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Gene silencing in plants.
p 35S GFP LB RB PFG intron Transcription CAP Poly A DICER RISC p 35S GFP LB RB Translation CAP Poly A Transcription Fluorescence gene silencing mRNA degradation No Fluorescence RISC
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1.a. Gene silencing of transiently expressed sequences.
UV Nb p 35S GFP LB RB p 35S GFP LB RB p 35S GFP LB RB PFG intron
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1.b. Triggering gene silencing of established transgene by transient expression.
GFP UV p 35S LB RB PFG intron
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1.c. Virus-induced gene silencing (VIGS)
pTRV2 CP p 35S LB RB RZ PDS Nt Agroinfiltration PTGS PDS Sistemic PTGS pTRV1 MP 16K RpRd
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2. Systemic gene silencing.
UV GFP GFP GFP Agroinfiltration PTGS GFP Sistemic PTGS p 35S GFP LB RB PFG intron
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3.a. Suppressing gene silencing of transiently expressed sequences.
UV Nb p 35S GFP LB RB HcPro PFG intron
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3.b. Silencing of trangenes or endogenous genes cannot be established in the presence of silencing suppressor. p 35S HcPro LB RB GFP UV PFG intron
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3.c. Suppression of gene silencing mediated by virus infection.
Plant silenced for the Magnesium chelatase gene. PVY infection.
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