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Photosynthesis
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Capturing Light Energy
All energy needed by living things comes directly or indirectly from the Sun Energy moves through organisms in a living system Autotrophs vs. Heterotrophs Energy is released as bonds are broken. Some as heat the rest is stored, temporarily, as ATP. Energy is stored when bonds are made.
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ATP Energy storing compound Nucleotide structure
As phosphate is removed, energy is released from ATP. ATP--> ADP + P---> AMP + P As phosphate is added, energy is stored in ATP. AMP + P ---> ADP + P ---> ATP
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Photosynthesis Intro. Example of a biochemical pathway
6CO2 + 6H20 + Sunlight---> C6H12O6 + 6O2 + Energy Reverse reaction is Cellular Respiration (chapter 7) Use of sunlight (radiant energy) to make chemical energy (ATP) Involves Chloroplasts located in the leaves of plants 2 types of reactions take: Light and Dark reactions
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Chloroplasts Light reactions begin with the absorption of light
Contain a double membrane like mitochondria Thylakoids are individual sacs stacked to form a grana and are surrounded by the stroma Thylakoids contain pigments that absorb the different wavelengths of light Chlorophyll a and b Accessory pigments (carotenoids, xanthophylls)
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Physics of Light White light contains different wavelengths of color
Visible spectrum (light) is what is seen by the eye ROY G BIV red region has long wavelengths blue region has short wavelengths
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Light Reaction AKA: Light Dependent Reaction
Photosystems II and I are involved Light excites molecules in Photosystem II causing them to move to an electron acceptor which then passes them through an ETC to replace ‘lost’ electrons in photosystem I Light also excites molecules in Photosystem I causing them to move to an electron acceptor which then passes them through an ETC to form NADPH Hydrolysis of water allows for electrons to replace ‘lost’ electrons in photosystem II. Hydrogen atoms stay inside the thylakoid while Oxygen diffuses out of the chloroplast. 2H20---> 4H+ + 4e’ + O2
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Chemiosmosis Concentration of protons across the thylakoid causes ATP to form ATP synthase (protein located in the thylakoid membrane) harnesses energy from concentration gradient causing ADP to add another P to make ATP ATP synthase also acts like a carrier protein and an enzyme
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Recap of Light Rx Light Dependent Reactions absorb light energy in structures called thylakoids, which contain pigments. Electrons in photosystems II and I are excited and move via the ETC Water is split to replace ‘lost’ electrons for PS2 NADPH and ATP are made
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Calvin Cycle AKA: Light Independent Reaction or Dark Reaction
Products made during the Light Reaction start the Dark Reaction (biochemical pathway) Dark Reaction takes place in the stroma Carbon Dioxide is used to make organic compounds (carbon fixation) by enzyme controlled reactions
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CO2 joins a 5-carbon sugar called RUBP making a 6-carbon sugar
6-carbon sugar splits into 2 3-carbon sugars PGA ATP and NADPH from the light reaction are used to convert PGA into PGAL PGAL is converted back into RUBP using more ATP and into organic molecules
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Recap of Dark Rx ATP and NADPH from Light Reaction are used in the Dark Reaction RUBP combines with a CO2 to make 2 PGA molecules ATP then NADPH rearrange PGA into PGAL Some PGAL is used to make organic compounds; the rest to remake RUBP so cycle can continue
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Alternative Pathway C3 plants use Calvin cycle to fix Carbon Dioxide
C4 plants covert CO2 into a 4-carbon compound before going to calvin cycle (corn, sugar cane, crabgrass) CAM plants close stomata during day and open them at night fixing CO2 into organic compounds to use during the day in the calvin cycle (pineapple, cactuses)
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Rate of Photosynthesis
Rate at which a plant carries out photosynthesis is affected by the environment Light intensity Carbon Dioxide levels Temperature
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