fraction 0.040058111462 VCP,ENO1,GDI1,PAFAH1B1,GPD1,PPP 3CA,DPYSL2,GNAO1,HBA1,TPI1,ACT B GO:0019717 synaptosome 0.04274141 ENO1,GDI1,DPYSL2,GNAO1 Table 1. Gene ontology annotation of the differentially expressed proteins of 3h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their cellular component.

3. Figure 1. Gene ontology annotation of the differentially expressed proteins of 3h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their cellular component (3H hypoxia and 3HT-NAP supplemented hypoxia).

4. 3h hypoxia Molecular Function IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO:0016491Oxidoreductase activity0.0011719728 GAPDH,SOD1,COX6A1,COX5A,NDUF A10,GPD1,COX5B,AKR1B1,ALKBH3 GO:0004129Cytochrome-c oxidase activity0.004343329COX6A1,COX5A,COX5B GO:0016676 Oxidoreductase activity, acting on a heme group of donors, oxygen as acceptor 0.004343329COX6A1,COX5A,COX5B GO:0015078 Hydrogen ion transmembrane transporter activity 0.0113784112COX6A1,COX5A,ATP5C1,COX5B 3h hypoxia +NAP Molecular Function IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO:0004129Cytochrome-c oxidase activity0.00913329COX5A,COX6A1,COX5B GO:0016675 Oxidoreductase activity, acting on a heme group of donors 0.010128330COX5A,COX6A1,COX5B GO:0042802Identical protein binding0.0225149833 VCP,CRYM,SYT1,ENO1,SOD1,PAFAH 1B1,GPD1,KITLG,DNM1 GO:0015078 Hydrogen ion transmembrane transporter activity 0.0290564112COX5A,COX6A1,ATP5C1,COX5B Table 2. Gene ontology annotation of the differentially expressed proteins of 3h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their molecular function.

5. Figure 2. Gene ontology annotation of the differentially expressed proteins of 3h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their molecular function (3H hypoxia and 3HT-NAP supplemented hypoxia).

6. 3h hypoxia Biological Process IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO:0006091 Generation of precursor metabolites and energy 0.0131037443 GAPDH,COX6A1,COX5A,ATP5C1,ND UFA10,GNB1,COX5B GO:0055114Oxidation-reduction process0.00004912934 GAPDH,SOD1,COX6A1,COX5A,ATP5 C1,NDUFA10,GPD1,GNB1,COX5B,AK R1B1,ALKBH3,CAB39 GO:0022904 Respiratory electron transport chain 0.0018675113 COX6A1,COX5A,ATP5C1,NDUFA10, COX5B GO:0042542Response to hydrogen peroxide0.012267478HBB,PTK2B,SOD1,GNAO1 GO:0000302 Response to reactive oxygen species 0.0443314108HBB,PTK2B,SOD1,GNAO1 GO:0015980 Energy derivation by oxidation of organic compounds 0.0218786318 COX6A1,COX5A,ATP5C1,NDUFA10, GNB1,COX5B GO:0051592Response to calcium ion0.008916472PTK2B,SYT1,GDI1,ACTB 3h hypoxia +NAP Biological Process IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO:0006091 Generation of precursor metabolites and energy 0.0006369443 GAPDH,ENO1,COX5A,COX6A1,ATP5 C1,NDUFA10,GNB1,COX5B,TPI1 GO:0055114Oxidation-reduction process0.00083112934 GAPDH,SOD1,PRDX2,COX5A,COX6 A1,ATP5C1,NDUFA10,GPD1,GNB1,C OX5B,TPI1,CAB39 GO:0022904 Respiratory electron transport chain 0.0067155113 COX5A,COX6A1,ATP5C1,NDUFA10, COX5B GO:0042542Response to hydrogen peroxide0.00107578HBB,PTK2B,SOD1,PRDX2,GNAO1 GO:0000302 Response to reactive oxygen species 0.0053775108HBB,PTK2B,SOD1,PRDX2,GNAO1 GO:0051592Response to calcium ion0.025706472PTK2B,SYT1,GDI1,ACTB GO:0009117Nucleotide metabolic process0.00071612921 VCP,AK1,PSMC2,PTK2B,GDI1,ATP5C 1,PAFAH1B1,GPD1,GNAO1,TPI1,CMP K1,DNM1 GO:0019752 Carboxylic acid metabolic process 0.02083310862 PSMC5,CRYM,PSMC2,GAPDH,ENO1, SOD1,GPD1,GSTP1,TPI1,CAB39 GO:0044093 Positive regulation of molecular function 0.0168210841 PSMC5,VCP,PSMC2,PTK2B,ENO1,SO D1,PRDX2,GNB1,GNAO1,KITLG Table 3. Gene ontology annotation of the differentially expressed proteins of 3h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their biological process.

