1. Serosurveillance for monitoring immunization coverage in DHS July 24, 2015 Bernard Barrère

2.  SAMPLE and BIOMARKERS National sample of 8,400 households In all households: anthropometric measurements and hemoglobin testing among women age 15-49 and children < 5 In 1/3 households: syphilis testing among all women age 15-49 and men age 15-59 In 1/3 households: testing for immunity to tetanus among women and children <5, and immunity (or exposure) to measles among children <5 Madagascar DHS 2003-04: Overview  Measles/Tetanus testing was entirely part of the DHS Blood samples were collected by the DHS teams Testing in the labs was done according to a protocol developed with and approved by DHS Results were analyzed in collaboration with the labs and reported in the Madagascar DHS report

3. Capillary blood from finger prick was collected on filter paper (DBS) One DBS was sent to the Pasteur Institute in Antananarivo for measles testing One DBS was sent to the Baxter Health Care Lab (MD, USA) for tetanus testing Madagascar DHS 2003-04: Methodology COVERAGE FOR TETANUS/MEASLES TESTING Blood samples were collected and tested for: 91% of 2,817 eligible women 86% of 1,917 eligible children

4. Madagascar DHS 2003-04: Example of Results Distribution of children < 5 according to the Measles test result % Immunized62.8 Not immunized37.2 TOTAL100.0 Proportion immunized according to the Measles test result% Among children 12-59 months who received measles vaccination before the age of 12 months according to the vaccination card 91.2

5. SAMPLE and BIOMARKERS National sample of 18,171 households. In all households: anthropometric measurements of women age 15-49 and children < 5 In 1/2 households: Anemia testing among women age 15-49, men age 15-59, and children age 6-59 months HIV testing women age 15-49 and men age 15-59 Malaria testing (RDT and thick smear) among children age 6- 59 months DBS collected from children 6-59 months for PCR test for diagnosis of malaria and for immunity to Tetanus, Rubella, Measles, Mumps, Polio and Varicella. DR Congo DHS 2013-14: Overview (1)

6. DR Congo DHS 2013-14: Overview (2) Immunity testing was not entirely part of the DHS Blood samples were collected by the DHS teams DHS developed programs to log-in the DBS and record the test results, and merged the testing data and survey data But testing in the labs was done independently without any involvement of DHS Initially basic test results were supposed to be reported in the DRC-DHS report released in September 2014. No result was available at that time.

7. Capillary blood from finger prick was collected on filter paper (DBS) The filter papers were sent to UCLA-DRC laboratory at the National Laboratory for Biomedical Research (INRB) in Kinshasa, were they were logged in and divided in three: One DBS remained at the UCLA/INRB lab in Kinshasa for Measles, Mumps, Rubella, Varicella, and Tetanus testing One was sent to CDC Atlanta for polio testing DR Congo DHS 2013-14: Methodology COVERAGE FOR TESTING AT UCLA/INRB LAB Blood samples were collected and tested for 95.4% of 8,420 eligible children age 6-59 months

8. DR Congo DHS 2013-14: Example of Results Proportion immunized according to the Measles test result% Among children 12-23 months who received measles vaccination according to the vaccination card 69.2 Among children 12-23 months who received measles vaccination according to mother’s report 58.8 Among children 12-23 months who received measles vaccination according to the vaccination card or mother’s report 62.1 Among children 12-23 months who did NOT received measles vaccination 36.6 Among ALL tested children 12-23 months54.3

9. On-going survey As part of the on-going Mozambique HIV/Malaria survey, DBS are collected from children age 6-59 months for Tetanus and Hep B testing

11. Madagascar DHS 2003-04: Example of Results (3) Distribution of all women according to the Tetanus test result % Fully immunized (for 10 years +) (Tetanus toxoid>0.1 UI/Ml) 61.3 Partly immunized (for 2 years) (Tetanus toxoid >0.01<0.1 UI/Ml) 21.1 Not immunized17.6 TOTAL100.0 Proportion immunized according to the Tetanus test result% Among women who received at least 2 tetanus injections during last pregnancy in the past 5 years 91.6 Among women who received at least 2 tetanus injections in the past 10 years 92.7

12. Madagascar DHS 2003-04: Example of Results (2) Distribution of children < 5 according to the Tetanus test result % Fully immunized (for 10 years +) (Tetanus toxoid >0.1 UI/Ml)52.6 Party immunized (for 2 years) (Tetanus toxoid >0.01<0.1 UI/Ml)24.0 Not immunized23.4 TOTAL100.0 Proportion immunized according to the Tetanus test result% Among children 12-59 months who received three injections of DPT before the age of 12 months according to the vaccination card 91.3

13. DR Congo DHS 2013-14: Example of Results (1) Proportion of children 6-59 immunized according to the test result % Measles64.4 Tetanus36.0 Rubella34.4 Mumps22.0 Varicella9.0 Proportion immunized according to the Measles test result% Among children 12-59 months who received measles vaccination before the age of 12 months according to the vaccination card 91.2

14. DR Congo DHS 2013-14: Example of Results (3) Proportion immunized according to the Tetanus test result% Among children 12-23 months who received tetanus vaccination (3 doses) according to the vaccination card 70.7 Among children 12-23 months who received tetanus vaccination (3 doses) according to mother’s report 48.6 Among children 12-23 months who received tetanus vaccination (3 doses) according to the vaccination card or mother’s report 57.5 Among children 12-23 months who did NOT received tetanus vaccination (0 dose) 17.4 Among ALL children 12-23 months tested44.3