1. Biological & Research Programs

2. Overview Status Future Direction

3. Pharmaceuticals & Personal Care Products (PPCPs) Hormones and Sterols Perfluorinated Compounds

4.  ECs identified as a top research priority ◦ Eventually, the Commission will have to make regulatory decisions regarding ECs ◦ Don’t want to be in the position of being asked what we are doing……and say “nothing”.  Be proactive

5.  Data = Informed Decisions  Conducting this research ourselves allows us to: ◦ Control the data ◦ Report the results ◦ Control what is said about the results by publishing, informing the press and interacting with the public.

6.  To date, no evidence has been found of human health effects. ◦ EPA – Global Water Research Coalition – Water Research Foundation  However, scientists are concerned over the lack of fetal exposure studies and studies involving possible effects of complex mixtures and long- term exposure.

7. 7  Effects on aquatic life are a major concern ◦ Risks for aquatic organisms much greater than humans  Continuous exposure  Exposure to higher concentration in waste streams from many different sources  Possible low dose effects  Intersex – classic example  Other reproductive effects and anomalies  Neurobehavioral  Nest guarding  Homing during migration (salmon) Fish replace the canary

8.  Do we find ECs in the Ohio River?  Are fish and other aquatic organisms showing effects possibly caused by exposure to ECs?  If YES… ◦ Develop a strategy for testing fish health indicators and develop a routine monitoring strategy.

9.  2005 ◦ Document presence of ECs ◦ Look for possible fish health effects *REVIEW*

10.  POCIS and SPMD sampling units ◦ Presence/absence only  Fish Health ◦ 200 fish sampled *REVIEW* Polar Organic Chemical Integrative Sampler - Hydrophilic Contaminants Semi-Permeable Membrane Device - Lipophilic Contaminants

11.  Hormones found ◦ In effluent mixing zones ◦ At upstream reference locations  Fish showed possible signs of health effects ◦ No Intersex found!! *REVIEW*

12.  A more comprehensive look at the occurrence of Emerging Contaminants within the mainstem Ohio River ◦ 24 Locations  Longitudinal spread  Range of potential for occurrence  POTW mixing zones  Tributaries  ‘Background’ areas ◦ 121 Pharmaceutical and Personal Care Compounds ◦ Hormones & Sterols ◦ Nonylphenol (+) ◦ Perfluorinated Compounds (PFCs) ◦ Optical Brighteners

14.  Document occurrence of ECs within the mainstem Ohio River  Identify areas of the river with a greater risk potential (to aquatic organisms) ◦ Number of contaminants present ◦ Levels observed ◦ Single chemical ‘WERF ranking’ (ongoing project)  Prioritizing Trace Organic Compounds

15.  USEPA NERL – Cincinnati ◦ APEOs – NPEOs - Optical Brighteners – PFCs ◦ Researchers are national/international experts in the EC field  AXYS Analytical ◦ Developed PPCP methods for EPA (1694; 1698)  Waters ◦ Demonstrating new analytical capabilities

16.  $84,000 Project Cost  $50,000 – River Users Program ◦ Held in ‘reserve’  $73,000 (+) available from EPA/NRSA Sampling ◦ $6K per site x 25 sites = $150,000

17.  Sampling ◦ September - October 2009  Results Available ◦ Oct. – Nov. 2009  Data assessment ◦ Dec. 2009 – Jan. 2010  Findings reported to Technical Committee ◦ Feb. 2010  Draft report/pub out for review ◦ March - April 2010

20. Phase 3

21.  Research Committee – June 2009 ◦ Priority:  The development of fish health indicators for detecting the presence of and effects from exposure to emerging contaminants.

22.  Use Phase 2 results to identify a ‘gradient of (EC) condition’ ◦ Areas with differing potential for showing biological response  Occurrence of ECs  Type  Concentration  Risk Potential – ongoing WERF Research Project  Test various candidate measures of Fish Health along the gradient identified

23.  $315,000 proposal submitted to USEPA through the Regional Methods development program. ◦ Peer review prior to submittal  Very good reviews ◦ Pulled in many different partners  USEPA  USGS  WVU  St. Cloud State  Proposal rejected on a submission technicality!  Currently searching for funding support.