1. Homeostasis and Cell Transport Chapter 5 Table of Contents Section 1 Passive Transport Section 2 Active Transport

2. Section 1 Passive Transport Chapter 5 Objectives Explain how an equilibrium is established as a result of diffusion. Distinguish between diffusion and osmosis. Explain how substances cross the cell membrane through facilitated diffusion. Explain how ion channels assist the diffusion of ions across the cell membrane.

3. Section 1 Passive Transport Chapter 5 Standards SPI 3210.1.7 Predict the movement of water and other molecules across selectively permeable membranes. SPI 3210.1.8 Compare and contrast active and passive transport. 3210.1.8 Analyze experimental data to distinguish between active and passive transport. CLE 3210.1.5 Compare different models to explain the movement of materials into and out of cells.

4. Passive Transport NO ENERGY REQUIRED to move substances across membrane -- water, lipids, and other lipid soluble substances. Chapter 5 Section 1 Passive Transport Types: –Diffusion –Osmosis –Facilitated Diffusion –Filtration Involves the movement of molecules across the cell membrane without an input of energy by the cell.

5. Section 1 Passive Transport Chapter 5 Diffusion Diffusion is the movement of molecules from an area of higher concentration to an area of lower concentration, driven by the molecules’ kinetic energy until a state of dynamic equilibrium* is reached. Occurs because of Brownian Motion, i.e., the random movement of particles. *Dynamic equilibrium – continued movement of molecules with no net change in concentration –Concentration gradient, i.e., the difference in concentration across space.

6. Chapter 5 Click below to watch the Visual Concept. Concentration Gradient Section 1 Passive Transport

7. Chapter 5 Diffusion Section 1 Passive Transport

8. Chapter 5 Osmosis Osmosis is the diffusion of water across a membrane.

9. Chapter 5 Click below to watch the Visual Concept. Osmosis Section 1 Passive Transport

10. Chapter 5 Osmosis, continued Direction of Osmosis –The net direction of osmosis is determined by the relative solute concentrations on the two sides of the membrane.

11. Section 1 Passive Transport Chapter 5 Osmosis, continued Direction of Osmosis –When the solute concentration outside the cell is higher than that in the cytoplasm, the solution outside is hypertonic (more solute, less water) to the cytoplasm, and water will diffuse out of the cell. –Plasmolysis = cell’s shrinking due to water loss

12. Section 1 Passive Transport Chapter 5 Osmosis, continued Direction of Osmosis –When the solute concentration outside the cell is lower than that in the cytosol, the solution outside is hypotonic (less solute, more water) to the cytosol, and water will diffuse into the cell. –Cytolysis = cell’s bursting due to water gain

13. Section 1 Passive Transport Chapter 5 Osmosis, continued Direction of Osmosis –When the solute concentrations outside and inside the cell are equal, the solution outside is isotonic (same solute, same water), and there will be no net movement of water.

14. Chapter 5 Hypertonic, Hypotonic, Isotonic Solutions Section 1 Passive Transport

15. Chapter 5 Osmosis, continued How Cells Deal With Osmosis –To remain alive, cells must compensate for the water that enters the cell in hypotonic environments and leaves the cell in hypertonic environments. –Cells in multicellular organisms respond to hypotonic environments by pumping solutes out of the cytosol (RBCs cannot compensate for changes in solute concentration) –Contractile vacuoles are organelles that regulate water levels in paramecia.

16. Section 1 Passive Transport Chapter 5 Facilitated Diffusion Diffusion of molecules across a membrane when they are not soluble in lipids or are too large (e.g. glucose) to pass through pores in membrane In facilitated diffusion, a molecule binds to a carrier protein on one side of the cell membrane. The carrier protein (specific for one type of molecule) then changes its shape and transports the molecule down its concentration gradient to the other side of the membrane.

17. Chapter 5 Facilitated Diffusion Section 1 Passive Transport

18. Chapter 5 Diffusion Through Ion Channels Ion channels are proteins, or groups of proteins, that provide small passageways across the cell membrane through which specific ions can diffuse. Ions important in cell function include sodium, potassium, calcium, and chloride

19. Chapter 5 Ion Channels Section 1 Passive Transport

20. Section 2 Active Transport Chapter 5 Crash Course #5 Cell Membranes and Transport

21. Section 2 Active Transport Chapter 5 Objectives Distinguish between passive transport and active transport. Explain how the sodium-potassium pump operates. Compare endocytosis and exocytosis.

22. Section 1 Passive Transport Chapter 5 Standards SPI 3210.1.7 Predict the movement of water and other molecules across selectively permeable membranes. SPI 3210.1.8 Compare and contrast active and passive transport. 3210.1.8 Analyze experimental data to distinguish between active and passive transport. CLE 3210.1.5 Compare different models to explain the movement of materials into and out of cells.

23. Section 2 Active Transport Chapter 5 Active transport moves molecules across the cell membrane from an area of lower concentration to an area of higher concentration. Unlike passive transport, active transport requires cells to expend energy. Some types of active transport are performed by carrier proteins called cell membrane pumps. Cell Membrane Pumps

24. Section 2 Active Transport Chapter 5 Cell Membrane Pumps, continued Sodium-Potassium Pump –The sodium-potassium pump moves three Na + ions into the cell’s external environment for every two K + ions it moves into the cytoplasm. Animal cells must have a higher concentration of Na+ ions outside the cell and a higher concentration of K+ ions inside the cell –ATP supplies the energy that drives the pump.

25. Chapter 5 Sodium-Potassium Pump Section 2 Active Transport

26. Sodium-Potassium Pump The exchange of three Na+ ions for two K+ ions creates an electrical gradient across the cell membrane –Outside becomes positively charged relative to the inside, which becomes negative Difference in electrical charge is important for the conduction of electrical impulses along nerve cells Chapter 5 Section 2 Active Transport

27. Chapter 5 Movement in Vesicles Endocytosis –In endocytosis, cells ingest external fluid, macromolecules, and large particles, including cells by folding around them and forming a pouch. –The pouch then pinches off and becomes a membrane-bound organelle called a vesicle. *Some vesicles fuse with lysosomes, and their contents are digested by lysosomal enzymes

28. Section 2 Active Transport Chapter 5 Movement in Vesicles, continued Endocytosis –Endocytosis includes pinocytosis, in which the vesicle contains solutes or fluids, and phagocytosis, in which the vesicle contains large particles or whole cells. Bacteria and viruses are ingested in this way –Receptor-mediated endocytosis – molecules are brought into the cell via coated pits (proteins)

29. Section 2 Active Transport Chapter 5 Movement in Vesicles, continued Exocytosis –In exocytosis, vesicles made by the cell fuse with the cell membrane, releasing their contents into the external environment. Used to release large molecules, such as proteins, waste products, or toxins that would damage the cell if they were released within the cytoplasm

30. Chapter 5 Click below to watch the Visual Concept. Visual Concept Exocytosis and Endocytosis Section 2 Active Transport

31. Chapter 5 Endocytosis and Exocytosis Section 2 Active Transport