1. PowerPoint ® Lecture Slides prepared by Janice Meeking, Mount Royal College C H A P T E R Copyright © 2010 Pearson Education, Inc. 7 Muscles and Muscle Tissue: Part A

2. Copyright © 2010 Pearson Education, Inc. Three Types of Muscle Tissue 1.Skeletal muscle tissue: Attached to bones and skin Striated Voluntary (i.e., conscious control) Powerful Primary topic of this chapter

3. Copyright © 2010 Pearson Education, Inc. Three Types of Muscle Tissue 2.Cardiac muscle tissue: Only in the heart Striated Involuntary More details in Chapter 18

4. Copyright © 2010 Pearson Education, Inc. Three Types of Muscle Tissue 3.Smooth muscle tissue: In the walls of hollow organs, e.g., stomach, urinary bladder, and airways Not striated Involuntary More details later in this chapter

5. Copyright © 2010 Pearson Education, Inc. Table 9.3

6. Copyright © 2010 Pearson Education, Inc. Special Characteristics of Muscle Tissue Excitability (responsiveness or irritability): ability to receive and respond to stimuli Contractility: ability to shorten when stimulated Extensibility: ability to be stretched Elasticity: ability to recoil to resting length

7. Copyright © 2010 Pearson Education, Inc. Muscle Functions 1.Movement of bones or fluids (e.g., blood) 2.Maintaining posture and body position 3.Stabilizing joints 4.Heat generation (especially skeletal muscle) 5.Protection

8. Copyright © 2010 Pearson Education, Inc. Skeletal Muscle Each muscle is served by one artery, one nerve, and one or more veins

9. Copyright © 2010 Pearson Education, Inc. Skeletal Muscle Connective tissue sheaths of skeletal muscle: Epimysium: dense regular connective tissue surrounding entire muscle Perimysium: fibrous connective tissue surrounding fascicles (groups of muscle fibers) Endomysium: fine areolar connective tissue surrounding each muscle fiber

10. Copyright © 2010 Pearson Education, Inc. Figure 9.1 Bone Perimysium Endomysium (between individual muscle fibers) Muscle fiber Fascicle (wrapped by perimysium) Epimysium Tendon Epimysium Muscle fiber in middle of a fascicle Blood vessel Perimysium Endomysium Fascicle (a) (b)

11. Copyright © 2010 Pearson Education, Inc. Microscopic Anatomy of a Skeletal Muscle Fiber Cylindrical cell 10 to 100  m in diameter, up to 30 cm long Multiple peripheral nuclei Many mitochondria Glycosomes for glycogen storage, myoglobin for O 2 storage Also contain myofibrils, sarcoplasmic reticulum, and T tubules

12. Copyright © 2010 Pearson Education, Inc. NucleusLight I bandDark A band Sarcolemma Mitochondrion (b) Diagram of part of a muscle fiber showing the myofibrils. One myofibril is extended afrom the cut end of the fiber. Myofibril

13. Copyright © 2010 Pearson Education, Inc. Ultrastructure of Thick Filament Composed of the protein myosin Myosin tails contain: 2 interwoven, heavy polypeptide chains Myosin heads contain: 2 smaller, light polypeptide chains that act as cross bridges during contraction Binding sites for actin of thin filaments Binding sites for ATP ATPase enzymes

14. Copyright © 2010 Pearson Education, Inc. Ultrastructure of Thin Filament Twisted double strand of fibrous protein actin ctin bears active sites for myosin head attachment during contraction Tropomyosin and troponin: regulatory proteins bound to actin

15. Copyright © 2010 Pearson Education, Inc. Figure 9.3 Flexible hinge region Tail Tropomyosin TroponinActin Myosin head ATP- binding site Heads Active sites for myosin attachment Actin subunits Actin-binding sites Thick filament Each thick filament consists of many myosin molecules whose heads protrude at opposite ends of the filament. Thin filament A thin filament consists of two strands of actin subunits twisted into a helix plus two types of regulatory proteins (troponin and tropomyosin). Thin filament Thick filament In the center of the sarcomere, the thick filaments lack myosin heads. Myosin heads are present only in areas of myosin-actin overlap. Longitudinal section of filaments within one sarcomere of a myofibril Portion of a thick filament Portion of a thin filament Myosin molecule Actin subunits

16. Copyright © 2010 Pearson Education, Inc. Sliding Filament Model of Contraction In the relaxed state, thin and thick filaments overlap only slightly During contraction, myosin heads bind to actin, detach, and bind again, to propel the thin filaments.