7. Figure 3. Gene ontology annotation of the differentially expressed proteins of 3h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their biological process (3H hypoxia and 3HT-NAP supplemented hypoxia).

8. 6h hypoxia Cellular Component IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO:0005739Mitochondrion0191483 AK1,PSMC2,GAPDH,SOD1,COX5A,COX6A1,ATP 5C1,PRDX5,NDUFA10,DDAH1,GPD1,COX5B,DP YSL2,MCL1,UQCRFS1,PEBP1,ALKBH3,MPST,GL UL GO:0031966Mitochondrial membrane0.0000279478 COX5A,COX6A1,ATP5C1,NDUFA10,COX5B,MCL 1,UQCRFS1,PEBP1,MPST GO:0030424Axon0.0016016278PTK2B,CA2,DPYSL2,PEBP1,GLUL,ACTB GO:0005625Soluble fraction0.0036317480GPD1,RAB1A,DPYSL2,PEBP1,HBA1,GLUL,ACTB GO:0019717Synaptosome0.0172854141GDI1,DPYSL2,PEBP1,GLUL GO:0000502Proteasome complex0.031211366VCP,PSMC2,PSMA1 GO:0005746Mitochondrial respiratory chain0.038723371COX6A1,NDUFA10,UQCRFS1 GO:0043005Neuron projection0.0014228583 AK1,PTK2B,CA2,SOD1,DPYSL2,PEBP1,GLUL,AC TB GO:0044429Mitochondrial part0.00007510710 SOD1,COX5A,COX6A1,ATP5C1,NDUFA10,COX5 B,MCL1,UQCRFS1,PEBP1,MPST GO:0031967Organelle envelope0.0013449769 COX5A,COX6A1,ATP5C1,NDUFA10,COX5B,MCL 1,UQCRFS1,PEBP1,MPST GO:0042995Cell projection0.0175391069 AK1,PTK2B,CA2,SOD1,CDC42,DPYSL2,PEBP1,G LUL,ACTB GO:0005833Hemoglobin complex0.031075213HBB,HBA1 6h hypoxia + NAP Cellular Component IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO:0005739Mitochondrion0251483 PSMC2,GAPDH,RHOA,COX5A,ACADL,UQCRFS1,PEBP1,ALKBH3,MPST,CRYM,AK1,NME1,SOD1, ACOT7,PRDX2,COX6A1,ATP5C1,DDAH1,COX5B,PPP3CA,DPYSL2,MCL1,PSMA6,GLUL,MDH1 GO:0005625Soluble fraction0.00002211480 ENO1,RHOA,PAFAH1B1,PEBP1,ACTB,NME1,PPP 3CA,DPYSL2,HBA1,GLUL,MDH1 GO:0000502Proteasome complex0.000226566PSMC5,VCP,PSMC2,PSMA6,PSMA1 GO:0019717Synaptosome0.000576141ENO1,PEBP1,GDI1,DPYSL2,GNAO1,GLUL GO:0031966Mitochondrial membrane0.0016379478 COX5A,ACADL,UQCRFS1,PEBP1,MPST,COX6A1,ATP5C1,COX5B,MCL1 GO:0044429Mitochondrial part0.00622710710 COX5A,ACADL,UQCRFS1,PEBP1,MPST,SOD1,C OX6A1,ATP5C1,COX5B,MCL1 GO:0000267Cell fraction0.011178141462 VCP,ENO1,RHOA,PAFAH1B1,PEBP1,ACTB,NME 1,GDI1,PPP3CA,DPYSL2,GNAO1,HBA1,GLUL,M DH1 GO:0031967Organelle envelope0.01214510769 COX5A,ACADL,PAFAH1B1,UQCRFS1,PEBP1,MP ST,COX6A1,ATP5C1,COX5B,MCL1 GO:0030424Axon0.02586278RHOA,PAFAH1B1,PEBP1,ACTB,DPYSL2,GLUL GO:0005624Membrane fraction0.04016111067 VCP,ENO1,RHOA,PAFAH1B1,PEBP1,NME1,GDI1,PPP3CA,DPYSL2,GNAO1,GLUL GO:0043005Neuron projection0.046898583 RHOA,PAFAH1B1,PEBP1,ACTB,AK1,SOD1,DPYS L2,GLUL Table 4. Gene ontology annotation of the differentially expressed proteins of 6h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their cellular component.

