1. A STAND-ALONE DEVICE TO SPEED ICP ANALYSES Jerry Dulude, Glass Expansion, Inc., 4 Barlows Landing Rd., Pocasset, MA 02559, jdulude@geicp.com

2. Glass Expansion www.geicp.com Sample Cycle for Unattended ICP Analysis 2 4 6 AS to sample Smpl fills line Plasma equil Integration AS to rinse Rinse 1 3 5 7 Smpl empties line

3. Glass Expansion www.geicp.com Removing Dead Volume Nebulizer – EzyFit Spray chamber – Helix Internal Standard – Low volume chamber

4. Glass Expansion www.geicp.com Software Approaches to Speeding Analysis Fast pump in rinse mode longer equilibration Predictive Rinse QC’s complicated Risk of carryover Intelligent Rinse Analyzes rinse to determine optimum time Rinse is based on sample results Modest productivity gain

5. Glass Expansion www.geicp.com Niagara™ Rapid Rinse Accessory Interfaces between ICP & Autosampler Reduces required rinse time Programmable delay time Less than 8 inches high Includes adj. height stand

6. Glass Expansion www.geicp.com How Niagara Works Analysis Rinse

7. Glass Expansion www.geicp.com Niagara Interface – no software required Started with hardware trigger on AS Recently modified to serial interface Serial cable runs from ICP computer through the AS to the Niagara. Each Niagara is preprogrammed for a specific ICP/AS combination. ICP-OES or ICP-MS Autosampler PC Niagara

8. Glass Expansion www.geicp.com Niagara Time Saving Standard SystemWith Niagara Probe to sample44 Uptake delay20 Stabilization88 Read40 Probe to rinse44 Rinse (washout)1610 Rinse (rinse)14 Total (seconds)10686 Total Rinse (seconds)38 = Rinse steps for nebulizer & spray chamber 30 second rinse10 second rinse

9. Glass Expansion www.geicp.com ICP-MS Carryover Comparison BFeZnSeMoCdSnSbTlBi 5ppm Solution5000.00 Blank112.304.291.131.002.130.893.965.321.211.27 Blank25.643.460.250.540.480.211.221.380.36 Blank33.224.540.200.340.220.100.760.790.180.20 Blank42.68-1.700.130.200.150.080.590.620.140.12 Blank51.902.160.210.000.140.070.410.440.110.13 Blank61.490.440.190.070.130.090.36 0.110.15 BFeZnSeMoCdSnSbTlBi 5ppm Solution5000.00 Blank18.971.881.060.713.130.805.149.321.071.06 Blank25.437.590.290.530.590.241.592.050.37 Blank33.166.950.270.310.350.180.971.230.250.27 Blank42.211.260.220.120.220.130.710.910.160.17 Blank52.267.330.18-0.090.130.070.490.540.110.13 Blank61.554.480.120.070.090.050.380.440.080.09 Standard method without Niagara With Niagara

10. Glass Expansion www.geicp.com ICP-MS Carryover Comparison Boron Moly Zinc Thallium

11. Glass Expansion www.geicp.com On-Line Productivity Calculator

12. Glass Expansion www.geicp.com On-Line Productivity Calculator

13. Glass Expansion www.geicp.com Payback with Niagara Without NiagaraWith Niagara Samples/hour3445 Samples/day272360 Extra samples per day088 Extra samples less QC’s079 Price per sample$50 Extra revenue per day$0$3950 Extra revenue per month$0$79,000 Extra revenue per year$0 $948,000

14. Glass Expansion www.geicp.com Summary Decrease dead-volume in sample introduction system to reduce rinse time Software approaches produce marginal productivity improvements Niagara produces clear gains in productivity leading to marked increases in instrument throughput and per instrument revenue generation.