17. Copyright © 2010 Pearson Education, Inc. Requirements for Skeletal Muscle Contraction 1.Activation: neural stimulation at a neuromuscular junction 2.Excitation-contraction coupling: Generation and propagation of an action potential along the sarcolemma Final trigger: a brief rise in intracellular Ca 2+ levels

18. Copyright © 2010 Pearson Education, Inc. Events at the Neuromuscular Junction Skeletal muscles are stimulated by somatic motor neurons Axons of motor neurons travel from the central nervous system via nerves to skeletal muscles Each axon forms several branches as it enters a muscle Each axon ending forms a neuromuscular junction with a single muscle fiber

19. Copyright © 2010 Pearson Education, Inc. Figure 9.8 Nucleus Action potential (AP) Myelinated axon of motor neuron Axon terminal of neuromuscular junction Sarcolemma of the muscle fiber Ca 2+ Axon terminal of motor neuron Synaptic vesicle containing ACh Mitochondrion Synaptic cleft Fusing synaptic vesicles 1 Action potential arrives at axon terminal of motor neuron. 2 Voltage-gated Ca 2+ channels open and Ca 2+ enters the axon terminal. Figure 9.8

20. Copyright © 2010 Pearson Education, Inc. Neuromuscular Junction Axon terminal and muscle fiber are separated by a gel-filled space called the synaptic cleft Synaptic vesicles of axon terminal contain the neurotransmitter acetylcholine (ACh) Junctional folds of the sarcolemma contain ACh receptors

21. Copyright © 2010 Pearson Education, Inc. Events at the Neuromuscular Junction Nerve impulse arrives at axon terminal ACh is released and binds with receptors on the sarcolemma Electrical events lead to the generation of an action potential PLAY A&P Flix™: Events at the Neuromuscular Junction

22. Copyright © 2010 Pearson Education, Inc. Figure 9.8 Nucleus Action potential (AP) Myelinated axon of motor neuron Axon terminal of neuromuscular junction Sarcolemma of the muscle fiber Ca 2+ Axon terminal of motor neuron Synaptic vesicle containing ACh Mitochondrion Synaptic cleft Junctional folds of sarcolemma Fusing synaptic vesicles ACh Sarcoplasm of muscle fiber Postsynaptic membrane ion channel opens; ions pass. Na + K+K+ Ach – Na + K+K+ Degraded ACh Acetyl- cholinesterase Postsynaptic membrane ion channel closed; ions cannot pass. 1 Action potential arrives at axon terminal of motor neuron. 2 Voltage-gated Ca 2+ channels open and Ca 2+ enters the axon terminal. 3 Ca 2+ entry causes some synaptic vesicles to release their contents (acetylcholine) by exocytosis. 4 Acetylcholine, a neurotransmitter, diffuses across the synaptic cleft and binds to receptors in the sarcolemma. 5 ACh binding opens ion channels that allow simultaneous passage of Na + into the muscle fiber and K + out of the muscle fiber. 6 ACh effects are terminated by its enzymatic breakdown in the synaptic cleft by acetylcholinesterase.

23. Copyright © 2010 Pearson Education, Inc. Destruction of Acetylcholine ACh effects are quickly terminated by the enzyme acetylcholinesterase Prevents continued muscle fiber contraction in the absence of additional stimulation

24. Copyright © 2010 Pearson Education, Inc. Figure 9.11, step 1 Axon terminal of motor neuron Muscle fiber Triad One sarcomere Synaptic cleft Setting the stage Sarcolemma Action potential is generated Terminal cisterna of SR ACh Ca 2+

25. Copyright © 2010 Pearson Education, Inc. Figure 9.11, step 2 Action potential is propagated along the sarcolemma and down the T tubules. Steps in E-C Coupling: Troponin Tropomyosin blocking active sites Myosin Actin Active sites exposed and ready for myosin binding Ca 2+ Terminal cisterna of SR Voltage-sensitive tubule protein T tubule Ca 2+ release channel Myosin cross bridge Ca 2+ Sarcolemma Calcium ions are released. Calcium binds to troponin and removes the blocking action of tropomyosin. Contraction begins The aftermath 1 2 3 4