9. Figure 4. Gene ontology annotation of the differentially expressed proteins of 6h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their cellular component (6H hypoxia and 6HT-NAP supplemented hypoxia).

10. 6h hypoxia Molecular Function IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO:0016491Oxidoreductase activity0.00031510728 GAPDH,SOD1,COX5A,COX6A1,PRD X5,NDUFA10,GPD1,COX5B,UQCRF S1,ALKBH3 GO:0015078 Hydrogen ion transmembrane transporter activity 0.0007035112 COX5A,COX6A1,ATP5C1,COX5B,U QCRFS1 GO:0004129 Cytochrome-c oxidase activity 0.006274329COX5A,COX6A1,COX5B GO:0016676 Oxidoreductase activity, acting on a heme group of donors, oxygen as acceptor 0.006274329COX5A,COX6A1,COX5B GO:0022890 Inorganic cation transmembrane transporter activity 0.0400635260 COX5A,COX6A1,ATP5C1,COX5B,U QCRFS1 6h hypoxia +NAP Molecular Function IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO:0016491Oxidoreductase activity0.00057112728 GAPDH,COX5A,ACADL,UQCRFS1, ALKBH3,SOD1,PRDX2,COX6A1,GS TO1,COX5B,AKR1B1,MDH1 GO:0015078 Hydrogen ion transmembrane transporter activity 0.0072715112 COX5A,UQCRFS1,COX6A1,ATP5C1, COX5B GO:0031406Carboxylic acid binding0.0079656191 ACADL,GSTP1,ALKBH3,ACOT7,DD AH1,GLUL GO:0004364 Glutathione transferase activity 0.012002322GSTP1,GSTM5,GSTO1 GO:0004129 Cytochrome-c oxidase activity 0.028039329COX5A,COX6A1,COX5B GO:0016676 Oxidoreductase activity, acting on a heme group of donors, oxygen as acceptor 0.028039329COX5A,COX6A1,COX5B Table 5. Gene ontology annotation of the differentially expressed proteins of 6h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their molecular function.

11. Figure 5. Gene ontology annotation of the differentially expressed proteins of 6h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their molecular function (6H hypoxia and 6HT-NAP supplemented hypoxia).

