1. What’s new in this book’s presentation of respiration? What’s confusing?

2. How many proteins are involved? What are their roles? Which metalloproteins have their own names?

3. What are the essentials of chemiosmosis? What molecule is responsible for creating the H+ gradient? What are the two requirements of chemiosmotic hypothesis?

4. How many and which metals are involved in respiration? In what types of centers are these metals in? What is the reaction that provides the energy for ATP formation?

5. matrix cytosol Cyt c c1c1 c 2 x QH 2 QH 2 2 Q Q Complex III – creates H+ gradient H+‘s are pulled in from inside for Q redn H+ ‘s are release d outside for QH2 redn

6. Fe(II)  Fe(III) Complex IV – Cytochrome C Oxidase Cu(II)  Cu(I) Cu(II)—Cu(I)  Cu(I)—Cu(I)

7. oxy peroxy Ferryl oxidized reduce d H+ limiting Complex IV – Cytochrome C Oxidase – H 2 O O-O 2- – H 2 O START Internal redox

8. Ubiquinone Stigmatellin - co-crystallized in Cyt B 1 C The magical partnership of Metal and organic redox

10. Photosystem I

11. SUN CATCHER Each lobe of clover-leaf-shaped crystal of photosystem I contains 12 different proteins, shown here in different colors with only the protein backbone of each depicted. Amid the proteins are 96 chlorophylls (pale yellow) as well as other cofactors. Photosystem I How do chl molecules behave as attennae?

12. Inside thylakoid: 4 H+ produced here Outside thylakoid: 4 H+ lost from here carboxylation regeneration reduction What are the two processes in PS and what are their names? What is the OEC?

13. Inside thylakoid: 4 H+ accumulates here Outside thylakoid: 4 H+ lost from here carboxylation regeneration reduction Net ET reaction: H2O  PSII  plastoQ  cyt b6f  plastocyanin  PSI  Fe2S2  NADP+

14. “Z-Scheme” How is the energy of light converted into chemical energy in the 1st step? How many and which metals are involved in PS?

15. Mn 4 O 4 cluster in PSII parallels Complex I in respiration Plastocyanin in PSI parallels cyt c in respiration PSI parallels Complex IV= cyt c oxidase in respiration + 4e- E ox (V) +1.2 V +0.6 V 0.0 -0.6 V -1.2 V

18. confusing?

19. What are the similarities and differences between respiration and photosynthesis?

20. 1.Cristae and thylakoid: inner membrane structures 2.Cyt bd in mitochondria ~ cyt b6f in chloroplasts 3.Small organic electron/proton carriers 4.Multi-metal site for multi electron processes 5.Reactive centers embedded in membranes 6.Creation of proton gradient 7.PSII reduces a Q to QH2; PSI is like complex IV 8.Plastocyanin functions like Cyt c 9.Use of aa residue as radical (Tyr)

21. PSII @ 3.5 Å resolution

23. Cytochorme b6f (analog of respiratory cytochrome b1c)

24. PSI Reaction Center cofactors only

25. PSI Reaction Center Chl pigments E- acceptor