26. Copyright © 2010 Pearson Education, Inc. Figure 9.11, step 3 Steps in E-C Coupling: Terminal cisterna of SR Voltage-sensitive tubule protein T tubule Ca 2+ release channel Ca 2+ Sarcolemma Action potential is propagated along the sarcolemma and down the T tubules. 1

27. Copyright © 2010 Pearson Education, Inc. Figure 9.11, step 4 Steps in E-C Coupling: Terminal cisterna of SR Voltage-sensitive tubule protein T tubule Ca 2+ release channel Ca 2+ Sarcolemma Action potential is propagated along the sarcolemma and down the T tubules. Calcium ions are released. 1 2

28. Copyright © 2010 Pearson Education, Inc. Figure 9.11, step 5 TroponinTropomyosin blocking active sites Myosin Actin Ca 2+ The aftermath

29. Copyright © 2010 Pearson Education, Inc. Figure 9.11, step 6 TroponinTropomyosin blocking active sites Myosin Actin Active sites exposed and ready for myosin binding Ca 2+ Calcium binds to troponin and removes the blocking action of tropomyosin. The aftermath 3

30. Copyright © 2010 Pearson Education, Inc. Figure 9.11, step 7 TroponinTropomyosin blocking active sites Myosin Actin Active sites exposed and ready for myosin binding Ca 2+ Myosin cross bridge Calcium binds to troponin and removes the blocking action of tropomyosin. Contraction begins The aftermath 3 4

31. Copyright © 2010 Pearson Education, Inc. Figure 9.11, step 8 Action potential is propagated along the sarcolemma and down the T tubules. Steps in E-C Coupling: Troponin Tropomyosin blocking active sites Myosin Actin Active sites exposed and ready for myosin binding Ca 2+ Terminal cisterna of SR Voltage-sensitive tubule protein T tubule Ca 2+ release channel Myosin cross bridge Ca 2+ Sarcolemma Calcium ions are released. Calcium binds to troponin and removes the blocking action of tropomyosin. Contraction begins The aftermath 1 2 3 4

32. Copyright © 2010 Pearson Education, Inc. Role of Calcium (Ca 2+ ) in Contraction At low intracellular Ca 2+ concentration: Tropomyosin blocks the active sites on actin Myosin heads cannot attach to actin Muscle fiber relaxes

33. Copyright © 2010 Pearson Education, Inc. Cross Bridge Cycle Continues as long as the Ca2+ signal and adequate ATP are present Cross bridge formation—high-energy myosin head attaches to thin filament

34. Copyright © 2010 Pearson Education, Inc. Cross Bridge Cycle Cross bridge detachment—ATP attaches to myosin head and the cross bridge detaches “Cocking” of the myosin head—energy from hydrolysis of ATP cocks the myosin head into the high-energy state

35. Copyright © 2010 Pearson Education, Inc. Figure 9.12 1 Actin Cross bridge formation. Cocking of myosin head. The power (working) stroke. Cross bridge detachment. Ca 2+ Myosin cross bridge Thick filament Thin filament ADP Myosin PiPi ATP hydrolysis ATP 24 3 ADP PiPi PiPi

36. Copyright © 2010 Pearson Education, Inc. Figure 9.12, step 1 Actin Cross bridge formation. Ca 2+ Myosin cross bridge Thick filament Thin filament ADP Myosin PiPi 1

37. Copyright © 2010 Pearson Education, Inc. Figure 9.12, step 3 The power (working) stroke. ADP PiPi 2

38. Copyright © 2010 Pearson Education, Inc. Figure 9.12, step 4 Cross bridge detachment. ATP 3

39. Copyright © 2010 Pearson Education, Inc. Figure 9.12, step 5 Cocking of myosin head. ATP hydrolysis ADP PiPi 4

40. Copyright © 2010 Pearson Education, Inc. Figure 9.12 1 Actin Cross bridge formation. Cocking of myosin head. The power (working) stroke. Cross bridge detachment. Ca 2+ Myosin cross bridge Thick filament Thin filament ADP Myosin PiPi ATP hydrolysis ATP 24 3 ADP PiPi PiPi