12. 6h hypoxia Biological Process IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO:0022904 Respiratory electron transport chain 0.0000996113 COX5A,COX6A1,ATP5C1,NDUFA10,COX5B,UQCRFS1 GO:0055114Oxidation-reduction process0.0014211934 GAPDH,SOD1,COX5A,COX6A1,ATP5C1,PR DX5,NDUFA10,GPD1,COX5B,UQCRFS1,AL KBH3 GO:0006091 Generation of precursor metabolites and energy 0.0261067443 GAPDH,COX5A,COX6A1,ATP5C1,NDUFA1 0,COX5B,UQCRFS1 GO:0019752Carboxylic acid metabolic process0.0415379862 PSMC2,GAPDH,SOD1,DDAH1,GPD1,GSTP1, PCMT1,GLUL,PSMA1 GO:0045333Cellular respiration0.0005996153 COX5A,COX6A1,ATP5C1,NDUFA10,COX5B,UQCRFS1 GO:0051592Response to calcium ion0.013939472PTK2B,GDI1,PEBP1,ACTB GO:0015980 Energy derivation by oxidation of organic compounds 0.040116318 COX5A,COX6A1,ATP5C1,NDUFA10,COX5B,UQCRFS1 GO:0017014Protein nitrosylation0.04295625GAPDH,DDAH1 6h hypoxia +NAP Biological Process IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO:0019752Carboxylic acid metabolic process0.00000117862 PSMC5,PGAM1,PSMC2,GAPDH,ENO1,ACA DL,GSTP1,CAB39,CRYM,SOD1,ACOT7,GST O1,DDAH1,PSMA6,GLUL,MDH1,PSMA1 GO:0045333Cellular respiration0.0101376153 COX5A,UQCRFS1,COX6A1,ATP5C1,COX5B,MDH1 GO:0033554Cellular response to stress0.00379513906 PSMC5,VCP,RPS6KA1,PSMC2,RHOA,ALKB H3,HBB,UBE2N,SOD1,PRDX2,AKR1B1,PSM A6,PSMA1 GO:0006091 Generation of precursor metabolites and energy 0.00119610443 PGAM1,GAPDH,ENO1,COX5A,UQCRFS1,C OX6A1,ATP5C1,GNB1,COX5B,MDH1 GO:0031400 Negative regulation of protein modification process 0.0156156165 PSMC5,PSMC2,PEBP1,PSMA6,PSMA1,IMPA CT GO:0055114Oxidation-reduction process0.00012715934 PGAM1,GAPDH,COX5A,ACADL,UQCRFS1, ALKBH3,CAB39,SOD1,PRDX2,COX6A1,AT P5C1,GNB1,COX5B,AKR1B1,MDH1 GO:0044093 Positive regulation of molecular function 0.00024114841 PSMC5,VCP,PSMC2,ENO1,RHOA,UBE2N,N ME1,SOD1,PRDX2,GNB1,RGN,GNAO1,PSM A6,PSMA1 GO:0022904 Respiratory electron transport chain 0.0345595113COX5A,UQCRFS1,COX6A1,ATP5C1,COX5B GO:0043085 Positive regulation of catalytic activity 0.00181612710 PSMC5,VCP,PSMC2,RHOA,UBE2N,SOD1,P RDX2,GNB1,RGN,GNAO1,PSMA6,PSMA1 GO:0090068 Positive regulation of cell cycle process 0.0376115115PSMC5,PSMC2,PEBP1,PSMA6,PSMA1 GO:0031398 Positive regulation of protein ubiquitination 0.0303455110PSMC5,PSMC2,UBE2N,PSMA6,PSMA1 GO:0006094Gluconeogenesis0.036151456PGAM1,GAPDH,ENO1,MDH1 GO:0055086 Nucleobase, nucleoside and nucleotide metabolic process 0.04233212968 VCP,PGAM1,PSMC2,PAFAH1B1,DNM1,AK 1,NME1,GDI1,ATP5C1,RGN,GNAO1,MDH1 Table 6. Gene ontology annotation of the differentially expressed proteins of 6h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their biological process.

13. Figure 6. Gene ontology annotation of the differentially expressed proteins of 6h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their biological process (6H hypoxia and 6HT-NAP supplemented hypoxia).

