1. Author(s): Michael Hortsch, Ph.D., 2010 License: Unless otherwise noted, this material is made available under the terms of the Creative Commons Attribution – Non-Commercial 3.0 License: http://creativecommons.org/licenses/by-nc/3.0/ We have reviewed this material in accordance with U.S. Copyright Law and have tried to maximize your ability to use, share, and adapt it. The attribution key found at the end of the module provides information about how you may share and adapt this material. Copyright holders of content included in this material should contact open.michigan@umich.edu with any questions, corrections, or clarification regarding the use of content. For more information about how to cite these materials visit http://open.umich.edu/education/about/terms-of-use. Any medical information in this material is intended to inform and educate and is not a tool for self-diagnosis or a replacement for medical evaluation, advice, diagnosis or treatment by a healthcare professional. Please speak to your physician if you have questions about your medical condition. Viewer discretion is advised: Some medical content is graphic and may not be suitable for all viewers.

2. Histology of Muscle Tissue- Second Look Michael Hortsch, Ph.D. Department of Cell and Developmental Biology University of Michigan © 2010 Michael Hortsch

3. Name the structures indicated by the brackets and arrows. Indicate the borders of a full sarcomere in this EM micrograph.

4. Will the differently colored lines cut through myosin or actin or both myosin and actin polymers ? BAC

5. During a muscle contraction, which of the structures that are indicated by the white brackets will shortened ?

6. Identify this tissue.

7. Capillary

8. Identify this tissue.

10. The bracket includes how many different cells ?

11. How many muscle fibrils can you count in this muscle fiber ?

12. Name the lighter material indicated by the white arrow.

13. Identify this tissue.

17. Identify the tissue/cells shown in this EM micrograph.

18. Identify the structure in the white dashed oval.

19. In which box do you expect to find more desmosomes and adherent junctions ?

20. In which box do you expect to find more gap junctions ?

21. Identify the cell outlined by the red dashed line.

22. Identify the intracellular structures indicated by the red arrows.

23. Identify this tissue.

25. Name the two cell type, parts of which you can see in these micrographs. Name the neurotransmitter used at this type of synapse. Image courtesy of A.K. Christensen, University of Michigan

26. Identify the membranous structure indicated by the red box.

27. The membranes of the skeletal muscles cell triad belong to which two general cell organelles ?

28. Briefly describe the function of the muscle triad.

29. Can you count or estimate the number of individual muscle cells in this image ?

30. Identify the connective tissue layers that are indicated by the differently- colored arrows in each image.

31. Identify the different structures that are encircled by the dashed colored lines.

32. Myelinated axon Sensory nerve endings Identify the structure shown in this EM micrograph.

33. Name the structures that are indicated by the colored arrows. Myelinated axon Sensory nerve endings

34. Slide 3:Michigan Histology 036 - Striated muscle_001.svs Slide 4:Michigan Histology 036 - Striated muscle_001.svs Slide 5:Michigan Histology 036 - Striated muscle_001.svs Slide 6:Michigan Histology 037 - Skeletal muscle_001.svs Slide 7:Michigan Histology 034 - Skeletal muscle_001.svs Slide 8:Michigan Histology 041 - Cardiac muscle_001.svs Slide 9:Michigan Histology 207 - Small intestine_001.svs Slide 10:Michigan Histology 058thin_HISTO_83X.svs Slide 11:Michigan Histology 058thin_HISTO_83X.svs Slide 12:Michigan Histology 058thin_HISTO_83X.svs Slide 13:Michigan Histology 058L_HISTO_40X.svs Slide 14:Michigan Histology 058T_HISTO_40X.svs Slide 15:Michigan Histology 098-1_HISTO_40X.svs Slide 16:Michigan Histology 057_HISTO_40X.svs Slide 17:Michigan Histology 038 - Cardiac muscle_001.svs Slide 18:Michigan Histology 038 - Cardiac muscle_001.svs Slide 19:Michigan Histology 038 - Cardiac muscle_001.svs Slide 20:Michigan Histology 038 - Cardiac muscle_001.svs Slide 21:Michigan Histology 044 - Smooth muscle_001.svs Slide 22:Michigan Histology 044 - Smooth muscle_001.svs Slide 23:Michigan Histology 029-1_HISTO_40X.svs Slide 24:Michigan Histology 029-1_HISTO_40X.svs Slide 25:Michigan Histology 071-2A_HISTO_20X.svs neuromuscular junction 100%; Image courtesy of AK Christensen Attributions by Slide

35. Slide 26:Michigan Histology Slide 27:Michigan Histology Slide 28:Michigan Histology Slide 29:Michigan Histology 058T_HISTO_40X.svs Slide 30:Michigan Histology 059-1_HISTO_40X.svs; EM 035 - Skeletal muscle_001.svs Slide 31:Michigan Histology 071-1B_HISTO_40X.svs Slide 32:Michigan Histology 061 - Neuromuscular spindle_001.svs Slide 33:Michigan Histology 061 - Neuromuscular spindle_001.svs

36. Attribution Key for more information see: http://open.umich.edu/wiki/AttributionPolicy Use + Share + Adapt Make Your Own Assessment Creative Commons – Attribution License Creative Commons – Attribution Share Alike License Creative Commons – Attribution Noncommercial License Creative Commons – Attribution Noncommercial Share Alike License GNU – Free Documentation License Creative Commons – Zero Waiver Public Domain – Ineligible: Works that are ineligible for copyright protection in the U.S. (17 USC § 102(b)) *laws in your jurisdiction may differ Public Domain – Expired: Works that are no longer protected due to an expired copyright term. Public Domain – Government: Works that are produced by the U.S. Government. (17 USC § 105) Public Domain – Self Dedicated: Works that a copyright holder has dedicated to the public domain. Fair Use: Use of works that is determined to be Fair consistent with the U.S. Copyright Act. (17 USC § 107) *laws in your jurisdiction may differ Our determination DOES NOT mean that all uses of this 3rd-party content are Fair Uses and we DO NOT guarantee that your use of the content is Fair. To use this content you should do your own independent analysis to determine whether or not your use will be Fair. { Content the copyright holder, author, or law permits you to use, share and adapt. } { Content Open.Michigan believes can be used, shared, and adapted because it is ineligible for copyright. } { Content Open.Michigan has used under a Fair Use determination. }