1. CLONING EVALUATE THERAPEUTIC VS. REPRODUCTIVE

2. WHAT IS A CLONE? PRECISE GENETIC COPY

3. WHAT IS A CLONE?

4. http://www.sciencechannel.com/tv-shows/through-the- wormhole/videos/through-the-wormhole-human-clones.htm http://learn.genetics.utah.edu/content/cloning/whatiscloning/

5. HISTORY OF CLONING ● DOLLY o 1ST CLONED MAMMAL 1996 ● FEW SCIENTIST BELIEVE HUMAN CLONING SHOULD BE PERMITTED (REPRODUCTIVE) ● MANY SCIENTIST ARE IN FAVOR OF THERAPEUTIC CLONING o PROCEDURE USED TO PRODUCE EMBRYONIC STEM CELLS ● NO EVIDENCE OF SUCCESSFUL HUMAN CLONING

6. Dolly

7. SOMATIC CELL NUCLEAR TRANSFER (SCNT) SCNT http://www.hhmi.org/biointeractive/somatic-cell-nuclear- transfer-video

8. SCNT

9. REPRODUCTIVE VS. THERAPEUTIC IN REPRODUCTIVE CLONING THE RECONSTITUTED EMBRYO IS PLACED INTO THE UTERUS OF A SURROGATE FEMALE FOR HUMAN REPRODUCTIVE CLONING, A FEMALE WOULD DONATE HER EGGS TO BE ENUCLEATED. THE NUCLEI OF HER CELLS WOULD BE REMOVED. THE PERSON BEING CLONED WOULD DONATE SOMATIC CELLS (ANY NONREPRODUCTIVE CELL IN THE BODY) A SURROGATE MOTHER WOULD ACCEPT THE FERTILIZED OVA IN HOPES OF CARRYING ONE TO TERM

10. REPRODUCTIVE VS. THERAPEUTIC IN THERAPEUTIC CLONING, THE EMBRYO IS ALLOWED TO UNDERGO CELL DIVISION FOR SEVERAL DAYS IN THE LABORATORY UNTIL IT REACHES BLASTOCYST STAGE. A BLASTOCYST CONSISTS OF TWO LAYERS AN OUTER LAYER THAT FORMS THE PLACENTA AND OTHER TISSUES NEEDED FOR DEVELOPMENT IN THE UTERUS AND AN INNER MASS THAT FORMS ALL THE TISSUES OF THE BODY. EMBRYONIC STEM CELLS ARE OBTAINED BY OPENING THE BLASTOCYST AND PLACING THE INNER MASS IN TISSUE CULTURE. IN CULTURE THE CELLS DIVIDE AND MAKE EXACT COPIES OF THEMSELVES.

11. REPRODUCTIVE VS. THERAPEUTIC GOAL OF REPRODUCTIVE CLONING USING SCNT TO CREATE NEW ORGANISM, HUMAN OR ANIMAL. GOAL OF THERAPEUTIC CLONING USING SCNT NOT TO PRODUCE A NEW HUMAN BEING BUT TO CREATE EMBRYONIC STEM CELLS THAT ARE GENETICALLY COMPATIBLE WITH THAT OF THE RECIPIENT

12. OBSTACLES TO REPRODUCTIVE CLONING SUCCESS RATE VERY LOW FOR PRODUCING LIVE NEWBORN 3:100 CLONED ANIMALS DEVELOP PHYSICAL PROBLEMS AT EARLIER AGE THAN NORMAL “LARGE OFFSPRING SYNDROME” 20-30% LARGER NEWBORNS DIFFICULT TO DELIVER SUCH LARGE BABIES

13. Embryonic vs Adult STEM CELLS http://learn.genetics.utah.edu/content/stemcells/scintro/ http://learn.genetics.utah.edu/content/stemcells/quickref/

14. Embryonic vs Adult STEM CELLS Embryonic or pluripotent stem cells--potential to become many types of cells but not every type of cell Adult or multipotent stem cells--committed to more limited cell types with particular functions---most accessible are the blood stem cells in the bone marrow and Umbilical cord blood which may work better since they are less “mature”

15. Human STEM CELL Research Involves the use of human embryonic tissue In 2001 President George W. Bush made the following decision on federal funding for human stem cell research: 1.No funding for the derivation or use of stem cell lines taken from newly destroyed embryos 2.No funding for the creation of any human embryos for research purposes 3.No funding for the cloning of human embryos for any purposes

16. Human STEM CELL Research In 2009, President Barack Obama issued an executive order titled: Removing Barriers to Responsible Scientific Research Involving Human Stem Cells” which revoked President Bush’s order so federal government can support and conduct human stem cell research including human embryonic stem cell research as permitted by law.

17. POTENTIAL U.S. PATIENT POPULATIONS FOR STEM CELL BASED THERAPIES MEDICAL CONDITION Cardiovascular disease Autoimmune disease Diabetes Osteoporosis Cancer Alzheimer’s disease Parkinson’s disease Burns (severe) Spinal Cord Injuries Birth defects NUMBER OF PATIENTS 58 million 30 million 16 million 10 million 8.2 million 5.5 million 0.3 million 0.25 million 0.15 million/year

18. Potential Unlocking Stem Cell Potential

19. Ethical Issues Ethical issues with stem cells

20. Stem Cell Stories https://www.youtube.com/user/cellmedicine

21. Questions?