2. - based on selective non-covalent interaction between an analyte and specific molecules. - is often used in biochemistry in the purification of proteins bound to tags. Affinity chromatography

3. HPLC techniques exist that do utilize affinity chromatography properties. Immobilized Metal Affinity Chromatography (IMAC) is useful to separate aforementioned molecules based on the relative affinity for the metal (I.e. Dionex IMAC). Often these columns can be loaded with different metals to create a column with a targeted affinity.

4. - a separation technique in which the mobile phase is a fluid above and relatively close to its critical temperature and pressure. Supercritical fluid chromatography

6. Ion Exchange Chromatography  commonly used in the purification of biological materials. In this type of chromatography, the use of a resin (the stationary solid phase) is used to covalently attach anions or cations onto it. Solute ions of the opposite charge in the mobile liquid phase are attracted to the resin by electrostatic forces.

7. Molecular Exclusion Chromatography - a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight.

8. Reversed-phase chromatography Reversed-phase chromatography is an elution procedure used in liquid chromatography in which the mobile phase is significantly more polar than the stationary phase.

9. Pyrolysis gas chromatography - a method of chemical analysis in which the sample is heated to decomposition to produce smaller molecules that are separated by gas chromatography and detected using mass spectrometry.

10. Chiral chromatography -- involves the separation of stereoisomers.-

11. Gravity Chromatography - Gravity chromatography is appropriate for laboratory scale and involves minimal instrumentation. It relies primarily on hand-packed columns and is particularly well suited for single-step purifications like desalting and affinity applications. Advantages of Gravity Chromatography - Simplicity — a gravity column is easy to design, set up, and operate - Minimal start up costs — costs are limited to the researchers' needs. No injectors, pumps, detectors, or fraction collectors are required - Disposable — many disposable prepack and empty polypropylene columns are available

12. Thank you! Chroma- THOR -graphy