1. Photosynthesis  Earliest life forms survived by metabolizing high-energy inorganic molecules  About 3 billion years ago, some primitive organisms evolved the ability to photosynthesize  they combined carbon dioxide and water to make glucose  further evolution resulted in more efficient photosynthesis that produced molecular oxygen as a by-product  as oxygen began to accumulate in the atmosphere, cells evolved the ability to use the oxygen for cellular respiration

2. All living things are either producers or depend on producers  most producers are photoautotrophs – absorb and convert light energy into stored chemical energy in organic molecules through the process of photosynthesis (plants, algae and cyanobacteria)  Heterotrophs – consumers and decomposers that obtain organic compounds from producers or other consumers  photosynthesis sustains almost all living things in the biosphere

3. The Visible Light Spectrum – light travels in waves  Includes all the colors of the rainbow – violet has the shortest wavelength and red has the longest

4.  Light is composed of particles or packets of energy called photons  When a pigment molecule absorbs a photon of light, one of its electrons is energized and is then accepted by an electron acceptor molecule in photosynthesis  Absorption spectrum – spectrophotometers are used to measure the relative abilities of different pigments to absorb different wavelengths of light – absorption spectrum is a plot of the absorption of light of different wavelengths

5. Action spectrum – shows how effective various wavelengths of light are in causing photosynthesis

6. Measuring the rate of photosynthesis  Production of oxygen  Uptake of carbon dioxide  Increases in biomass

7. Factors affecting the rate of photosynthesis  Light Intensity – amount of light will affect amount of products of light dependent reactions and affect overall rate of photosynthesis

8. Factors affecting the rate of photosynthesis  Concentration of CO 2 – amount of CO 2 will affect rate of photosynthesis CO 2 concentration is usually the most important limiting factor

9. Factors affecting the rate of photosynthesis  Temperature – temperature affects the rate at which the enzymes work

10. Any of these factors may be a limiting factor

12. Chloroplasts – organelles containing the green pigment, chlorophyll  Bounded by an outer and inner membrane  inner membrane encloses a fluid-filled stroma which contains the enzymes for the dark reactions  thylakoids are suspended in the stroma – fluid filled sacs (stack of thylakoids is a granum) – thylakoid membranes contain chlorophyll and the enzymes that catalyze the light reactions  Chloroplasts have their own DNA (circular chromosome) and ribosomes (70s)

13. Pigments  chlorophyll a is the main photosynthetic pigment  absorbs light mostly in the red and blue regions of the spectrum  chlorophyll a – pigment that initiates the light reactions  chlorophyll b – acts as an accessory pigment  carotenoids – other accessory pigments which are yellow and orange – absorb different wavelengths of light to broaden the spectrum of light available for photosynthesis – they pass the energy to chlorophyll a

14. Photosynthetic equation:  6CO 2 + 6H 2 O  C 6 H 12 O 6 + 6O 2  involves many steps – divided into two major sets of reactions: light-dependent reactions, and the light-independent reactions (dark reactions or carbon fixation reactions)  Light-dependent reactions take place in the thylakoid membrane  Light-independent reactions take place in the stroma

15. The Light-Dependent Reactions  Energy from the sun is temporarily stored in the molecules of ATP and NADPH – energy is then used to run the light-independent reactions

16. Chlorophyll molecules and enzymes that run the light-dependent reactions are embedded in the membranes of the thylakoids

17.  pigments are organized in the thylakoid membrane into units called antenna complexes  each antenna complex traps light and transfers the energy to a reaction center

18. Antenna complex and associated enzymes are organized into Photosystems Reaction center in Photosystem I absorbs at a peak of 700 nanometers (P700) – Photosystem II absorbs at a peak of 680 nanometers (P680)

20.  Noncyclic Photophosphorylation – both photosystems are used and electrons are ultimately passed to NADPH – electron “hole” left behind is filled by splitting water and removing electrons – O 2 gas is formed and released  continuous, one-way flow of electrons

21.  ATP is generated by chemiosmosis  called photophosphorylation because light energy is used to phosphorylate ADP called photophosphorylation because light energy is used to phosphorylate ADP called photophosphorylation because light energy is used to phosphorylate ADP

22. Light-independent reactions (Carbon Fixation Reactions or Dark reactions)  energy from ATP and NADPH is used to form glucose from CO 2  Most plants go through the Calvin (C 3 ) Cycle

24.  CO 2 enters the cycle by reacting with ribulose biphosphate (RuBP) – reaction is catalyzed by the enzyme rubisco  six carbon dioxide molecules are “fixed” to make one glucose  at the end of each cycle, RuBP is reformed