14. 12h hypoxia Cellular Component IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO:0005739Mitochondrion0221483 PSMC2,GAPDH,RHOA,COX5A,ACADL,GP D1,PARK7,PEBP1,CRYM,AK1,NME1,SOD1, PRDX2,COX6A1,ATP5C1,PRDX5,NDUFA10, DDAH1,COX5B,PPP3CA,MCL1,GLUL GO:0005625Soluble fraction013480 ENO1,RHOA,PAFAH1B1,HSPB1,GPD1,RAB 1A,PEBP1,ACTB,NME1,PPP3CA,HBA1,TPI1, GLUL GO:0043005Neuron projection0.00018411583 PTK2B,RHOA,PAFAH1B1,PARK7,PEBP1,A CTB,AK1,SYT1,CA2,SOD1,GLUL GO:0000502Proteasome complex0.000277566PSMC5,VCP,PSMC2,HSPB1,PSMA1 GO:0030424Axon0.0002958278 PTK2B,RHOA,PAFAH1B1,PARK7,PEBP1,A CTB,CA2,GLUL GO:0042995Cell projection0.002202131069 PTK2B,RHOA,PAFAH1B1,PARK7,PEBP1,A CTB,AK1,SYT1,CA2,SOD1,CDC42,GNB1,GL UL GO:0000267Cell fraction0.003168151462 VCP,ENO1,RHOA,PAFAH1B1,HSPB1,GPD1, RAB1A,PEBP1,ACTB,NME1,PPP3CA,GNAO 1,HBA1,TPI1,GLUL GO:0031966Mitochondrial membrane0.0148198478 COX5A,ACADL,PEBP1,COX6A1,ATP5C1,N DUFA10,COX5B,MCL1 12h hypoxia +NAP Cellular Component IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO:0005739Mitochondrion0.000001161483 AK1,NME1,RHOA,SOD1,PRDX2,COX6A1,C OX5A,ATP5C1,PRDX5,NDUFA10,DDAH1,G PD1,PPP3CA,PARK7,MCL1,PEBP1 GO:0005625Soluble fraction0.0000469480 ENO1,NME1,RHOA,GPD1,RAB1A,PPP3CA, PEBP1,HBA1,TPI1 GO:0043005Neuron projection0.002288583 AK1,PTK2B,SYT1,CA2,RHOA,SOD1,PARK7,PEBP1 GO:0000267Cell fraction0.012977111462 VCP,ENO1,NME1,RHOA,GPD1,RAB1A,PPP 3CA,GNAO1,PEBP1,HBA1,TPI1 GO:0030424Axon0.0309035278PTK2B,CA2,RHOA,PARK7,PEBP1 GO:0005833Hemoglobin complex0.042626213HBB,HBA1 GO:0031966Mitochondrial membrane0.0482956478 COX6A1,COX5A,ATP5C1,NDUFA10,MCL1, PEBP1 Table 7. Gene ontology annotation of the differentially expressed proteins of 12h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their cellular component.

15. Figure 7. Gene ontology annotation of the differentially expressed proteins of 12h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their cellular component (12H hypoxia and 12HT-NAP supplemented hypoxia).

16. 12h hypoxia Molecular Function IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO:0042802Identical protein binding0.00004114833 VCP,ENO1,PAFAH1B1,HSPB1,GPD1,KITLG,DNM1,CRYM,SYT1,NME1,SOD1,GSTA4,P CMT1,GLUL GO:0051920Peroxiredoxin activity0.0004138PARK7,PRDX2,PRDX5 GO:0016491Oxidoreductase activity0.00045112728 GAPDH,COX5A,ACADL,GPD1,PARK7,SOD 1,PRDX2,COX6A1,PRDX5,NDUFA10,GSTO 1,COX5B GO:0003924GTPase activity0.0015297226 RHOA,RAB1A,DNM1,CDC42,ARF1,GNB1,G NAO1 GO:0017111Nucleoside-triphosphatase activity0.00489711768 PSMC5,VCP,PSMC2,RHOA,RAB1A,DNM1,C DC42,ATP5C1,ARF1,GNB1,GNAO1 GO:0005525GTP binding0.0050938378 RHOA,RAB19,RAB1A,DNM1,NME1,CDC42, ARF1,GNAO1 GO:0019001Guanyl nucleotide binding0.006738393 RHOA,RAB19,RAB1A,DNM1,NME1,CDC42, ARF1,GNAO1 GO:0016818 Hydrolase activity, acting on acid anhydrides, in phosphorus- containing anhydrides 0.00730911802 PSMC5,VCP,PSMC2,RHOA,RAB1A,DNM1,C DC42,ATP5C1,ARF1,GNB1,GNAO1 GO:0004364Glutathione transferase activity0.010945322GSTM5,GSTA4,GSTO1 GO:0004129Cytochrome-c oxidase activity0.025578329COX5A,COX6A1,COX5B 12h hypoxia + NAP Molecular Function IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO:0051920Peroxiredoxin activity0.00009638PRDX2,PRDX5,PARK7 GO:0016776 Phosphotransferase activity, phosphate group as acceptor 0.016148340AK1,NME1,CMPK1 GO:0003924GTPase activity0.02115226RHOA,ARF1,RAB1A,GNAO1,DNM1 GO:0016491Oxidoreductase activity0.0225148728 SOD1,PRDX2,COX6A1,COX5A,PRDX5,NDU FA10,GPD1,PARK7 GO:0005525GTP binding0.0253896378NME1,RHOA,ARF1,RAB1A,GNAO1,DNM1 GO:0019001Guanyl nucleotide binding0.0313866393NME1,RHOA,ARF1,RAB1A,GNAO1,DNM1 GO:0016209Antioxidant activity0.035498352SOD1,PRDX2,PRDX5 Table 8. Gene ontology annotation of the differentially expressed proteins of 12h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their molecular function.

17. Figure 8. Gene ontology annotation of the differentially expressed proteins of 12h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their molecular function (12H hypoxia and 12HT-NAP supplemented hypoxia).

18. 12h hypoxia Biological Process IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO:0000302 Response to reactive oxygen species 0.0000587108 PTK2B,PARK7,HBB,SOD1,PRDX2,PRDX5,G NAO1 GO:0042542Response to hydrogen peroxide0.000208678PTK2B,PARK7,HBB,SOD1,PRDX2,GNAO1 GO:0006979Response to oxidative stress0.0006128225 PTK2B,PARK7,PEBP1,HBB,SOD1,PRDX2,PR DX5,GNAO1 GO:0009117Nucleotide metabolic process0.00489813921 VCP,PGAM1,PSMC2,PTK2B,PAFAH1B1,GP D1,CMPK1,DNM1,AK1,NME1,ATP5C1,GNA O1,TPI1 GO:0055114Oxidation-reduction process0.00013715934 PGAM1,GAPDH,COX5A,ACADL,GPD1,CAB 39,SOD1,PRDX2,COX6A1,ATP5C1,PRDX5,N DUFA10,GNB1,COX5B,TPI1 GO:0044093 Positive regulation of molecular function 0.00178913841 PSMC5,VCP,PSMC2,PTK2B,ENO1,RHOA,KI TLG,NME1,SOD1,PRDX2,GNB1,GNAO1,PS MA1 GO:0006800 Oxygen and reactive oxygen species metabolic process 0.018657598PTK2B,PARK7,HBB,SOD1,PRDX2 GO:0034614 Cellular response to reactive oxygen species 0.026805451HBB,SOD1,PRDX2,PRDX5 GO:0019725Cellular homeostasis0.04490610663 PTK2B,PARK7,CA2,SOD1,PRDX2,PRDX5,A RF1,GNB1,PPP3CA,MCL1 GO:0046939Nucleotide phosphorylation0.03837319CMPK1,AK1,NME1 GO:0019752Carboxylic acid metabolic process0.00000117862 PSMC5,PGAM1,PSMC2,GAPDH,ENO1,ACA DL,GPD1,CAB39,CRYM,SOD1,GSTA4,GSTO 1,DDAH1,PCMT1,TPI1,GLUL,PSMA1 GO:0006094Gluconeogenesis0.001158556PGAM1,GAPDH,ENO1,GPD1,TPI1 GO:0006091 Generation of precursor metabolites and energy 0.00128910443 PGAM1,GAPDH,ENO1,COX5A,COX6A1,AT P5C1,NDUFA10,GNB1,COX5B,TPI1 GO:0010941Regulation of cell death0.014164141179 PSMC5,VCP,PSMC2,PTK2B,RHOA,HSPB1,K ITLG,HBB,NME1,SOD1,PRDX2,PRDX5,MCL 1,PSMA1 GO:0042743 Hydrogen peroxide metabolic process 0.010052440PARK7,HBB,SOD1,PRDX2 GO:0006461Protein complex assembly0.03843110651 VCP,PTK2B,RHOA,ACADL,CAB39,HBB,SY T1,CDC42,HBA1,GLUL GO:0022904Respiratory electron transport chain0.0372345113COX5A,COX6A1,ATP5C1,NDUFA10,COX5B GO:0043085 Positive regulation of catalytic activity 0.01320811710 PSMC5,VCP,PSMC2,PTK2B,RHOA,KITLG,S OD1,PRDX2,GNB1,GNAO1,PSMA1 GO:0051259Protein oligomerization0.0229137263VCP,ACADL,CAB39,HBB,SYT1,HBA1,GLUL GO:0042592Homeostatic process0.028968131086 PTK2B,ACADL,PARK7,KITLG,CA2,SOD1,P RDX2,PRDX5,ARF1,GNB1,PPP3CA,MCL1,H BA1 Table 9a. Gene ontology annotation of the differentially expressed proteins of 12h hypoxia exposed hippocampus. The distribution of identified proteins according to their biological process.

19. 12h hypoxia +NAP Biological Process IDNameP-value Hit Count in Query List Hit Count in Genome Hit in Query List GO:0042542Response to hydrogen peroxide0.000014678HBB,PTK2B,SOD1,PRDX2,PARK7,GNAO1 GO:0006979Response to oxidative stress0.0000188225 HBB,PTK2B,SOD1,PRDX2,PRDX5,PARK7, GNAO1,PEBP1 GO:0000302 Response to reactive oxygen species 0.0000027108 HBB,PTK2B,SOD1,PRDX2,PRDX5,PARK7, GNAO1 GO:0009117Nucleotide metabolic process0.01405110921 VCP,AK1,PTK2B,NME1,ATP5C1,GPD1,GN AO1,TPI1,CMPK1,DNM1 GO:0055114Oxidation-reduction process0.0158810934 SOD1,PRDX2,COX6A1,COX5A,ATP5C1,PR DX5,NDUFA10,GPD1,TPI1,CAB39 GO:0044093 Positive regulation of molecular function 0.0471189841 VCP,PTK2B,UBE2N,ENO1,NME1,RHOA,SO D1,PRDX2,GNAO1 GO:0034614 Cellular response to reactive oxygen species 0.004258451HBB,SOD1,PRDX2,PRDX5 GO:0006800 Oxygen and reactive oxygen species metabolic process 0.001981598HBB,PTK2B,SOD1,PRDX2,PARK7 GO:0019725Cellular homeostasis0.0070849663 PTK2B,CA2,SOD1,PRDX2,PRDX5,ARF1,PP P3CA,PARK7,MCL1 GO:0046939Nucleotide phosphorylation0.009161319AK1,NME1,CMPK1 GO:0034599 Cellular response to oxidative stress 0.016999472HBB,SOD1,PRDX2,PRDX5 GO:0003013Circulatory system process0.0487336315HBB,RHOA,SOD1,GNAO1,PEBP1,HBA1 Table 9b. Gene ontology annotation of the differentially expressed proteins of 12h hypoxia with NAP supplemented hippocampus. The distribution of identified proteins according to their biological process.

20. Figure 9. Gene ontology annotation of the differentially expressed proteins of 12h hypoxia exposed and NAP supplemented hippocampus. The distribution of identified proteins according to their biological process (12H hypoxia and 12HT-NAP supplemented hypoxia).